dc.contributor.author | Butt, Hussain Shakeel | |
dc.contributor.author | Ulriksen, Emilie Steinbakk | |
dc.contributor.author | Rise, Frode | |
dc.contributor.author | Wangensteen, Helle | |
dc.contributor.author | Duus, Jens Øllgaard | |
dc.contributor.author | Inngjerdingen, Marit | |
dc.contributor.author | Inngjerdingen, Kari Tvete | |
dc.date.accessioned | 2023-12-18T09:47:47Z | |
dc.date.available | 2023-12-18T09:47:47Z | |
dc.date.created | 2023-12-14T14:13:22Z | |
dc.date.issued | 2024 | |
dc.identifier.issn | 0144-8617 | |
dc.identifier.uri | https://hdl.handle.net/11250/3107979 | |
dc.description.abstract | Daphne mezereum L., an important medicinal plant in Scandinavian folk medicine, was used to treat ailments such as diarrhea, swelling and stomach pain. A range of natural compounds have been isolated, but little attention has been given to the polysaccharides in this plant. Previous work in our group have shown that a polysaccharide enriched fraction from the bark of D. mezereum exhibited pro-inflammatory effects. To pursue this further, the aim of the present work was to isolate and characterize these polysaccharides. From the ethanol-precipitate of a water extract, one neutral (DMP-NF) and one acidic (DMP-AF) fraction was isolated by anion-exchange chromatography. GC, GC–MS and 1D- and 2D-NMR were used to characterize the polysaccharide structures. DMP-NF appeared to be a mixture of arabinan, arabinogalactan and hemicelluloses such as xyloglucan, mannan and xylan. DMP-AF contained a pectic polysaccharide mainly consisting of an unusually long homogalacturonan backbone. Enzymatic treatment by pectinase of DMP-AF yielded DMP-ED, which contained a rhamnogalacturonan-I backbone with arabinan, galactan and arabinogalactan side chains. Both DMP-NF and DMP-ED induced IFN-γ and TNF-α secretion in peripheral blood mononuclear cells (PBMCs), DMP-ED being the most potent fraction. DMP-AF was less active, which might be due to a less sterically available rhamnogalacturonan-I domain. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier B. V. | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Structural elucidation of novel pro-inflammatory polysaccharides from Daphne mezereum L. | en_US |
dc.title.alternative | Structural elucidation of novel pro-inflammatory polysaccharides from Daphne mezereum L. | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.volume | 324 | en_US |
dc.source.journal | Carbohydrate Polymers | en_US |
dc.identifier.doi | 10.1016/j.carbpol.2023.121554 | |
dc.identifier.cristin | 2213697 | |
dc.source.articlenumber | 121554 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |