Browsing Institutt for bioteknologi og matvitenskap by Author "Malmo, Jostein"
Now showing items 1-7 of 7
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Advancements in Construction of XylS/Pm Expression Vectors in Gram-positive Bacteria - Evaluated in Bacillus subtilis and Lactobacillus plantarum
Istre, Mette Stausland (Master thesis, 2018)Escherichia coli (E. coli) is the most commonly used host for expression of recombinant proteins. A large fraction of recombinant proteins produced in E. coli are stored in an insoluble form, making ... -
Central carbon metabolite profiling reveals vector-associated differences in the recombinant protein production host Escherichia coli BL21
Garcia Calvo, Laura; Rane, Divyata Vilas; Everson, Nikalet; Humlebrekk, Sigurd Tømmerberg; Mathiassen, Lise Femanger; Mæhlum, Astfrid Helene Morka; Malmo, Jostein; Bruheim, Per (Peer reviewed; Journal article, 2023)The Gram-negative bacterium Escherichia coli is the most widely used host for recombinant protein production, both as an industrial expression platform and as a model system at laboratory scale. The recombinant protein ... -
Chitosan-based nanocarriers for gene- and siRNA-delivery
Malmo, Jostein (Doktoravhandlinger ved NTNU, 1503-8181; 2012:223, Doctoral thesis, 2012)Gene therapy is promising for the treatment of many currently incurable diseases by delivering or silencing specific genes with pDNA or siRNA, respectively. Clinical trials have mostly been conducted with viral or relatively ... -
Effects of Sec-Pathway Modifications on Periplasmatic Translocation of Recombinant Proteins in Escherichia coli
Elvheim, Andrea Iselin (Master thesis, 2018)Recombinant proteins are expressed for use in both research, industry and medicine. The Gram-negative bacteria Escherichia coli (E. coli) is often used as an expression host. It has several properties making it a suitable ... -
Establishing new XylS/Pm based Gram-Negative Protein Production Strains - Evaluating Pseudoalteromonas haloplanktis and Pseudomonas putida
Strypet, Goro Malene Brennmoen (Master thesis, 2018)To date, Escherichia coli is the most used bacterial host for recombinant protein production. Some proteins are, however, hard to express in this species due to a reducing intracellular environment and inclusion body ... -
Improving expression from the XylS / Pm promoter in Corynebacterium glutamicum
Krakeli, Anne Sofie (Master thesis, 2019)Ved hjelp av rekombinant DNA-teknologi kan gener overføres mellom arter og uttrykkes i andre arter enn opphavsarten. Rekombinante proteinr brukes til en rekke formål i ulike industrier, fra mat- og tekstilindustri til ... -
Translational regulation of periplasmic folding assistants and proteases as a valuable strategy to improve production of translocated recombinant proteins in Escherichia coli
Gawin, Agnieszka Maria; Ertesvåg, Helga; Hartvigsen Hansen, Sine Alise; Malmo, Jostein; Brautaset, Trygve (Peer reviewed; Journal article, 2020)Background Advantages of translocation of recombinant proteins to the periplasm in Escherichia coli include simplified downstream processing, and improved folding and in vivo activity of the target protein. There are, ...