• 3CAPS - A structural AP-site analogue as a tool to investigate DNA base excision repair 

      Schürmann, David; Scheidegger, Simon P.; Weber, Alain R.; Bjørås, Magnar; Leumann, Christian J.; Schär, Primo (Peer reviewed; Journal article, 2016)
      Abasic sites (AP-sites) are frequent DNA lesions, arising by spontaneous base hydrolysis or as intermediates of base excision repair (BER). The hemiacetal at the anomeric centre renders them chemically reactive, which ...
    • 8-oxoguanine DNA glycosylase (Ogg1) controls hepatic gluconeogenesis 

      Scheffler, Katja; Rachek, Lyudmila; You, Panpan; Rowe, Alexander D.; Wang, Wei; Kuśnierczyk, Anna; Kittelsen, Lene Svendsen; Bjørås, Magnar; Eide, Lars (Journal article; Peer reviewed, 2018)
      Mitochondrial DNA (mtDNA) resides in close proximity to metabolic reactions, and is maintained by the 8-oxoguanine DNA glycosylase (Ogg1) and other members of the base excision repair pathway. Here, we tested the hypothesis ...
    • A systems approach to study immuno- and neuro-modulatory properties of antiviral agents 

      Zusinaite, Eva; Ianevski, Alexandr; Niukkanen, Diana; Poranen, Minna M.; Bjørås, Magnar; Afset, Jan Egil; Tenson, Tanel; Velagapudi, Vidya; Merits, Andres; Kainov, Denis (Journal article; Peer reviewed, 2018)
      There are dozens of approved, investigational and experimental antiviral agents. Many of these agents cause serious side effects, which can only be revealed after drug administration. Identification of the side effects ...
    • A transgenic minipig model of Huntington’s disease shows early signs of behavioral and molecular pathologies 

      Askeland, Georgina; Rodinova, Marie; Štufková, Hana; Dosoudilova, Zaneta; Baxa, Monika; Smatlikova, Petra; Bohuslavova, Bozena; Klempir, Jiri; Nguyen, The Duong; Kuśnierczyk, Anna; Bjørås, Magnar; Klungland, Arne; Hansikova, Hana; Ellederova, Zdenka; Eide, Lars (Journal article; Peer reviewed, 2018)
      Huntington's disease (HD) is a monogenic, progressive, neurodegenerative disorder with currently no available treatment. The Libechov transgenic minipig model for HD (TgHD) displays neuroanatomical similarities to humans ...
    • Additive manufacturing of laminar flow cells for single-molecule experiments 

      Ahmadi, Arash; Till, Katharina; Hafting, Yngve; Schuttpelz, Mark; Bjørås, Magnar; Glette, Kyrre; Tørresen, Jim; Rowe, Alexander D.; Dalhus, Bjørn (Journal article; Peer reviewed, 2019)
      A microfluidic laminar flow cell (LFC) forms an indispensable component in single-molecule experiments, enabling different substances to be delivered directly to the point under observation and thereby tightly controlling ...
    • Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression 

      Montaldo, Nicola Pietro; Bordin, Diana Lilian; Brambilla, Alessandro; Rösinger, Marcel; Martin, Sarah Fordyce; Bjørås, Karine Øian; Bradamante, Stefano; Aas, Per Arne; Furrer, Antonia; Olsen, Lene Christin; Kunath, Nicolas; Otterlei, Marit; Sætrom, Pål; Bjørås, Magnar; Samson, Leona D.; van Loon, Barbara (Journal article; Peer reviewed, 2019)
      Base excision repair (BER) initiated by alkyladenine DNA glycosylase (AAG) is essential for removal of aberrantly methylated DNA bases. Genome instability and accumulation of aberrant bases accompany multiple diseases, ...
    • Common nodes of virus–host interaction revealed through an integrated network analysis 

