• 5-hydroxymethylcytosine marks mammalian origins acting as a barrier to replication 

      Prikrylova, Terezia; Robertson, Julia; Ferrucci, Francesca; Konorska, Dorota Joanna; Aanes, Håvard; Manaf, Adeel; Zhang, Beibei; Vågbø, Cathrine Broberg; Kusnierczyk, Anna; Gilljam, Karin Margaretha; Løvkvam-Køster, Caroline; Otterlei, Marit; Dahl, John Arne; Enserink, Jorrit; Klungland, Arne; Robertson, Adam Brian (Journal article; Peer reviewed, 2019)
      In most mammalian cells, DNA replication occurs once, and only once between cell divisions. Replication initiation is a highly regulated process with redundant mechanisms that prevent errant initiation events. In lower ...
    • A novel method for the efficient and selective identification of 5-hydroxymethylcytosine in genomic DNA 

      Robertson, Adam Brian; Dahl, John Arne; Vågbø, Cathrine Broberg; Tripathi, P; Krokan, Hans Einar; Klungland, Arne (Journal article; Peer reviewed, 2011)
      Recently, 5-hydroxymethylcytosine (5hmC) was identified in mammalian genomic DNA. The biological role of this modification remains unclear; however, identifying the genomic location of this modified base will assist in ...
    • A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair 

      Brickner, Joshua R; Soll, Jennifer M; Lombardi, Patrick M; Vågbø, Cathrine Broberg; Mudge, Miranda C; Oyeniran, Clement; Rabe, Renana; Jackson, Jessica; Sullender, Meagan E; Blazosky, Elyse; Byrum, Andrea K; Zhao, Yu; Corbett, Mark A.; Gècz, Jozef; Field, Michael; Vindigni, Alessandro; Slupphaug, Geir; Wolberger, Cynthia; Mosammaparast, Nima (Journal article; Peer reviewed, 2017)
      DNA repair is essential to prevent the cytotoxic or mutagenic effects of various types of DNA lesions, which are sensed by distinct pathways to recruit repair factors specific to the damage type. Although biochemical ...
    • ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA 

      Van den Born, Erwin; Vågbø, Cathrine Broberg; Songe-Møller, Lene; Leihne, VIbeke; Lien, Guro Flor; Leszczynska, G; Malkiewicz, A; Krokan, Hans Einar; Kirpekar, F; Klungland, Arne; Falnes, Pål (Journal article; Peer reviewed, 2011)
      Mammals have nine different homologues (ALKBH1–9) of the Escherichia coli DNA repair demethylase AlkB. ALKBH2 is a genuine DNA repair enzyme, but the in vivo function of the other ALKBH proteins has remained elusive. It ...
    • Biochemical reconstitution of TET1-TDG-BER-dependent active DNA demethylation reveals a highly coordinated mechanism 

      Weber, Alain R.; Krawczyk, Claudia; Robertson, Adam Brian; Kusnierczyk, Anna; Vågbø, Cathrine Broberg; Schürmann, David; Klungland, Arne; Schär, Primo (Peer reviewed; Journal article, 2016)
      Cytosine methylation in CpG dinucleotides is an epigenetic DNA modification dynamically established and maintained by DNA methyltransferases and demethylases. Molecular mechanisms of active DNA demethylation began to surface ...
    • Changes of 5-hydroxymethylcytosine distribution during myeloid and lymphoid differentiation of CD34+ cells 

      Tekpli, Xavier; Urbanucci, Alfonso; Hashim, Adnan; Vågbø, Cathrine Broberg; Lyle, Robert; Kringen, Marianne K.; Staff, Anne Cathrine; Dybedal, Ingunn; Mills, Ian Geoffrey; Klungland, Arne; Staerk, Judith (Journal article; Peer reviewed, 2016)
      Background Hematopoietic stem cell renewal and differentiation are regulated through epigenetic processes. The conversion of 5-methylcytosine into 5-hydroxymethylcytosine (5hmC) by ten-eleven-translocation enzymes provides ...
    • Direct repair of alkylation damage in DNA and RNA by  2-oxoglutarate- and iron-dependent dioxygenases 

      Vågbø, Cathrine Broberg (Doktoravhandlinger ved NTNU, 1503-8181; 2008:232, Doctoral thesis, 2008)
      Direkte reparasjon av alkyleringsskader i DNA og RNA av 2-oksoglutarat- og jernavhengige dioksygenaser All informasjon nødvendig for at en levende organisme skal utvikle seg og fungere er lagret i organismens arvestoff, ...
    • DNA base modifications in honey bee and fruit fly genomes suggest an active demethylation machinery with species- and tissue-specific turnover rates 

      Rasmussen, Erik; Vågbø, Cathrine Broberg; Münch, Daniel; Krokan, Hans Einar; Klungland, Arne; Amdam, Gro Vang; Dahl, John Arne (Journal article; Peer reviewed, 2016)
      Well-known epigenetic DNA modifications in mammals include the addition of a methyl group and a hydroxyl group to cytosine, resulting in 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) respectively. In contrast, ...
    • Genome-wide profiling of DNA 5-hydroxymethylcytosine during rat Sertoli cell maturation 

