Blar i NTNU Open på forfatter "Nilsson, Per"
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Acute heart failure following myocardial infarction: complement activation correlates with the severity of heart failure in patients developing cardiogenic shock
Orrem, Hilde Lang; Nilsson, Per; Pischke, Søren Erik; Grindheim, Guro; Garred, Peter; Seljeflot, Ingebjørg; Husebye, Trygve Guttorm; Aukrust, Pål; Yndestad, Arne; Andersen, Geir Øystein; Barratt-Due, Andreas; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2018)Aims Heart failure (HF) is an impending complication to myocardial infarction. We hypothesized that the degree of complement activation reflects severity of HF following acute myocardial infarction. Methods and ... -
Bypassing adverse injection reactions to nanoparticles through shape modification and attachment to erythrocytes
Wibroe, Peter Popp; Anselmo, Aaron C; Nilsson, Per; Sarode, Apoorva; Gupta, Vivek; Urbanics, Rudolf; Szebeni, Janos; Hunter, Alan Christy; Mitragotri, Samir; Mollnes, Tom Eirik; Moghimi, Seyed Moein (Journal article, 2017)Intravenously injected nanopharmaceuticals, including PEGylated nanoparticles, induce adverse cardiopulmonary reactions in sensitive human subjects, and these reactions are highly reproducible in pigs. Although the underlying ... -
Combined Inhibition of C5 and CD14 Attenuates Systemic Inflammation in a Piglet Model of Meconium Aspiration Syndrome
Thomas, Anub Mathew; Schjalm, Camilla; Nilsson, Per; Lindenskov, Paal Helge H.; Rørtveit, Runa; Solberg, Rønnaug; Saugstad, Ola Didrik; Berglund, Magnus M.; Strömberg, Patrik; Lau, Corinna; Espevik, Terje; Jansen, Johan Høgset; Castellheim, Albert; Mollnes, Tom Eirik; Barratt-Due, Andreas (Journal article; Peer reviewed, 2018)Background: Meconium aspiration syndrome (MAS) is a severe lung condition affecting newborns and it can lead to a systemic inflammatory response. We previously documented complement activation and cytokine release in a ... -
Complement component C3 and the TLR co-receptor CD14 are not involved in angiotensin II induced cardiac remodelling
Shahini, Negar; Schjalm, Camilla; Nilsson, Per; Holt, Margrethe Flesvig; Øgaard, Jonas; Lien, Egil; Ahmed, Muhammad Shakil; Attramadal, Håvard; Aukrust, Pål; Yndestad, Arne; Mollnes, Tom Eirik; Louwe, Mieke C. (Peer reviewed; Journal article, 2020)Inflammation is centrally involved in the development of cardiac hypertrophy and the processes of remodelling. The complement system and Toll-like receptor (TLR) family, two upstream arms of the innate immune system, have ... -
Complement component C5 and TLR molecule CD14 mediate heme-induced thromboinflammation in human blood
Thomas, Anub Mathew; Gerogianni, Alexandra; McAdam, Martin Berner; Fløisand, Yngvar; Lau, Corinna; Espevik, Terje; Nilsson, Per; Mollnes, Tom Eirik; Barratt-Due, Andreas (Journal article; Peer reviewed, 2019)Heme is a critical danger molecule liberated from hemeproteins in various conditions, including from hemoglobin in hemolytic diseases. Heme may cause thromboinflammatory damage by activating inflammatory and hemostatic ... -
A conformational change of complement C5 is required for thrombin-mediated cleavage, revealed by a novel ex vivo human whole blood model preserving full thrombin activity
Nilsson, Per; Johnson, Christina; Quach, Huy Quang; Macpherson, Alex; Durrant, Oliver; Pischke, Soeren; Fure, Hilde; Landsem, Anne; Bergseth, Grete; Schjalm, Camilla; Haugaard-Kedström, Linda M.; Huber-Lang, Markus; van den Elsen, Jean; Brekke, Ole-Lars; Mollnes, Tom Eirik (Peer reviewed; Journal article, 2021)Thrombin activation of C5 connects thrombosis to inflammation. Complement research in whole blood ex vivo necessitates anticoagulation, which potentially interferes with the inflammatory modulation by thrombin. We challenged ... -
Dual inhibition of complement and Toll-like receptors as a novel approach to treat inflammatory diseases-C3 or C5 emerge together with CD14 as promising targets
