Browsing NTNU Open by Author "Winge, Per"
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A CRISPR/Cas9 system adapted for gene editing in marine algae
Sparstad, Torfinn; Sharma, Amit Kumar; Bones, Atle M.; Nymark, Marianne; Winge, Per (Journal article; Peer reviewed, 2016)Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens ... -
A sex-inducing pheromone triggers cell cycle arrest and mate attraction in the diatom Seminavis robusta
Moeys, Sara; Frenkel, Johannes; Lembke, Christian; Gillard, Jeroen; Devos, Valerie; Van den Berge, Koen; Bouillon, Barbara; Huysman, Marie JJ; De Decker, Sam; Scharf, Julia; Bones, Atle M.; Brembu, Tore; Winge, Per; Sabbe, Koen; Vuylsteke, Martin; Clement, Lieven; De Veylder, Lieven; Pohnert, Georg; Vyverman, Wim (Journal article; Peer reviewed, 2016)Although sexual reproduction is believed to play a major role in the high diversification rates and species richness of diatoms, a mechanistic understanding of diatom life cycle control is virtually lacking. Diatom sexual ... -
Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana
Kissen, Ralph; Øverby, Anders; Winge, Per; Bones, Atle M. (Journal article; Peer reviewed, 2016)Background Isothiocyanates (ITCs) are degradation products of the plant secondary metabolites glucosinolates (GSLs) and are known to affect human health as well as plant herbivores and pathogens. To investigate the processes ... -
An integrated analysis of molecular acclimation to high light in the marine diatom Phaeodactylum tricornutum
Nymark, Marianne; Valle, Kristin Collier; Brembu, Tore; Winge, Per; Hancke, Kasper; Andresen, Kjersti; Johnsen, Geir; Bones, Atle M. (Journal article; Peer reviewed, 2009-11-03)Photosynthetic diatoms are exposed to rapid and unpredictable changes in irradiance and spectral quality, and must be able to acclimate their light harvesting systems to varying light conditions. Molecular mechanisms behind ... -
Arabidopsis thaliana MIRO1 and MIRO2 GTPases Are Unequally Redundant in Pollen Tube Growth and Fusion of Polar Nuclei during Female Gametogenesis
Sørmo, Christopher Gravningen; Brembu, Tore; Winge, Per; Bones, Atle M. (Journal article; Peer reviewed, 2011)MIRO GTPases have evolved to regulate mitochondrial trafficking and morphology in eukaryotic organisms. A previous study showed that T-DNA insertion in the Arabidopsis MIRO1 gene is lethal during embryogenesis and affects ... -
Characterization of new putative adhesion factors in Shiga toxin-producing Escherichia coli
Adhikari, Dhurba (Master thesis, 2018)Worldwide nearly 2.8 million people are infected by STEC with acute illness, among them approximately 0.14% lead to HUS and 0.008% lead to death. Shiga toxins are essential virulence factors of STEC, but also other factors ... -
Characterization of the Cytochrome p450 Family in the unique Diatom Seminavis robusta
Strøm Midthun, Elise (Master thesis, 2012)This thesis describes the cytochrome p450 superfamily in the pennate diatom Seminavis robusta. This diatom has a benthic way of life and can adhere to surfaces, and is a new candidate model organism representing the pennate ... -
Commensal and opportunistic bacteria present in the microbiota in atlantic cod (Gadus morhua) larvae differentially alter the hosts’ innate immune responses
Vestrum, Ragnhild Inderberg; Forberg, Torunn; Luef, Birgit; Bakke, Ingrid; Winge, Per; Olsen, Yngvar; Vadstein, Olav (Journal article; Peer reviewed, 2022)The roles of host-associated bacteria have gained attention lately, and we now recognise that the microbiota is essential in processes such as digestion, development of the immune system and gut function. In this study, ... -
Creation of Phaeodactylum tricornutum mutant strains with truncated light harvesting antennas through CRISPR/Cas9 mediated knockout of members of the CpSRP pathway
Hafskjold, Marthe Caroline (Master thesis, 2017)Production of marine fatty acids by diatom cultivation has previously been proposed as a way to create a new source of omega-3 to be used in aquafeed. However, low photosynthetic productivity of diatoms in mass cultivation ... -
CRISPR/Cas9-mediated ablation of elovl2 in Atlantic salmon (Salmo salar L.) inhibits elongation of polyunsaturated fatty acids and induces Srebp-1 and target genes
Winge, Per; Datsomor, Alex; Olsen, Rolf Erik; Jin, Yang; Vik, Jon Olav; Edvardsen, Rolf; Grammes, Fabian; Wargelius, Anna; Li, Keshuai (Journal article; Peer reviewed, 2019)Atlantic salmon can synthesize polyunsaturated fatty acids (PUFAs), such as eicosapentaenoic acid (20:5n-3), arachidonic acid (20:4n-6) and docosahexaenoic acid (22:6n-3) via activities of very long chain fatty acyl elongases ... -
CRISPR/Cas9-mediated editing of Δ5 and Δ6 desaturases impairs Δ8-desaturation and docosahexaenoic acid synthesis in Atlantic salmon (Salmo salar L.)
