Blar i Institutt for bioteknologi og matvitenskap på forfatter "Garcia Calvo, Laura"
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Central carbon metabolite profiling reveals vector-associated differences in the recombinant protein production host Escherichia coli BL21
Garcia Calvo, Laura; Rane, Divyata Vilas; Everson, Nikalet; Humlebrekk, Sigurd Tømmerberg; Mathiassen, Lise Femanger; Mæhlum, Astfrid Helene Morka; Malmo, Jostein; Bruheim, Per (Peer reviewed; Journal article, 2023)The Gram-negative bacterium Escherichia coli is the most widely used host for recombinant protein production, both as an industrial expression platform and as a model system at laboratory scale. The recombinant protein ... -
Experimental determination of Escherichia coli biomass composition for constraint-based metabolic modeling
Simensen, Vetle; Schulz, Christian; Karlsen, Emil; Bråtelund, Signe; Burgos, Idun Maria Tokvam; Thorfinnsdottir, Lilja Brekke; Garcia Calvo, Laura; Bruheim, Per; Almaas, Eivind (Peer reviewed; Journal article, 2022)Genome-scale metabolic models (GEMs) are mathematical representations of metabolism that allow for in silico simulation of metabolic phenotypes and capabilities. A prerequisite for these predictions is an accurate ... -
Optimized Fast Filtration-based Sampling and Extraction Enables Precise and Absolute Quantification of the Escherichia coli Central Carbon Metabolome
Thorfinnsdottir, Lilja Brekke; Garcia Calvo, Laura; Bø, Gaute Hovde; Bruheim, Per; Røst, Lisa Marie Ivarsdatter (Peer reviewed; Journal article, 2023)Precise and accurate quantification is a prerequisite for interpretation of targeted metabolomics data, but this task is challenged by the inherent instability of the analytes. The sampling, quenching, extraction, and ...