Browsing NTNU Open by Author "Vågbø, Cathrine Broberg"

Now showing items 1-20 of 24

    • 5-hydroxymethylcytosine marks mammalian origins acting as a barrier to replication 

      Prikrylova, Terezia; Robertson, Julia; Ferrucci, Francesca; Konorska, Dorota Joanna; Aanes, Håvard; Manaf, Adeel; Zhang, Beibei; Vågbø, Cathrine Broberg; Kusnierczyk, Anna; Gilljam, Karin Margaretha; Løvkvam-Køster, Caroline; Otterlei, Marit; Dahl, John Arne; Enserink, Jorrit; Klungland, Arne; Robertson, Adam Brian (Journal article; Peer reviewed, 2019)
      In most mammalian cells, DNA replication occurs once, and only once between cell divisions. Replication initiation is a highly regulated process with redundant mechanisms that prevent errant initiation events. In lower ...

    • A novel method for the efficient and selective identification of 5-hydroxymethylcytosine in genomic DNA 

      Robertson, Adam Brian; Dahl, John Arne; Vågbø, Cathrine Broberg; Tripathi, P; Krokan, Hans Einar; Klungland, Arne (Journal article; Peer reviewed, 2011)
      Recently, 5-hydroxymethylcytosine (5hmC) was identified in mammalian genomic DNA. The biological role of this modification remains unclear; however, identifying the genomic location of this modified base will assist in ...

    • A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair 

      Brickner, Joshua R; Soll, Jennifer M; Lombardi, Patrick M; Vågbø, Cathrine Broberg; Mudge, Miranda C; Oyeniran, Clement; Rabe, Renana; Jackson, Jessica; Sullender, Meagan E; Blazosky, Elyse; Byrum, Andrea K; Zhao, Yu; Corbett, Mark A.; Gècz, Jozef; Field, Michael; Vindigni, Alessandro; Slupphaug, Geir; Wolberger, Cynthia; Mosammaparast, Nima (Journal article; Peer reviewed, 2017)
      DNA repair is essential to prevent the cytotoxic or mutagenic effects of various types of DNA lesions, which are sensed by distinct pathways to recruit repair factors specific to the damage type. Although biochemical ...

    • ALKBH3 partner ASCC3 mediates P-body formation and selective clearance of MMS-induced 1-methyladenosine and 3-methylcytosine from mRNA 

      Wollen, Kristian Lied; Hagen, Lars; Vågbø, Cathrine Broberg; Rabe, Renana; Iveland, Tobias Solli; Aas, Per Arne; Sharma, Animesh; Sporsheim, Bjørnar; Erlandsen, Hilde O.; Palibrk, Vuk; Bjørås, Magnar; Fonseca, Davi de Miranda; Mosammaparast, Nima; Slupphaug, Geir (Peer reviewed; Journal article, 2021)
      Background: Reversible enzymatic methylation of mammalian mRNA is widespread and serves crucial regulatory functions, but little is known to what degree chemical alkylators mediate overlapping modifcations and whether cells ...

    • ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA 

      Van den Born, Erwin; Vågbø, Cathrine Broberg; Songe-Møller, Lene; Leihne, VIbeke; Lien, Guro Flor; Leszczynska, G; Malkiewicz, A; Krokan, Hans Einar; Kirpekar, F; Klungland, Arne; Falnes, Pål (Journal article; Peer reviewed, 2011)
      Mammals have nine different homologues (ALKBH1–9) of the Escherichia coli DNA repair demethylase AlkB. ALKBH2 is a genuine DNA repair enzyme, but the in vivo function of the other ALKBH proteins has remained elusive. It ...

    • Biochemical reconstitution of TET1-TDG-BER-dependent active DNA demethylation reveals a highly coordinated mechanism 

      Weber, Alain R.; Krawczyk, Claudia; Robertson, Adam Brian; Kusnierczyk, Anna; Vågbø, Cathrine Broberg; Schürmann, David; Klungland, Arne; Schär, Primo (Peer reviewed; Journal article, 2016)
      Cytosine methylation in CpG dinucleotides is an epigenetic DNA modification dynamically established and maintained by DNA methyltransferases and demethylases. Molecular mechanisms of active DNA demethylation began to surface ...