      Bösl, Korbinian; Ianevski, Aleksandr; Than, Thoa T.; Andersen, Petter I.; Kuivanen, Suvi; Teppor, Mona; Zusinaite, Eva; Dumpis, Uga; Vitkauskiene, Astra; Cox, Rebecca Jane; Kallio-Kokko, Hannimari; Bergqvist, Anders; Tenson, Tanel; Merits, Andres; Oksenych, Valentyn; Bjørås, Magnar; Anthonsen, Marit Walbye; Shum, David H.K.; Kaarbø, Mari; Vapalahti, Olli; Windisch, Marc P.; Superti-Furga, Giulio; Snijder, Berend; Kainov, Denis; Kandasamy, Richard Kumaran (Journal article; Peer reviewed, 2019)
      Viruses are one of the major causes of acute and chronic infectious diseases and thus a major contributor to the global burden of disease. Several studies have shown how viruses have evolved to hijack basic cellular pathways ...
    • Complex alternative splicing of human Endonuclease V mRNA, but evidence for only a single protein isoform 

      Berges, Natalia; Nawaz, Meh Sameen; Dahl, Tuva Børresdatter; Hagen, Lars; Bjørås, Magnar; Lærdahl, Jon Kristen; Alseth, Ingrun (Journal article; Peer reviewed, 2019)
      Endonuclease V (ENDOV) is a ribonuclease with affinity for inosine which is the deamination product of adenosine. The genomes of most organisms, including human, encode ENDOV homologs, yet knowledge about in vivo functions ...
    • Development of a human cerebral organoid model for brain ischemia 

      Nestvold, Håkon Høydal (Master thesis, 2019)
      Å forstå de fundamentale prosessene i forbindelse med sykdom i menneskehjernene i dag, er vanskelig fordi vi mangler generalisering og overførbarhet av resultater fra eksisterende in vitro- og in vivo-modeller. Fokuset for ...
    • Diverse functions of DNA glycosylases processing oxidative base lesions in brain 

      Scheffler, Katja; Bjørås, Karine Øian; Bjørås, Magnar (Peer reviewed; Journal article, 2019)
      Endogenous and exogenous oxidative agents continuously damage genomic DNA, with the brain being particularly vulnerable. Thus, preserving genomic integrity is key for brain health and neuronal function. Accumulation of DNA ...
    • DNA glycosylase Neil2 contributes to genomic responses in the spleen during clinical prion disease 

      Scheffler, Katja; Jalland, Clara Maria Osnes; Benestad, Sylvie L.; Moldal, Torfinn; Ersdal, Cecilie; Gunnes, Gjermund; Suganthan, Rajikala; Bjørås, Magnar; Tranulis, Michael A. (Peer reviewed; Journal article, 2020)
      The DNA glycosylase Neil2 is a member of the base excision repair (BER) family of enzymes, which are important for repair of oxidative DNA damage. Specifically, Neil2 participates in repair of oxidized bases in single-stranded ...
    • Dynamics of base excision repair proteins; recruitment to replication forks and DNA lesions 

      Bjørås, Karine Øian (Doctoral theses at NTNU;2017:275, Doctoral thesis, 2017)
      DNA er arvestoffet vårt og finnes i de fleste celler i kroppen. DNA er satt sammen av to tråder bestående av sukkermolekyler, fosfat og nitrogenbaser (nukleotider). Disse kjemiske strukturene blir kontinuerlig utsatt for ...
    • Efficient and Reliable Production of Vectors for the Study of the Repair, Mutagenesis, and Phenotypic Consequences of Defined DNA Damage Lesions in Mammalian Cells 

      Petrova, Lucy; Neurauter, Christine Gran; Bjørås, Magnar; Doetsch, Paul W (Peer reviewed; Journal article, 2016)
      Mammalian cells are constantly and unavoidably exposed to DNA damage from endogenous and exogenous sources, frequently to the detriment of genomic integrity and biological function. Cells acquire a large number of chemically ...
    • Excision of the doubly methylated base N4,5-dimethylcytosine from DNA by Escherichia coli Nei and Fpg proteins 

