Browsing NTNU Open by Author "Mollnes, Tom Eirik"
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Acute heart failure following myocardial infarction: complement activation correlates with the severity of heart failure in patients developing cardiogenic shock
Orrem, Hilde Lang; Nilsson, Per; Pischke, Søren Erik; Grindheim, Guro; Garred, Peter; Seljeflot, Ingebjørg; Husebye, Trygve Guttorm; Aukrust, Pål; Yndestad, Arne; Andersen, Geir Øystein; Barratt-Due, Andreas; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2018)Aims Heart failure (HF) is an impending complication to myocardial infarction. We hypothesized that the degree of complement activation reflects severity of HF following acute myocardial infarction. Methods and ... -
Age and Sex-Associated Changes of Complement Activity and Complement Levels in a Healthy Caucasian Population
Gaya de Costa, Mariana; Poppelaars, Felix; van Kooten, Cees; Mollnes, Tom Eirik; Tedesco, Francesco; Würzner, Reinhard; Trouw, Leendert A.; Truedsson, Lennart; Daha, Mohamed R.; Roos, Anja; Seelen, Marc A. (Journal article; Peer reviewed, 2018)Introduction: The complement system is essential for an adequate immune response. Much attention has been given to the role of complement in disease. However, to better understand complement in pathology, it is crucial to ... -
Air Bubbles Activate Complement and Trigger Hemostasis and C3-Dependent Cytokine Release Ex Vivo in Human Whole Blood
Storm, Benjamin; Christiansen, Dorte; Fure, Hilde; Ludviksen, Judith K; Lau, Corinna; Lambris, John D.; Woodruff, Trent M.; Brekke, Ole-Lars; Braaten, Tonje Bjørndal; Nielsen, Erik Waage; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2021)Venous air embolism, which may complicate medical and surgical procedures, activates complement and triggers thromboinflammation. In lepirudin-anticoagulated human whole blood, we examined the effect of air bubbles on ... -
Alginate microbeads are coagulation compatible, while alginate microcapsules activate coagulation secondary to complement or directly through FXII
Gravastrand, Caroline; Hamad, Shamal; Fure, Hilde; Steinkjer, Bjørg; Ryan, Liv; Oberholzer, José; Lambris, John D.; Lacík, Igor; Mollnes, Tom Eirik; Brekke, Ole-Lars; Rokstad, Anne Mari (Journal article; Peer reviewed, 2017)Alginate microspheres are presently under evaluation for future cell-based therapy. Their ability to induce harmful host reactions needs to be identified for developing the most suitable devices and efficient prevention ... -
Alginate microbeads are complement compatible, in contrast to polycation containing microcapsules, as revealed in a human whole blood model
Rokstad, Anne Mari; Brekke, Ole Lars; Steinkjer, Bjørg; Ryan, Liv; Kolláriková, Gabriela; Strand, Berit Løkensgard; Skjåk-Bræk, Gudmund; Lacík, Igor; Espevik, Terje; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2011)Alginate microbeads and microcapsules are presently under evaluation for future cell-based therapy. Defining their inflammatory properties with regard to humans is therefore essential. A lepirudine-based human whole blood ... -
Alginate microsphere compositions dictate different mechanisms of complement activation with consequences for cytokine release and leukocyte activation
Ørning, Mathias Pontus; Hoem, Kine Samseth; Coron, Abba Elizabeth; Skjåk-Bræk, Gudmund; Mollnes, Tom Eirik; Brekke, Ole Lars; Espevik, Terje; Rokstad, Anne Mari (Journal article; Peer reviewed, 2016)The inflammatory potential of 12 types of alginate-based microspheres was assessed in a human whole blood model. The inflammatory potential could be categorized from low to high based on the four main alginate microsphere ... -
The allosteric modulation of complement c5 by knob domain peptides