Barratt-Due, Andreas; Pischke, Søren Erik; Nilsson, Per; Espevik, Terje; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2016)The host is protected by pattern recognition systems, including complement and TLRs, which are closely cross-talking. If improperly activated, these systems might induce tissue damage and disease. Inhibition of single ... -
Eculizumab-C5 complexes express a C5a neoepitope in vivo: Consequences for interpretation of patient complement analyses
Nilsson, Per; Thomas, Anub Mathew; Bergseth, Grete; Gustavsen, Alice; Volokhina, Elena B.; van den Heuvel, Lambertus P.; Barratt-Due, Andreas; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2017)The complement system has obtained renewed clinical focus due to increasing number of patients treated with eculizumab, a monoclonal antibody inhibiting cleavage of C5 into C5a and C5b. The FDA approved indications are ... -
Effects of gamma radiation sterilization on the structural and biological properties of decellularized corneal xenografts
Islam, Mohammad Mirazul; Sharifi, Roholah; Mamodaly, Shamina; Islam, Rakibul; Nahra, Daniel; Abusamra, Dina B.; Hui, Pui Chuen; Adibnia, Yashar; Goulamaly, Mehdi; Paschalis, Eleftherios I.; Cruzat, Andrea; Kong, Jing; Nilsson, Per; Argüeso, Pablo; Mollnes, Tom Eirik; Chodosh, James; Dohlman, Claes H.; Gonzalez-Andrades, Miguel (Journal article; Peer reviewed, 2019)To address the shortcomings associated with corneal transplants, substantial efforts have been focused on developing new modalities such as xenotransplantion. Xenogeneic corneas are anatomically and biomechanically similar ... -
Human endothelial cell activation by Escherichia coli and staphylococcus aureus is mediated by TNF and IL-1β secondarily to activation of C5 and CD14 in whole blood
Nymo, Stig Haugset; Gustavsen, Alice; Nilsson, Per; Lau, Corinna; Espevik, Terje; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2016)Endothelial cells (EC) play a central role in inflammation. E-selectin and ICAM-1 expression are essential for leukocyte recruitmentand are good markers of EC activation. Most studies of EC activation are done in vitro ... -
IL-6 receptor inhibition by tocilizumab attenuated expression of C5a receptor 1 and 2 in non-ST-elevation myocardial infarction
Orrem, Hilde Lang; Nilsson, Per; Pischke, Søren Erik; Kleveland, Ola; Yndestad, Arne; Ekholt, Karin; Damås, Jan Kristian; Espevik, Terje; Bendz, Bjørn; Halvorsen, Bente; Gregersen, Ida; Wiseth, Rune; Andersen, Geir Øystein; Ueland, Thor; Gullestad, Lars; Aukrust, Pål; Barratt-Due, Andreas; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2018)Background: Elevated interleukin-6 (IL-6) and complement activation are associated with detrimental effects of inflammation in coronary artery disease (CAD). The complement anaphylatoxins C5a and C3a interact with their ... -
Increased complement factor B and Bb levels are associated with mortality in patients with severe aortic stenosis
Shahini, Negar; Ueland, Thor; Auensen, Andreas; Michelsen, Annika; Ludviksen, Judith K; Hussain, Amjad Iqbal; Pettersen, Kjell; Aakhus, Svend; Espeland, Torvald; Lunde, Ida Gjervold; Kirschfink, Michael; Nilsson, Per; Mollnes, Tom Eirik; Gullestad, Lars; Aukrust, Pål; Yndestad, Arne; Louwe, Maria Cornelia (Journal article; Peer reviewed, 2019)Inflammation is involved in initiation and progression of aortic stenosis (AS). However, the role of the complement system, a crucial component of innate immunity in AS, is unclear. We hypothesized that circulating levels ... -
Inflammatory response after laparoscopic versus open resection of colorectal liver metastases: Data from the oslo-comet trial
Fretland, Åsmund Avdem; Sokolov, Andrey; Postriganova, Nadezda; Kazaryan, Airazat M.; Pischke, Søren Erik; Nilsson, Per; Rognes, Ingrid Nygren; Bjørnbeth, Bjørn Atle; Fagerland, Morten; Mollnes, Tom Eirik; Edwin, Bjørn (Journal article; Peer reviewed, 2015)Laparoscopic and open liver resection have not been compared in randomized trials. The aim of the current study was to compare the inflammatory response after laparoscopic and open resection of colorectal liver metastases ... -