    • Changes of 5-hydroxymethylcytosine distribution during myeloid and lymphoid differentiation of CD34+ cells 

      Tekpli, Xavier; Urbanucci, Alfonso; Hashim, Adnan; Vågbø, Cathrine Broberg; Lyle, Robert; Kringen, Marianne K.; Staff, Anne Cathrine; Dybedal, Ingunn; Mills, Ian Geoffrey; Klungland, Arne; Staerk, Judith (Journal article; Peer reviewed, 2016)
      Background Hematopoietic stem cell renewal and differentiation are regulated through epigenetic processes. The conversion of 5-methylcytosine into 5-hydroxymethylcytosine (5hmC) by ten-eleven-translocation enzymes provides ...

    • Direct repair of alkylation damage in DNA and RNA by  2-oxoglutarate- and iron-dependent dioxygenases 

      Vågbø, Cathrine Broberg (Doktoravhandlinger ved NTNU, 1503-8181; 2008:232, Doctoral thesis, 2008)
      Direkte reparasjon av alkyleringsskader i DNA og RNA av 2-oksoglutarat- og jernavhengige dioksygenaser All informasjon nødvendig for at en levende organisme skal utvikle seg og fungere er lagret i organismens arvestoff, ...

    • DNA base modifications in honey bee and fruit fly genomes suggest an active demethylation machinery with species- and tissue-specific turnover rates 

      Rasmussen, Erik; Vågbø, Cathrine Broberg; Münch, Daniel; Krokan, Hans Einar; Klungland, Arne; Amdam, Gro Vang; Dahl, John Arne (Journal article; Peer reviewed, 2016)
      Well-known epigenetic DNA modifications in mammals include the addition of a methyl group and a hydroxyl group to cytosine, resulting in 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) respectively. In contrast, ...

    • Essential roles of RNA cap-proximal ribose methylation in mammalian embryonic development and fertility 

      Dohnalkova, Michaela; Krasnykov, Kyrylo; Mendel, Mateusz; Li, Lingyun; Panasenko, Olesya; Fleury-Olela, Fabienne; Vågbø, Cathrine Broberg; Homolka, David; Pillai, Ramesh S. (Peer reviewed; Journal article, 2023)
      Eukaryotic RNA pol II transcripts are capped at the 5′ end by the methylated guanosine (m7G) moiety. In higher eukaryotes, CMTR1 and CMTR2 catalyze cap-proximal ribose methylations on the first (cap1) and second (cap2) ...

    • Genome-wide profiling of DNA 5-hydroxymethylcytosine during rat Sertoli cell maturation 

      Landfors, Miriam; Johansen, Jostein; Aronsen, Jan Magnus; Vågbø, Cathrine Broberg; Doré, Louis C.; He, Chuan; Sjaastad, Ivar; Sætrom, Pål; Fedorcsák, Péter; Dahl, John Arne; Aanes, Håvard; Fusser, Markus; Klungland, Arne (Journal article; Peer reviewed, 2017)
      Sertoli cells have dual roles during the cells’ lifetime. In the juvenile mammal, Sertoli cells proliferate and create the structure of the testis, and during puberty they cease to proliferate and take on the adult role ...

    • The Mammalian Cap-Specific m<sup>6</sup>Am RNA Methyltransferase PCIF1 Regulates Transcript Levels in Mouse Tissues 

      Pandey, Radha Raman; Delfino, Elena; Homolka, David; Roithova, Adriana; Chen, Kuan-Ming; Li, Lingyun; Franco, Giulia; Vågbø, Cathrine Broberg; Taillebourg, Emmanuel; Fauvarque, Marie-Odile; Pillai, Ramesh S. (Peer reviewed; Journal article, 2020)
      The 5′ end of eukaryotic mRNAs is protected by the m7G-cap structure. The transcription start site nucleotide is ribose methylated (Nm) in many eukaryotes, whereas an adenosine at this position is further methylated at the ...