      Alexeeva, Marina; Guragain, Prashanna; Tesfahun, Almaz Nigatu; Tomkuvienė, Miglė; Arshad, Aysha; Gerasimaitė, Rūta; Rukšėnaitė, Audronė; Urbanavičiūtė, Giedrė; Bjørås, Magnar; Lærdahl, Jon Kristen; Klungland, Arne; Klimašauskas, Saulius; Bjelland, Svein (Journal article; Peer reviewed, 2018)
      Cytosine (C) in DNA is often modified to 5-methylcytosine (m5C) to execute important cellular functions. Despite the significance of m5C for epigenetic regulation in mammals, damage to m5C has received little attention. ...
    • Exercise induces cerebral VEGF and angiogenesis via the lactate receptor HCAR1 

      Morland, Cecilie; Andersson, Krister; Haugen, Øyvind Pernell; Hadzic, Alena; Kleppa, Liv; Gille, Andreas; Rinholm, Johanne Egge; Palibrk, Vuk; Diget, Elisabeth Holm; Kennedy, Lauritz Hagen; Stølen, Tomas; Hennestad, Eivind; Moldestad, Olve; Cai, Yiqing; Puchades, Maja Amedjkouh; Offermanns, Stefan; Vervaeke, Koen Gerard Alois; Bjørås, Magnar; Wisløff, Ulrik; Storm-Mathisen, Jon; Bergersen, Linda Hildegard (Journal article; Peer reviewed, 2017)
      Physical exercise can improve brain function and delay neurodegeneration; however, the initial signal from muscle to brain is unknown. Here we show that the lactate receptor (HCAR1) is highly enriched in pial fibroblast-like ...
    • Generation of a Mouse Model Lacking the Non-Homologous End-Joining Factor Mri/Cyren 

      Castaneda Zegarra, Sergio Miguel; Huse, Camilla; Røsand, Øystein; Sarno, Antonio; Xing, Mengtan; Zhang, Qindong; Alirezaylavasani, Amin; Werner, Julia; Ji, Ping; Liabakk, Nina-Beate; Wang, Wei; Bjørås, Magnar; Oksenych, Valentyn (Journal article; Peer reviewed, 2019)
      Classical non-homologous end joining (NHEJ) is a molecular pathway that detects, processes, and ligates DNA double-strand breaks (DSBs) throughout the cell cycle. Mutations in several NHEJ genes result in neurological ...
    • Genetic interaction between the non-homologous end joining factors in mice and humans 

      Xing, Mengtan (Doctoral theses at NTNU;2019:117, Doctoral thesis, 2019)
    • Histone methylations define neural stem/progenitor cell subtypes in the mouse subventricular zone 

      Zhang, Zhichao; Manaf, Adeel; Li, Yanjiao; Pérez, Sonia Peña; Suganthan, Rajikala; Dahl, John Arne; Bjørås, Magnar; Klungland, Arne (Journal article; Peer reviewed, 2019)
      Neural stem/progenitor cells (NSPCs) persist in the mammalian brain throughout life and can be activated in response to the physiological and pathophysiological stimuli. Epigenetic reprogramming of NPSC represents a novel ...
    • Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability 

      Bosshard, Matthias; Aprigliano, Rossana; Gattiker, Cristina; Palibrk, Vuk; Markkanen, Enni; Backe, Paul Hoff; Pellegrino, Stefania; Raymond, F. Lucy; Froyen, Guy; Altmeyer, Matthias; Bjørås, Magnar; Dianov, Grigory L.; van Loon, Barbara (Journal article; Peer reviewed, 2017)
      Mutations in the HECT, UBA and WWE domain-containing 1 (HUWE1) E3 ubiquitin ligase cause neurodevelopmental disorder X-linked intellectual disability (XLID). HUWE1 regulates essential processes such as genome integrity ...
    • Increased nuclear DNA damage precedes mitochondrial dysfunction in peripheral blood mononuclear cells from Huntington's disease patients 

      Askeland, Georgina; Dosoudilova, Zaneta; Rodinova, Marie; Klempir, Jiri; Liskova, Irena; Kusnierczyk, Anna; Bjørås, Magnar; Nesse, Gaute; Klungland, Arne; Hansikova, Hana; Eide, Lars (Journal article; Peer reviewed, 2018)
      Huntington’s disease (HD) is a progressive neurodegenerative disorder primarily affecting the basal ganglia and is caused by expanded CAG repeats in the huntingtin gene. Except for CAG sizing, mitochondrial and nuclear DNA ...