Properdin binding to complement activating surfaces depends on initial C3b deposition
Harboe, Morten; Johnson, Christina; Nymo, Stig Haugset; Ekholt, Karin; Schjalm, Camilla; Lindstad, Julie Katrine; Pharo, Anne Margrethe; Hellerud, Bernt C; Ekdahl, Kristina Nilsson; Mollnes, Tom Eirik; Nilsson, Per (Journal article; Peer reviewed, 2017)Two functions have been assigned to properdin; stabilization of the alternative convertase, C3bBb, is well accepted, whereas the role of properdin as pattern recognition molecule is controversial. The presence of ... -
Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay
Nilsson, Per; Pettersen, Kristin; Oppermann, Martin; Skjeflo, Espen Waage; Fure, Hilde; Christiansen, Dorte; Mollnes, Tom Eirik (Peer reviewed; Journal article, 2021)Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for ... -
Rickettsia conorii is a potent complement activator in vivo and combined inhibition of complement and CD14 is required for attenuation of the cytokine response ex vivo
Otterdal, Kari; Portillo, Aránzazu; Astrup, Elisabeth; Ludviksen, Judith K; Schjalm, Camilla; Raoult, Didier; Olano, Juan Pablo; Halvorsen, Bente; Oteo Revuelta, José Antonio; Aukrust, Pål; Mollnes, Tom Eirik; Nilsson, Per (Journal article, 2016)Mediterranean spotted fever caused by Rickettsia conorii is a potentially lethal disease characterized by vascular inflammation affecting multiple organs. Studies of R. conorii so far have focused on activation of inflammatory ... -
Soluble IL-1 receptor 2 is associated with left ventricular remodelling in patients with ST-elevation myocardial infarction
Orrem, Hilde Lang; Shetelig, Christian; Ueland, Thor; Limalanathan, Shanmuganathan; Nilsson, Per; Husebye, Trygve Guttorm; Aukrust, Pål; Seljeflot, Ingebjørg; HOFFMANN, PAVEL; Eritsland, Jan; Mollnes, Tom Eirik; Andersen, Geir Øystein; Yndestad, Arne (Journal article; Peer reviewed, 2018)Background The inflammatory response following myocardial infarction (MI) is prerequisite for proper healing of infarcted tissue, but can also have detrimental effects on cardiac function. Interleukin (IL)-1α and IL-1β ... -
Sputter deposition of titanium on poly(Methyl methacrylate) enhances corneal biocompatibility
Sharifi, Sina; Islam, Mohammad Mirazul; Sharifi, Hannah; Islam, Rakibul; Nilsson, Per; Dohlman, Claes H.; Mollnes, Tom Eirik; Paschalis, Eleftherios I.; Chodosh, James (Peer reviewed; Journal article, 2020)Purpose: To evaluate titanium (Ti) sputtering of the poly(methyl methacrylate) (PMMA) stem of the Boston Keratoprosthesis (BK) as a method to enhance interfacial adhesion between the PMMA and the recipient corneal tissue. ... -
The alternative complement pathway is dysregulated in patients with chronic heart failure
Shahini, Negar; Michelsen, Annika; Nilsson, Per; Ekholt, Karin; Gullestad, Lars; Broch, Kaspar; Dahl, Christen Peder; Aukrust, Pål; Ueland, Thor; Mollnes, Tom Eirik; Yndestad, Arne; Louwe, Maria Cornelia (Journal article; Peer reviewed, 2017)The complement system, an important arm of the innate immune system, is activated in heart failure (HF). We hypothesized that HF patients are characterized by an imbalance of alternative amplification loop components; ... -
Tuning gelatin-based hydrogel towards bioadhesive ocular tissue engineering applications
Sharifi, Sina; Islam, Mohammad Mirazul; Sharifi, Hannah; Islam, Rakibul; Koza, Darrell; Reyes-Ortega, Felisa; Alba-Molina, David; Nilsson, Per; Dohlman, Claes H.; Mollnes, Tom Eirik; Chodosh, James; Gonzalez-Andrades, Miguel (Peer reviewed; Journal article, 2021)Gelatin based adhesives have been used in the last decades in different biomedical applications due to the excellent biocompatibility, easy processability, transparency, non-toxicity, and reasonable mechanical properties ...