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dc.contributor.advisorAlmaas, Eivindnb_NO
dc.contributor.authorJohansen, Sigurd Hagennb_NO
dc.date.accessioned2014-12-19T13:14:21Z
dc.date.available2014-12-19T13:14:21Z
dc.date.created2011-06-04nb_NO
dc.date.issued2011nb_NO
dc.identifier420741nb_NO
dc.identifierntnudaim:6580nb_NO
dc.identifier.urihttp://hdl.handle.net/11250/245646
dc.description.abstractIn the field of systems and synthetic biology there has been an increasing interest for the use of genetic circuits during the last decade. Several circuits have been successfully put together, many of which were based on models. During this thesis a model for a toggle switch was analysed both deterministically and stochastically.The HOM2-circuit approximation for a bistable tuneable switch from Ghim and Almaas (2009) was re-derived in order to make it asymmetrical. Deterministic analysis was conducted yielding stability diagrams, describing the phase plane showing bistability for the genetic switch. Furthermore, stochastic simulations of the approximation were conducted. This gave a somewhat narrower bistable area than the deterministic analysis, possibly due to the nature of saddle-node bifurcations. Parameter values for a switch based on experiments were estimated for the approximation, and these were used in a stochastic simulation. The result from this simulation was in correspondencewith the deterministic analysis. A stochastic simulation of the complete circuit was conducted based on parameter values found in literature. For this simulation bistability was not shown.In order to further explore the circuit, and validity of the approximation, experimental investigation is needed. This has been planned together with Rahmi Lale, PhD, and Professor Eivind Almaas at the Department of Biotechnology, NTNU.nb_NO
dc.languageengnb_NO
dc.publisherInstitutt for bioteknologinb_NO
dc.subjectntnudaim:6580no_NO
dc.subjectMBIOT Bioteknologino_NO
dc.subjectno_NO
dc.titleModelling and Analysis of a Synthetic Bistable Genetic Switchnb_NO
dc.typeMaster thesisnb_NO
dc.source.pagenumber66nb_NO
dc.contributor.departmentNorges teknisk-naturvitenskapelige universitet, Fakultet for naturvitenskap og teknologi, Institutt for bioteknologinb_NO


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