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dc.contributor.authorMertens, Robert
dc.contributor.authorMelchert, Sarah
dc.contributor.authorGitler, Daniel
dc.contributor.authorSchou, Morten Brix
dc.contributor.authorSæther, Sverre Georg
dc.contributor.authorVaaler, Arne
dc.contributor.authorPiepgras, Johannes
dc.contributor.authorKochova, Elena
dc.contributor.authorBenfenati, Fabio
dc.contributor.authorAhnert-Hilger, Gudrun
dc.contributor.authorRuprecht, Klemens
dc.contributor.authorHöltje, Markus
dc.identifier.citationPLOS ONE. 2018, 13:e0208636 (12), 1-14.nb_NO
dc.description.abstractObjective To identify the specific domains of the presynaptic protein synapsin targeted by recently described autoantibodies to synapsin. Methods Sera of 20 and CSF of two patients with different psychiatric and neurological disorders previously tested positive for immunoglobulin (Ig)G antibodies to full-length synapsin were screened for IgG against synapsin I domains using HEK293 cells transfected with constructs encoding different domains of rat synapsin Ia. Additionally, IgG subclasses were determined using full-length synapsin Ia. Serum and CSF from one patient were also screened for IgA autoantibodies to synapsin I domains. Sera from nine and CSF from two healthy subjects were analyzed as controls. Results IgG in serum from 12 of 20 IgG synapsin full-length positive patients, but from none of the healthy controls, bound to synapsin domains. Of these 12 sera, six bound to the A domain, five to the D domain, and one to the B- (and possibly A-), D-, and E-domains of synapsin I. IgG antibodies to the D-domain were also detected in one of the CSF samples. Determination of IgG subclasses detected IgG1 in two sera and one CSF, IgG2 in none of the samples, IgG3 in two sera, and IgG4 in eight sera. One patient known to be positive for IgA antibodies to full-length synapsin had IgA antibodies to the D-domain in serum and CSF. Conclusions Anti-synapsin autoantibodies preferentially bind to either the A- or the D-domain of synapsin I.nb_NO
dc.publisherPublic Library of Science (PLOS)nb_NO
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleEpitope specificity of anti-synapsin autoantibodies: Differential targeting of synapsin I domainsnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.source.journalPLOS ONEnb_NO
dc.description.localcodeCopyright: © 2018 Mertens et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.nb_NO
cristin.unitnamePH - Østmarka psykiatriske avdeling
cristin.unitnameInstitutt for psykisk helse

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal