Browsing Institutt for bioteknologi og matvitenskap by Author "Aakvik, Trine"
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A new and improved host-independent plasmid system for RK2-based conjugal transfer
Aakvik, Trine; Lale, Rahmi; Degnes, Kristin Fløgstad; Lando, Malin; Valla, Svein (Journal article; Peer reviewed, 2014)Bacterial conjugation is a process that is mediated either by a direct cell-to-cell junction or by formation of a bridge between the cells. It is often used to transfer DNA constructs designed in Escherichia coli to recipient ... -
New broad-host-range Biological Tools for Metagenomics and Screening of Marine DNA Libraries
Aakvik, Trine (Doktoravhandlinger ved NTNU, 1503-8181; 2011:41, Doctoral thesis, 2011)The fact that only a minority of the microorganisms in environmental samples can be cultivated in the laboratory necessitates the use of metagenomics to better explore and exploit the huge source of genetic diversity ... -
Non-Invasive Analysis of Recombinant mRNA Stability in Escherichia coli by a Combination of Transcriptional Inducer Wash-Out and qRT-PCR
Kucharova, Veronika; Aakvik, Trine; Almaas, Eivind; Naas, Adrian Ertsås; Brautaset, Trygve; Valla, Svein (Journal article; Peer reviewed, 2013)mRNA stability is one among many parameters that can potentially affect the level of recombinant gene expression in bacteria. Blocking of the entire prokaryotic transcription machinery by addition of rifampicin is commonly ...