    • Mre11 exonuclease activity removes the chain-terminating nucleoside analog gemcitabine from the nascent strand during DNA replication 

      Boeckemeier, L; Kraehenbuehl, R; Keszthelyi, A; Gasasira, MU; Vernon, EG; Beardmore, R; Vågbø, Cathrine Broberg; Chaplin, D; Gollins, S; Krokan, Hans Einar; Lambert, SAE; Paizs, B; Hartsuiker, Edgar (Peer reviewed; Journal article, 2020)
      The Mre11 nuclease is involved in early responses to DNA damage, often mediated by its role in DNA end processing. MRE11 mutations and aberrant expression are associated with carcinogenesis and cancer treatment outcomes. ...

    • Proteome alterations associated with transformation of multiple myeloma to secondary plasma cell leukemia 

      Zatula, Alexey; Dikic, Aida; Mulder, Teska Celine; Sharma, Animesh; Vågbø, Cathrine Broberg; Sousa, Mirta; Waage, Anders; Slupphaug, Geir (Peer reviewed; Journal article, 2017)
      Plasma cell leukemia is a rare and aggressive plasma cell neoplasm that may either originate de novo (primary PCL) or by leukemic transformation of multiple myeloma (MM) to secondary PCL (sPCL). The prognosis of sPCL is ...

    • Protozoan ALKBH8 Oxygenases Display both DNA Repair and tRNA Modification Activities 

      Zdzalik, Daria; Vågbø, Cathrine Broberg; Kirpekar, F; Davydova, Erna; Puścian, Alicja; Maciejewska, Agnieszka M.; Krokan, Hans Einar; Klungland, Arne; Tudek, Barbara; Van den Born, Erwin; Falnes, Pål (Journal article; Peer reviewed, 2014)
      The ALKBH family of Fe(II) and 2-oxoglutarate dependent oxygenases comprises enzymes that display sequence homology to AlkB from E. coli, a DNA repair enzyme that uses an oxidative mechanism to dealkylate methyl and etheno ...

    • Quantification of guanine-derived urinary biomarkers of oxidative stress in saturation divers by LC-MS/MS 

      Lund, Hedda Juni (Master thesis, 2022)
      Metningsdykkere jobber under konstant trykksatte omgivelser med forhøyet deltrykk av oksygen for opp til flere uker. Dette kan føre til oksidativt stress, hvilket er assosiert med en rekke sykdommer, både akutte og kroniske. ...

    • Recessive Truncating Mutations in ALKBH8 Cause Intellectual Disability and Severe Impairment of Wobble Uridine Modification 

      Vågbø, Cathrine Broberg; Monies, Dorota; Al-Owain, Mohammad; Alhomaidi, Suzan; Alkuraya, Fowzan S. (Journal article; Peer reviewed, 2019)
      The wobble hypothesis was proposed to explain the presence of fewer tRNAs than possible codons. The wobble nucleoside position in the anticodon stem-loop undergoes a number of modifications that help maintain the efficiency ...

    • RNA in DNA repair 

      Vågbø, Cathrine Broberg; Slupphaug, Geir (Peer reviewed; Journal article, 2020)
      Our genome is constantly subject to damage from exogenous and endogenous sources, and cells respond to such damage by initiating a DNA damage response (DDR). Failure to induce an adequate DDR can result in increased mutation ...

    • Roles of Trm9-and ALKBH8-like proteins in the formation of modified wobble uridines in Arabidopsis tRNA 

      Leihne, VIbeke; Kirpekar, F; Vågbø, Cathrine Broberg; Van den Born, Erwin; Krokan, Hans Einar; Grini, Paul Eivind; Meza, Trine Johansen; Falnes, Pål (Journal article; Peer reviewed, 2011)
      Uridine at the wobble position of tRNA is usually modified, and modification is required for accurate and efficient protein translation. In eukaryotes, wobble uridines are modified into 5-methoxycarbonylmethyluridine (mcm ...

    • Splice site m<sup>6</sup>A methylation prevents binding of U2AF35 to inhibit RNA splicing 

      Mendel, Mateusz; Delaney, Kamila; Pandey, Radha Raman; Chen, Kuan-Ming; Wenda, Joanna M.; Vågbø, Cathrine Broberg; Steiner, Florian A.; Homolka, David; Pillai, Ramesh S. (Journal article; Peer reviewed, 2021)
      The N6-methyladenosine (m6A) RNA modification is used widely to alter the fate of mRNAs. Here we demonstrate that the C. elegans writer METT-10 (the ortholog of mouse METTL16) deposits an m6A mark on the 3′ splice site ...