Datsomor, Alex Kojo; Olsen, Rolf Erik; Madaro, Angelico; Bones, Atle M.; Edvardsen, Rolf Brudvik; Wargelius, Anna; Winge, Per (Journal article; Peer reviewed, 2019)The in vivo functions of Atlantic salmon fatty acyl desaturases (fads2), Δ6fads2-a, Δ6fads2-b, Δ6fads2-c and Δ5fads2 in long chain polyunsaturated fatty acid (LC-PUFA) synthesis in salmon and fish in general remains to be ... -
Dynamic responses to silicon in Thalassiosira pseudonana - Identification, characterisation and classification of signature genes and their corresponding protein motifs
Brembu, Tore; Chauton, Matilde Skogen; Winge, Per; Bones, Atle M.; Vadstein, Olav (Journal article; Peer reviewed, 2017)The diatom cell wall, or frustule, is a highly complex, three-dimensional structure consisting of nanopatterned silica as well as proteins and other organic components. While some key components have been identified, ... -
Effect of Cadmium on the marine diatoms Seminavis robusta and Phaeodactylum tricornutum: A comparative study
Madasamy Srinivasan Babu, Anitha (Master thesis, 2016)Background: Heavy metals cause a harmful effect on marine ecosystems because of their toxicity, persistence and bioaccumulation properties. Cadmium (Cd) is speculated to be a unique heavy metal due to its diverse toxic ... -
Effects of different dietary ingredients on the immune responses and antioxidant status in Atlantic salmon (Salmo salar L.): possible nutriomics approaches
Jalili, Mahsa (Doctoral theses at NTNU;2020:123, Doctoral thesis, 2020)The importance of salmon feeds Farming of Atlantic salmon (Salmo salar L.) is a first-rank aquaculture industry in Norway, accounting for a production of over 1.2 million tons per year. Dependency on fish meal has been ... -
epegRNA Screening Platform for Primary Immunodeficiency Mutations in HEK293 cells and Hematopoietic Stem and Progenitory Cells (HSPCs)
Ganeshan, Janaarthan (Master thesis, 2024)Mangel på adenosindeaminase (DADA2) er en primær immunsvikt forårsaket av autosomale recessive mutasjoner i adenosindeaminasegen (CECR1, ADA2). Korrekte behandlingsmetoder er først og fremst avhengig av sykdomsfenotypen, ... -
Gene regulation of carbon fixation, storage, and utilization in the diatom phaeodactylum tricornutum acclimated to light/dark cycles
Chauton, Matilde Skogen; Winge, Per; Brembu, Tore; Vadstein, Olav; Bones, Atle M. (Journal article; Peer reviewed, 2013)The regulation of carbon metabolism in the diatom Phaeodactylum tricornutum at the cell, metabolite, and gene expression levels in exponential fed-batch cultures is reported. Transcriptional profiles and cell chemistry ... -
Genome editing in diatoms: achievements and goals
Kroth, Peter G.; Bones, Atle M.; Daboussi, Fayza; Ferrante, Maria I.; Jaubert, Marianne; Kolot, Misha; Nymark, Marianne; Rio Bártulos, Carolina; Ritter, Andrés; Russo, Monia T.; Serif, Manuel; Winge, Per; Falciatore, Angela (Journal article; Peer reviewed, 2018)Diatoms are major components of phytoplankton and play a key role in the ecology of aquatic ecosystems. These algae are of great scientific importance for a wide variety of research areas, ranging from marine ecology and ... -
Genome editing of marine algae: Technology development and use of the CRISPR/Cas9 system for studies of light harvesting complexes and regulation of phosphate homeostasis
Sharma, Amit Kumar (Doctoral theses at NTNU;2020:160, Doctoral thesis, 2020) -
Genome-Wide Transcript Profiling of Endosperm without Paternal Contribution Identifies Parent-of-Origin-Dependent Regulation of AGAMOUS-LIKE36
Shirzadi, Reza; Andersen, Ellen Dehnes; Bjerkan, Katrine; Gloeckle, Barbara M; Heese, Maren; Ungru, Alexander; Winge, Per; Koncz, C; Aalen, Reidunn B.; Schnittger, Arp; Grini, Paul Eivind (Journal article; Peer reviewed, 2011)Seed development in angiosperms is dependent on the interplay among different transcriptional programs operating in the embryo, the endosperm, and the maternally-derived seed coat. In angiosperms, the embryo and the endosperm ... -
IIb-RAD-sequencing coupled with random forest classification indicates regional population structuring and sex-specific differentiation in salmon lice (Lepeophtheirus salmonis)
Guragain, Prashanna; Båtnes, Anna Solvang; Zobolas, John; Olsen, Yngvar; Bones, Atle M.; Winge, Per (Peer reviewed; Journal article, 2022)The aquaculture industry has been dealing with salmon lice problems forming serious threats to salmonid farming. Several treatment approaches have been used to control the parasite. Treatment effectiveness must be optimized, ...