Macpherson, Alex; Laabei, Maisem; Ahdash, Zainab; Graewert, Melissa Ann; Birtley, James R.; Schulze, Monika-Sarah E.D.; Crennell, Susan; Robinson, Sarah A.; Holmes, Ben; Oleinikovas, Vladas; Nilsson, Per H.; Snowden, James; Ellis, Victoria; Mollnes, Tom Eirik; Deane, Charlotte M.; Svergun, Dmitri I.; Lawson, Alastair D.G.; van den Elsen, Jean (Peer reviewed; Journal article, 2021)Bovines have evolved a subset of antibodies with ultra-long heavy chain complementarity determining regions that harbour cysteine-rich knob domains. To produce high-affinity peptides, we previously isolated autonomous 3–6 ... -
The Alternative Complement Pathway Is Activated Without a Corresponding Terminal Pathway Activation in Patients With Heart Failure
Shahini, Negar; Bjørkelund, Elisabeth; Bendz, Christina; Massey, Richard J; Schjalm, Camilla; Halvorsen, Bente; Broch, Kaspar; Ueland, Thor; Gullestad, Lars; Nilsson, Per H.; Aukrust, Pål; Mollnes, Tom Eirik; Louwe, Maria Cornelia (Journal article; Peer reviewed, 2021)Objective Dysregulation of the complement system has been described in patients with heart failure (HF). However, data on the alternative pathway are scarce and it is unknown if levels of factor B (FB) and the C3 convertase ... -
Anti-inflammatory effects of non-statin low-density lipoprotein cholesterol-lowering drugs: an unused potential?
Hovland, Anders; Retterstøl, Kjetil; Mollnes, Tom Eirik; Halvorsen, Bente Evy; Aukrust, Pål; Lappegård, Knut Tore (Peer reviewed; Journal article, 2020)Objectives. Inflammatory responses are closely knit with low-density lipoprotein (LDL)-cholesterol in driving atherosclerosis. Even if LDL-cholesterol is causative to atherosclerotic diseases and LDL-cholesterol lowering ... -
Avoiding ambient air in test tubes during incubations of human whole-blood minimizes complement background activation
Storm, Benjamin; Christiansen, Dorte; Mollnes, Tom Eirik; Nielsen, Erik Waage (Peer reviewed; Journal article, 2020)Background In vitro, the complement system can be studied in test tubes incubated with anticoagulated human whole-blood. Background activation of complement may mask inflammatory signals. Air bubbles are known to activate ... -
Biocompatibility and Biotolerability Assessment of Microspheres Using a Whole Blood Model
Rokstad, Anne Mari; Strand, Berit Løkensgard; Espevik, Terje; Mollnes, Tom Eirik (Journal article, 2013)The whole blood model is a powerful method to determine the immediate inflammatory reactions towards foreign objects in general. This review focuses on the use of a lepirudin based whole blood model for evaluating microspheres ... -
Biphasic release of the alarmin high mobility group box 1 protein early after trauma predicts poor clinical outcome
Ottestad, William Arne; Rognes, Ingrid Nygren; Pischke, Søren; Mollnes, Tom Eirik; Andersson, Ulf; Eken, Torsten (Journal article; Peer reviewed, 2019)Objectives: The causal role of the prototype alarmin high mobility group box 1 protein in systemic inflammation and remote organ injury after trauma and shock is established in animal models but not in humans. Our aim ... -
Bypassing adverse injection reactions to nanoparticles through shape modification and attachment to erythrocytes
Wibroe, Peter Popp; Anselmo, Aaron C; Nilsson, Per; Sarode, Apoorva; Gupta, Vivek; Urbanics, Rudolf; Szebeni, Janos; Hunter, Alan Christy; Mitragotri, Samir; Mollnes, Tom Eirik; Moghimi, Seyed Moein (Journal article, 2017)Intravenously injected nanopharmaceuticals, including PEGylated nanoparticles, induce adverse cardiopulmonary reactions in sensitive human subjects, and these reactions are highly reproducible in pigs. Although the underlying ... -
