Browsing NTNU Open by Author "Bruheim, Per"
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A Study of Loading of Base Excision Repair Proteins at Replication Locus
Bjørås, Karine (Master thesis, 2013)Our genome is constantly exposed to endogenous and exogenous DNA damaging factors. In order to prevent accumulation of mutations and to maintain genome integrity, cells must repair induced lesions. This occurs through a ... -
A Study of Metabolites in Breast Cancer Xenografts: Optimisation of Extraction Method for MS-based Analysis and Investigation of Subtypes using Metabolite Profiling and Isotope Labelling
Pedersen, Ine (Master thesis, 2012)The most common cancer among women in Europe and the United States is breast cancer. However, treatment remains a major challenge. If prediction of prognosis and treatment response become more reliable, treatment can be ... -
A translational medicine approach to hypertensive nephropathy: Prevalence, diagnosis, and pathophysiology
Øvrehus, Marius Altern (Doctoral theses at NTNU;2018:400, Doctoral thesis, 2018)NORSK SAMMENDRAG Translasjonsmedisinske studier av hypertensiv nefropati: Prevalens, diagnose og patofysiologi Kronisk nyresjukdom defineres som nedsatt nyrefunksjon (estimert glomerulær filtrasjonsrate (eGFR) <60 ... -
Absolute Quantification of the Central Carbon Metabolome in Eight Commonly Applied Prokaryotic and Eukaryotic Model Systems
Røst, Lisa Marie; Thorfinnsdottir, Lilja Brekke; Kumar, Kanhaiya; Fuchino, Katsuya; Langørgen, Ida Eide; Bartosova, Zdenka; Kristiansen, Kåre Andre; Bruheim, Per (Peer reviewed; Journal article, 2020)Absolute quantification of intracellular metabolite pools is a prerequisite for modeling and in-depth biological interpretation of metabolomics data. It is the final step of an elaborate metabolomics workflow, with challenges ... -
Activation of multiple stress responses in Staphylococcus aureus substantially lowers the minimal inhibitory concentration when combining two novel antibiotic drug candidates
Singleton, Amanda Holstad; Bergum, Olaug Elisabeth Torheim; Søgaard, Caroline Krogh; Røst, Lisa M.; Olsen, Cecilie Elisabeth; Blindheim, Fredrik Heen; Ræder, Synnøve Brandt; Bjørnstad, Frithjof Andreas; Sundby, Eirik; Hoff, Bård Helge; Bruheim, Per; Otterlei, Marit (Peer reviewed; Journal article, 2023)The past few decades have been plagued by an increasing number of infections caused by antibiotic resistant bacteria. To mitigate the rise in untreatable infections, we need new antibiotics with novel targets and drug ... -
Adaptation of central metabolite pools to variations in growth rate and cultivation conditions in Saccharomyces cerevisiae
Kumar, Kanhaiya; Venkatraman, Vishwesh; Bruheim, Per (Peer reviewed; Journal article, 2021)Background Saccharomyces cerevisiae is a well-known popular model system for basic biological studies and serves as a host organism for the heterologous production of commercially interesting small molecules and proteins. ... -
Analyse av stressinduserte metabolske endringer i prostatakreftcellelinjen DU-145 ved bruk av massespektrometriske metoder
Jakobsen, Marte Aase (Master thesis, 2014)Det har blitt identifisert et nytt bindingssete på ''proliferating cell nuclear antigen'' (PCNA), kalt ''AlkB homologue 2 PCNA-interacting motif'' (APIM). Proteiner involvert i blant annet DNA-reparasjon og regulering av ... -
Analysis of DNA Repair Proteins and Chromatin Remodeling Factors Using iPOND
Søgaard, Caroline Danielsen (Master thesis, 2012)Most chemotherapeutic treatments rely on induction of severe DNA damage to kill the cancer cells. However, DNA repair pathways can repair the induced lesions, leading to survival of the tumor cells. Thus, inhibition of DNA ... -
Analysis of stress-induced metabolic response in UM-UC-3 cells exposed to the APIM-containing peptide drug ATX-101
Nøst, Renathe Haugdahl (Master thesis, 2016)The proliferating cell nuclear antigen (PCNA) is an essential scaffold protein central that is indispensable in several of the cellular repair processes through its numerous protein interaction partners. Under cellular ... -
Analysis of The Consequences of Oxygen Limitation on the Redox Balance and Overflow Metabolites in Escherichia coli BL21 During Recombinant Protein Production
