Vis enkel innførsel

dc.contributor.advisorBruheim, Pernb_NO
dc.contributor.authorHaug, Anne Martenb_NO
dc.date.accessioned2014-12-19T13:14:41Z
dc.date.available2014-12-19T13:14:41Z
dc.date.created2011-10-11nb_NO
dc.date.issued2011nb_NO
dc.identifier447298nb_NO
dc.identifierntnudaim:6577nb_NO
dc.identifier.urihttp://hdl.handle.net/11250/245766
dc.description.abstractAs a part of cancer research and therapy, it is important to study metabolic responses caused by DNA damaging agents. Whereas earlier studies have focused on changes at protein levels caused by DNA damaging agents, this project focuses on establishment of sampling and cultivation protocols for metabolome analysis, using Saccharomyces cerevisiae as a model organism growing in exponential phase. Sampling and cultivations protocols were optimized before cultures were stressed by DNA damaging agent. At the time of sampling, cells and extracellular media were separated by filtration prior to quenching and extraction in 75% boiling ethanol. Solvent evaporation under reduced pressure and ambient temperature were used for metabolite concentration. Yeast cultures were exposed to osmotic stress and two concentrations of the DNA damaging agent 5-fluorouracil. Samples were taken prior to stress exposure, at the time of exposure and in intervals after stress exposure. Metabolite extracts were analyzed by GC-MS single quadrupole in respect of amino acids, citric acids cycle intermediates and fatty acids. Generally, citric acid cycle intermediates were most influenced by stress agents, followed by amino acids and fatty acids. GC-MS single quad was the only analytical method applied. For a more complete metabolic profiling, including other groups of metabolites, LC-MS/MS should be applied. This analytical method will cover groups, such glycolytic intermediates, which can not be analyzed by GC-MS, due to their volatility.nb_NO
dc.languageengnb_NO
dc.publisherInstitutt for bioteknologinb_NO
dc.subjectntnudaim:6577no_NO
dc.subjectMBIOT Bioteknologino_NO
dc.subjectno_NO
dc.titleMetabolome Studies of Stress Responses in Saccharomyces Cerevisiae : - implementation of sampling and analytical protocolsnb_NO
dc.typeMaster thesisnb_NO
dc.source.pagenumber78nb_NO
dc.contributor.departmentNorges teknisk-naturvitenskapelige universitet, Fakultet for naturvitenskap og teknologi, Institutt for bioteknologinb_NO


Tilhørende fil(er)

Thumbnail
Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel