Blar i Institutt for bioteknologi og matvitenskap på forfatter "Otterlei, Marit"
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A Study of Loading of Base Excision Repair Proteins at Replication Locus
Bjørås, Karine (Master thesis, 2013)Our genome is constantly exposed to endogenous and exogenous DNA damaging factors. In order to prevent accumulation of mutations and to maintain genome integrity, cells must repair induced lesions. This occurs through a ... -
Activation of multiple stress responses in Staphylococcus aureus substantially lowers the minimal inhibitory concentration when combining two novel antibiotic drug candidates
Singleton, Amanda Holstad; Bergum, Olaug Elisabeth Torheim; Søgaard, Caroline Krogh; Røst, Lisa M.; Olsen, Cecilie Elisabeth; Blindheim, Fredrik Heen; Ræder, Synnøve Brandt; Bjørnstad, Frithjof Andreas; Sundby, Eirik; Hoff, Bård Helge; Bruheim, Per; Otterlei, Marit (Peer reviewed; Journal article, 2023)The past few decades have been plagued by an increasing number of infections caused by antibiotic resistant bacteria. To mitigate the rise in untreatable infections, we need new antibiotics with novel targets and drug ... -
Analysis of DNA Repair Proteins and Chromatin Remodeling Factors Using iPOND
Søgaard, Caroline Danielsen (Master thesis, 2012)Most chemotherapeutic treatments rely on induction of severe DNA damage to kill the cancer cells. However, DNA repair pathways can repair the induced lesions, leading to survival of the tumor cells. Thus, inhibition of DNA ... -
Anti-Cancer Potential of Homemade Fresh Garlic Extract Is Related to Increased Endoplasmic Reticulum Stress
Petrovic, Voin; Nepal, Anala; Olaisen, Camilla; Bachke, Siri; Hira, Jonathan; Søgaard, Caroline Krogh; Røst, Lisa Marie; Misund, Kristine; Andreassen, Trygve; Melø, Torun Margareta; Bartosova, Zdenka; Bruheim, Per; Otterlei, Marit (Journal article; Peer reviewed, 2018)The use of garlic and garlic-based extracts has been linked to decreased incidence of cancer in epidemiological studies. Here we examine the molecular and cellular activities of a simple homemade ethanol-based garlic extract ... -
Development and application of cultivation and analytical workflows for the study of cellular responses to antibiotics in E. coli
Thorfinnsdottir, Lilja Brekke (Doctoral theses at NTNU;2024:127, Doctoral thesis, 2024)Antibiotics revolutionized medicine during the 1900s. However, a void in new discoveries in the last decades and increased antibiotic resistance are major threats to society. New antibiotics are highly needed to cope with ... -
Effects of Hypoxia on Cell Growth and Metabolism in the Leukemia Cell Line JJN-3 and Bladder Cancer Cell Line UM-UC-3
Langørgen, Ida Eide (Master thesis, 2018)Hypoxia is a condition in which the oxygen (O2) access is extremely reduced and thus limits the O2 available for cellular respiration. The hypoxic condition induces metabolic adjustments in the cells that permit further ... -
Halogenated Pyrrolopyrimidines with Low MIC on Staphylococcus aureus and Synergistic Effects with an Antimicrobial Peptide
Olsen, Cecilie Elisabeth; Blindheim, Fredrik Heen; Søgaard, Caroline Krogh; Røst, Lise Marie; Singleton, Amanda; Bergum, Olaug Elisabeth Torheim; Bruheim, Per; Otterlei, Marit; Sundby, Eirik; Hoff, Bård Helge (Peer reviewed; Journal article, 2022)Currently, there is a world-wide rise in antibiotic resistance causing burdens to individuals and public healthcare systems. At the same time drug development is lagging behind. Therefore, finding new ways of treating ... -
Identification of Potential Cellular Pathways Involved in Mediating the Rapid Bactericidal Effects of the Novel β-clamp-targeting Antimicrobial Peptide Betatide
Wang, Emmy (Master thesis, 2023)Økende antimikrobiell resistens, samt redusert utvikling av nye antibiotika, har ført til en global utfordring som innebærer en stor økonomisk og sosial belastning. Betatide er et nytt antimikrobielt peptid som binder til ... -
Increased Anticancer Efficacy of Intravesical Mitomycin C Therapy when combined with a PCNA Targeting Peptide
Gederaas, Odrun Arna; Søgaard, Caroline Danielsen; Viset, Trond; Bachke, Siri; Bruheim, Per; Arum, Carl-Jørgen; Otterlei, Marit (Journal article; Peer reviewed, 2014)Non–muscle-invasive bladder cancers (NMIBCs) are tumors confined to the mucosa or the mucosa/submucosa. An important challenge in treatment of NMIBC is both high recurrence and high progression rates. Consequently, more ... -
Mass Spectrometric Metabolic Profiling of the Human Bladder Cancer Cell Line UM-UC-3 under Cytostatic Stress
Forsland, Yngve (Master thesis, 2016)Muscle Invasive Bladder Cancers (MIBCs) contributes to approximately 30% of bladder cancer at initial diagnosis in patients, accompanied by an only 50% five-year survival rate. The standard procedure for treating this type ... -
Nucleoside Analogues Are Potent Inducers of Pol V-mediated Mutagenesis
Sumabe, Balagra Kasim; Ræder, Synnøve Brandt; Røst, Lisa M.; Sharma, Animesh; Donkor, Eric S.; Mosi, Lydia; Duodu, Samuel; Bruheim, Per; Otterlei, Marit (Peer reviewed; Journal article, 2021)Drugs targeting DNA and RNA in mammalian cells or viruses can also affect bacteria present in the host and thereby induce the bacterial SOS system. This has the potential to increase mutagenesis and the development of ... -
Nucleoside Analogues Are Potent Inducers of Pol V-mediated Mutagenesis
Sumabe, Balagra Kasim; Ræder, Synnøve Brandt; Røst, Lisa M.; Sharma, Animesh; Donkor, Eric S.; Mosi, Lydia; Duodu, Samuel; Bruheim, Per; Otterlei, Marit (Journal article; Peer reviewed, 2021)Drugs targeting DNA and RNA in mammalian cells or viruses can also affect bacteria present in the host and thereby induce the bacterial SOS system. This has the potential to increase mutagenesis and the development of ... -
Targeted Mass Spectrometric Metabolite Profiling for Studies of Central Carbon Metabolism
Røst, Lisa M. (Doctoral theses at NTNU;2020:194, Doctoral thesis, 2020)Metabolism is the total set of enzymatic reactions occurring in a cell, tissue, organ, or organism to sustain life. It is an implementation of all upstream levels of regulation and environmental stimuli and hence reflecting ... -
The role of PCNA as a scaffold protein in cellular signaling is functionally conserved between yeast and humans
Olaisen, Camilla; Kvitvang, Hans Fredrik Nyvold; Lee, Sungmin; Almaas, Eivind; Bruheim, Per; Drabløs, Finn; Otterlei, Marit (Journal article; Peer reviewed, 2018)Proliferating cell nuclear antigen (PCNA), a member of the highly conserved DNA sliding clamp family, is an essential protein for cellular processes including DNA replication and repair. A large number of proteins from ... -
"Two hits - one stone" increased efficacy of cisplatin-based therapies by targeting PCNA's role in both DNA repair and cellular signaling
Søgaard, Caroline Krogh; Blindheim, Augun; Røst, Lisa Marie; Petrovic, Voin; Nepal, Anala; Bachke, Siri; Liabakk, Nina-Beate; Gederaas, Odrun Arna; Viset, Trond; Arum, Carl-Jørgen; Bruheim, Per; Otterlei, Marit (Journal article; Peer reviewed, 2018)Low response rate and rapid development of resistance against commonly used chemotherapeutic regimes demand new multi-targeting anti-cancer strategies. In this study, we target the stress-related roles of the scaffold ...