The plant cell wall is a structure that is essential to the growth and development of plants. It provides the plant with both structural support and a defense against the environment and herbivores. Damage to the plant cell wall can come from many sources and recently there has been an increasing amount of evidence that the plant has a sensor system that detects and initiates responses to damage done to the cell wall. ATSFH19 (AT5G47730), a gene coding for a phosphatidylinositol transfer protein in Arabidopsis thaliana, has been identified as possibly involved in the response to cell wall damage. The aim of this project was to investigate the function of ATSFH19 by phenotypical characterization of knockout plants and generated transgenic lines after cellulose biosynthesis inhibition (CBI) through staining assays and quantification of phytohormone levels as well as localizing the protein at a tissue and cell level. Here I show data that confirm the previous findings that plants with nonfunctional ATSFH19 have a distinct CBI-induced phenotype as well as showing data that suggests that the close ATSFH19 homolog ATSFH16 also has CBI-induced responses that deviate from wildtype. I also show that I was able to generate constructs ready for transformation into plants.