C-C Motif Ligand 20 (CCL20) and C-C Motif Chemokine Receptor 6 (CCR6) in Human Peripheral Blood Mononuclear Cells: Dysregulated in Ulcerative Colitis and a Potential Role for CCL20 in IL-1β Release
Skovdahl, Helene Kolstad; Damås, Jan Kristian; Granlund, Atle van Beelen; Østvik, Ann Elisabet; Doseth, Berit; Bruland, Torunn; Mollnes, Tom Eirik; Sandvik, Arne Kristian (Journal article; Peer reviewed, 2018)The chemokine C-C motif ligand 20 (CCL20) is increased in the colonic mucosa during active inflammatory bowel disease (IBD) and can be found both in the epithelium and immune cells in the lamina propria. The present study ... -
CD14 and complement crosstalk and largely mediate the transcriptional response to Escherichia coli in human whole blood as revealed by DNA microarray
Lau, Corinna; Nygård, Ståle; Fure, Hilde; Olstad, Ole Kristoffer; Holden, Marit; Lappegård, Knut Tore; Brekke, Ole Lars; Espevik, Terje; Hovig, Johannes Eivind; Mollnes, Tom Eirik (Journal article, 2015)Systemic inflammation like in sepsis is still lacking specific diagnostic markers and effective therapeutics. The first line of defense against intruding pathogens and endogenous damage signals is pattern recognition by ... -
CD14 inhibition improves survival and attenuates thrombo-inflammation and cardiopulmonary dysfunction in a baboon model of Escherichia coli sepsis
Keshari, Ravi Shankar; Silasi, Robert; Popescu, Narcis Ioan; Regmi, Girija; Chaaban, Hala; Lambris, John D.; Lupu, Cristina; Mollnes, Tom Eirik; Lupu, Florea (Peer reviewed; Journal article, 2020)Background During sepsis, gram‐negative bacteria induce robust inflammation primarily via lipopolysacharride (LPS) signaling through TLR4, a process that involves the glycosylphosphatidylinositol (GPI)‐anchored receptor ... -
Changes in cytokines during treatment of elderly, hospitalized psychiatric patients - a naturalistic study
Bugge, Erlend; Wynn, Rolf; Mollnes, Tom Eirik; Reitan, Solveig Merete Klæbo; Lapid, Maria; Grønli, Ole Kristian (Journal article; Peer reviewed, 2019)Immunological abnormalities have been demonstrated in several psychiatric disorders. Predominantly, studies have focused on younger adults, and research on elderly psychiatric in-patients is scant. In this naturalistic ... -
Chimeric Anti-CD14 IGG2/4 Hybrid Antibodies for Therapeutic Intervention in Pig and Human Models of Inflammation
Lau, Corinna; Gunnarsen, Kristin Støen; Høydahl, Lene Støkken; Andersen, Jan Terje; Berntzen, Gøril; Pharo, Anne Margrethe; Lindstad, Julie Katrine; Ludviksen, Judith K; Brekke, Ole Lars; Barratt-Due, Andreas; Nielsen, Erik Waage; Stokes, Christopher R.; Espevik, Terje; Sandlie, Inger; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2013)CD14 is a key recognition molecule of innate immune responses, interacting with several TLRs. TLR signaling cross-talks extensively with the complement system, and combined CD14 and complement inhibition has been proved ... -
Choice of immunoassay to evaluate porcine cytokine levels
Ueland, Nora Lamark; Ludvigsen, Judith K.; Hellerud, Bernt C; Mollnes, Tom Eirik; Skjeflo, Espen Waage (Peer reviewed; Journal article, 2020)Background In order to adequately monitor cytokines in experimental models, currently available methods and commercially available kits should be compared. Aim To compare the plasma and tissue concentrations of IL-1β, ... -
Cholesterol crystal-induced endothelial cell activation is complement-dependent and mediated by TNF
Nymo, Stig Haugset; Niyonzima, Nathalie; Espevik, Terje; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2014)Cholesterol crystals are known to be a hallmark of atherosclerosis with recent studies demonstrating deposition of these crystals in early fatty streak formation as well as penetrating the intima following plaque rupture. ...