Refsnes, Åse (Master thesis, 2023)Rekombinant proteinproduksjon er en stor industri globalt. E. coli er det vanligste vertsorganismen som brukes til produksjon av rekombinante proteiner. Oksygenbegrensning i bioreaktorer under produksjon av rekombinante ... -
Analysis of the Metabolic Response of Saccharomyces Cerevisiae to DNA Damaging Agents
Rey, Simon Scheel (Master thesis, 2011)Saccharomyces cerevisiae, commonly known as Baker s yeast, is a eukaryotic model organism widely used in biotechnology research. Its genome has a high degree of similarity to humans, and research done on S. cerevisiae can ... -
Analysis of the Stress-Induced Metabolic Response in JJN-3 Cells to Exposure to the APIM-Containing Peptide Drug ATX-101
Ringstad, Birgit Drejer (Master thesis, 2014)Cancer therapy with cytostatic drugs aims to damage the DNA and cause cell death. However, various forms of cancer are characterized by drug resistance, often resulting from increased DNA repair. Inhibiting the cells' ... -
Anti-Cancer Potential of Homemade Fresh Garlic Extract Is Related to Increased Endoplasmic Reticulum Stress
Petrovic, Voin; Nepal, Anala; Olaisen, Camilla; Bachke, Siri; Hira, Jonathan; Søgaard, Caroline Krogh; Røst, Lisa Marie; Misund, Kristine; Andreassen, Trygve; Melø, Torun Margareta; Bartosova, Zdenka; Bruheim, Per; Otterlei, Marit (Journal article; Peer reviewed, 2018)The use of garlic and garlic-based extracts has been linked to decreased incidence of cancer in epidemiological studies. Here we examine the molecular and cellular activities of a simple homemade ethanol-based garlic extract ... -
Antimicrobial peptides targeting the beta-clamp
Nepal, Anala (Doctoral theses at NTNU;2023:94, Doctoral thesis, 2023) -
An assessment of serial co-cultivation approach for generating novel Zymomonas mobilis strains
Fuchino, Katsuya; Bruheim, Per (Peer reviewed; Journal article, 2020)Objective The alphaproteobacterium Zymomonas mobilis is an efficient ethanol producer, and Z. mobilis-based biorefinery shows great potential for biofuel production. Serial co-cultivation is an emerging approach that ... -
Bioconversion of Heavy oil: Characterizations of Microbial potential to bioconvert Mariner Maureen-, Peregrino- and Bressay oil
Steinbakk, Sandra (Master thesis, 2011)70 % of world s oil reservoirs consist of heavy oil, and as the supply of conventional oil decreases, researchers are searching for new technologies to explore and enhance heavy oil recovery. One of the postulated technologies ... -
Biological and Chemical Stability of Various Preparations of Garlic Extracts
Stork, Eirin (Master thesis, 2018)The beneficial effects of garlic (Allium Sativum) on cancer and on the cardiovascular system, as well as direct anti-bacterial and anti-viral properties, has been confirmed by multiple research papers, where organosulfur ... -
Central carbon metabolite profiling reveals vector-associated differences in the recombinant protein production host Escherichia coli BL21
Garcia Calvo, Laura; Rane, Divyata Vilas; Everson, Nikalet; Humlebrekk, Sigurd Tømmerberg; Mathiassen, Lise Femanger; Mæhlum, Astfrid Helene Morka; Malmo, Jostein; Bruheim, Per (Peer reviewed; Journal article, 2023)The Gram-negative bacterium Escherichia coli is the most widely used host for recombinant protein production, both as an industrial expression platform and as a model system at laboratory scale. The recombinant protein ... -
Centre for Digital Life Norway - Annual report 2017
Johansen, Finn-Eirik; Brautaset, Trygve; Aune, Marie Hjelmseth; Patriksson, Alexandra; Falkenberg, Liv Eggset; Kleppe, Rune; Dick, Gunnar; Strand, Berit Løkensgard; Eijsink, Vincent; Røyne, Anja; Goksøyr, Anders; Svendsen, John Sigurd Mjøen; Fyhn, Marianne; Vik, Jon Olav; Wentzel, Alexander; Aksnes, Astrid; Bruheim, Per; Carlsen, Sven Magnus; Åm, Heidrun (Research report, 2018) -
Changes in Plasma Pyruvate and TCA Cycle Metabolites upon Increased Hepatic Fatty Acid Oxidation and Ketogenesis in Male Wistar Rats
Dankel, Simon Nitter; Kalleklev, Tine-Lise; Tungland, siri Lunde; Stafsnes, Marit Hallvardsdotter; Bruheim, Per; Aloysius, Thomas Aquinas; Lindquist, Carine; SKORVE, JON; Nygård, Ottar Kjell; Madsen, Lise; Bjørndal, Bodil; Sydnes, Magne Olav; Berge, Rolf Kristian (Peer reviewed; Journal article, 2023)Altered hepatic mitochondrial fatty acid β-oxidation and associated tricarboxylic acid (TCA) cycle activity contributes to lifestyle-related diseases, and circulating biomarkers reflecting these changes could have disease ...