      Landfors, Miriam; Johansen, Jostein; Aronsen, Jan Magnus; Vågbø, Cathrine Broberg; Doré, Louis C.; He, Chuan; Sjaastad, Ivar; Sætrom, Pål; Fedorcsák, Péter; Dahl, John Arne; Aanes, Håvard; Fusser, Markus; Klungland, Arne (Journal article; Peer reviewed, 2017)
      Sertoli cells have dual roles during the cells’ lifetime. In the juvenile mammal, Sertoli cells proliferate and create the structure of the testis, and during puberty they cease to proliferate and take on the adult role ...
    • Protozoan ALKBH8 Oxygenases Display both DNA Repair and tRNA Modification Activities 

      Zdzalik, Daria; Vågbø, Cathrine Broberg; Kirpekar, F; Davydova, Erna; Puścian, Alicja; Maciejewska, Agnieszka M.; Krokan, Hans Einar; Klungland, Arne; Tudek, Barbara; Van den Born, Erwin; Falnes, Pål (Journal article; Peer reviewed, 2014)
      The ALKBH family of Fe(II) and 2-oxoglutarate dependent oxygenases comprises enzymes that display sequence homology to AlkB from E. coli, a DNA repair enzyme that uses an oxidative mechanism to dealkylate methyl and etheno ...
    • Recessive Truncating Mutations in ALKBH8 Cause Intellectual Disability and Severe Impairment of Wobble Uridine Modification 

      Vågbø, Cathrine Broberg; Monies, Dorota; Al-Owain, Mohammad; Alhomaidi, Suzan; Alkuraya, Fowzan S. (Journal article; Peer reviewed, 2019)
      The wobble hypothesis was proposed to explain the presence of fewer tRNAs than possible codons. The wobble nucleoside position in the anticodon stem-loop undergoes a number of modifications that help maintain the efficiency ...
    • Roles of Trm9-and ALKBH8-like proteins in the formation of modified wobble uridines in Arabidopsis tRNA 

      Leihne, VIbeke; Kirpekar, F; Vågbø, Cathrine Broberg; Van den Born, Erwin; Krokan, Hans Einar; Grini, Paul Eivind; Meza, Trine Johansen; Falnes, Pål (Journal article; Peer reviewed, 2011)
      Uridine at the wobble position of tRNA is usually modified, and modification is required for accurate and efficient protein translation. In eukaryotes, wobble uridines are modified into 5-methoxycarbonylmethyluridine (mcm ...
    • Synergistic Actions of Ogg1 and Mutyh DNA Glycosylases Modulate Anxiety-like Behavior in Mice 

      Bjørge, Monica Dahl; Hildrestrand, Gunn Annette; Scheffler, Katja; Suganthan, Rajikala; Rolseth, Veslemøy; Kusnierczyk, Anna; Rowe, Alexander D.; Vågbø, Cathrine Broberg; Vetlesen, Susanne; Eide, Lars; Slupphaug, Geir; Yusaku, Nakabeppu; Timothy, Bredy; Klungland, Arne; Bjørås, Magnar (Journal article; Peer reviewed, 2015)
      Ogg1 and Mutyh DNA glycosylases cooperate to prevent mutations caused by 8-oxoG, a major premutagenic DNA lesion associated with cognitive decline. We have examined behavior and cognitive function in mice deficient of these ...
    • The DNA dioxygenase ALKBH2 protects Arabidopsis thaliana against methylation damage 

      Meza, Trine Johansen; Moen, Marivi; Vågbø, Cathrine Broberg; Krokan, Hans Einar; Klungland, Arne; Grini, Paul Eivind; Falnes, Pål (Journal article; Peer reviewed, 2012)
      The Escherichia coli AlkB protein (EcAlkB) is a DNA repair enzyme which reverses methylation damage such as 1-methyladenine (1-meA) and 3-methylcytosine (3-meC). The mammalian AlkB homologues ALKBH2 and ALKBH3 display ...
    • The human methyltransferase ZCCHC4 catalyses N6-methyladenosine modification of 28S ribosomal RNA 

      Pinto, Rita; Vågbø, Cathrine Broberg; Jakobsson, Magnus; Yeji, Kim; Baltissen, Marijke P; O'Donohue, Marie-Francoise; Guzman, Ulises H; Malecki, Jedrzej Mieczyslaw; Wu, Jie; Kirpekar, Finn; Olsen, Jesper V; Gleizes, Pierre-Emmanuel; Vermeulen, Michiel; Leidel, Sebastian A.; Slupphaug, Geir; Falnes, Pål Øystein (Journal article; Peer reviewed, 2019)
      RNA methylations are essential both for RNA structure and function, and are introduced by a number of distinct methyltransferases (MTases). In recent years, N6-methyladenosine (m6A) modification of eukaryotic mRNA has been ...
    • UNG-initiated base excision repair is the major repair route for 5-fluorouracil in DNA, but 5-fluorouracil cytotoxicity depends mainly on RNA incorporation 

      Pettersen, Henrik Sahlin; Visnes, Torkild; Vågbø, Cathrine Broberg; Svaasand, Eva K; Doseth, Berit; Slupphaug, Geir; Kavli, Bodil Merete; Krokan, Hans Einar (Journal article; Peer reviewed, 2011)
      Cytotoxicity of 5-fluorouracil (FU) and 5-fluoro-2′-deoxyuridine (FdUrd) due to DNA fragmentation during DNA repair has been proposed as an alternative to effects from thymidylate synthase (TS) inhibition or RNA incorporation. ...