Browsing NTNU Open by Author "Aachmann, Finn Lillelund"
Now showing items 1-20 of 44
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1H, 13C and 15N backbone and side-chain assignment of a carbohydrate binding module from a xylanase from Roseburia intestinalis
Madland, Eva; Kitaoku, Yoshihito; Sætrom, Gerd Inger; Leth, Maria Louise; Ejby, Morten; Hachem, Maher Abou; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2018)The N-terminal domain (residues 28–165) from the glycoside hydrolase family 10 from Roseburia intestinalis (RiCBMx), has been isotopically labeled and recombinantly expressed in Escherichia coli. Here we report 1H, 13C and ... -
1H, 13C, 15N resonance assignment of the chitin-active lytic polysaccharide monooxygenase BlLPMO10A from Bacillus licheniformis
Courtade, Gaston; Balzer, Simone; Forsberg, Zarah; Vaaje-Kolstad, Gustav; Eijsink, Vincent; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2015)The chitin-active 19.2 kDa lytic polysaccharide monooxygenase BlLPMO10A from Bacillus licheniformis has been isotopically labeled and recombinantly expressed. In this paper, we report the 1H, 13C, 15N resonance assignment ... -
A C4-oxidizing lytic polysaccharide monooxygenase cleaving both cellulose and cello-oligosaccharides
Isaksen, Trine; Westereng, Bjørge; Aachmann, Finn Lillelund; Agger, Jane; Kracher, Daniel; Kittl, Roman; Ludwig, R.; Haltrich, Dietmar; Eijsink, Vincent; Horn, Svein Jarle (Journal article; Peer reviewed, 2014)Lignocellulosic biomass is a renewable resource that significantly can substitute fossil resources for the production of fuels, chemicals, and materials. Efficient saccharification of this biomass to fermentable sugars ... -
A colorimetric assay to rapidly determine the activities of lytic polysaccharide monooxygenases
Wang, Damao; Li, Jing; Wong, Ann C. Y.; Aachmann, Finn Lillelund; Hsieh, Yves S. Y. (Journal article; Peer reviewed, 2018)Background Lytic polysaccharide monooxygenase (LPMOs) are enzymes that catalyze the breakdown of polysaccharides in biomass and have excellent potential for biorefinery applications. However, their activities are relatively ... -
A novel expression system for lytic polysaccharide monooxygenases
Courtade, Gaston; Le, Simone Balzer; Sætrom, Gerd Inger; Brautaset, Trygve; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2017)Lytic polysaccharide monooxygenases (LPMOs) are key enzymatic players of lignocellulosic biomass degradation processes. As such, they have been introduced in cellulolytic cocktails for more efficient and less expensive ... -
Analytical Tools for Characterizing Cellulose-Active Lytic Polysaccharide Monooxygenases (LPMOs)
Westereng, Bjørge; Loose, Jennifer Sarah Maria; Vaaje-Kolstad, Gustav; Aachmann, Finn Lillelund; Sørlie, Morten; Eijsink, Vincent (Journal article; Peer reviewed, 2018)Lytic polysaccharide monooxygenases are copper-dependent enzymes that perform oxidative cleavage of glycosidic bonds in cellulose and various other polysaccharides. LPMOs acting on cellulose use a reactive oxygen species ... -
Backbone 1H, 13C and 15N chemical shift assignment of full-length human uracil DNA glycosylase UNG2
Buchinger, Edith; Wiik, Siv Åshild; Kusnierczyk, Anna; Rabe, Renana; Aas, Per Arne; Kavli, Bodil Merete; Slupphaug, Geir; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2017)Human uracil N-glycosylase isoform 2—UNG2 consists of an N-terminal intrinsically disordered regulatory domain (UNG2 residues 1–92, 9.3 kDa) and a C-terminal structured catalytic domain (UNG2 residues 93–313, 25.1 kDa). ... -
Backbone and side-chain 1H, 13C, and 15N chemical shift assignments for the apo-form of the lytic polysaccharide monooxygenase NcLPMO9C
Courtade, Gaston; Wimmer, Reinhard; Dimarogona, Maria; Sandgren, Mats; Eijsink, Vincent; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2016)The apo-form of the 23.3 kDa catalytic domain of the AA9 family lytic polysaccharide monooxygenase NcLPMO9C from Neurospora crassa has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, ... -
Biosynthesis and Function of Long Guluronic Acid-Blocks in Alginate Produced by Azotobacter vinelandii
Aarstad, Olav Andreas; Stanisci, Annalucia; Sætrom, Gerd Inger; Tøndervik, Anne; Sletta, Håvard; Aachmann, Finn Lillelund; Skjåk-Bræk, Gudmund (Journal article; Peer reviewed, 2019)With the present accessibility of algal raw material, microbial alginates as a source for strong gelling material are evaluated as an alternative for advanced applications. Recently, we have shown that alginate from algal ... -
Chemical shift assignments for the apo-form of the catalytic domain, the linker region, and the carbohydrate-binding domain of the cellulose-active lytic polysaccharide monooxygenase ScLPMO10C
Courtade, Gaston; Forsberg, Zarah; Vaaje-Kolstad, Gustav; Eijsink, Vincentius Gerardus Henricus; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2017)The apo-form of the 21.4 kDa catalytic domain and the 10.7 kDa carbohydrate binding domain of the AA10 family lytic polysaccharide monooxygenase ScLPMO10C from Streptomyces coelicolor have been isotopically labeled and ... -
Chitosan as a wound dressing starting material: antimicrobial Properties and mode of action
Matica, Mariana Adina; Aachmann, Finn Lillelund; Tøndervik, Anne; Sletta, Håvard; Ostafe, Vasile (Journal article; Peer reviewed, 2019)Fighting bacterial resistance is one of the concerns in modern days, as antibiotics remain the main resource of bacterial control. Data shows that for every antibiotic developed, there is a microorganism that becomes ... -
Development of mannuronan C-5 epimerases to perform in vitro tailoring and upgrading of alginates
Stanisci, Annalucia (Doctoral theses at NTNU;2017:325, Doctoral thesis, 2017) -
Efficient functionalization of alginate biomaterials
Dalheim, Marianne Øksnes; Vanacker, Julie; Najmi, Maryam A.; Aachmann, Finn Lillelund; Strand, Berit Løkensgard; Christensen, Bjørn E. (Journal article; Peer reviewed, 2016)Peptide coupled alginates obtained by chemical functionalization of alginates are commonly used as scaffold materials for cells in regenerative medicine and tissue engineering. We here present an alternative to the commonly ... -
Efficient Grafting of Cyclodextrin to Alginate and Performance of the Hydrogel for Release of Model Drug
Omtvedt, Line Aanerud; Dalheim, Marianne Øksnes; Nielsen, Thorbjørn T.; Larsen, Kim; Strand, Berit Løkensgard; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2019)Controlling the rate of release of molecules from a hydrogel is of high interest for various drug delivery systems and medical devices. A strategy to alter the release profiles of soluble and poorly soluble active ingredients ... -
Energy Landscape of Alginate-Epimerase Interactions Assessed by Optical Tweezers and Atomic Force Microscopy
Håti, Armend Gazmeno; Aachmann, Finn Lillelund; Stokke, Bjørn Torger; Skjåk-Bræk, Gudmund; Sletmoen, Marit (Journal article, 2015)Mannuronan C-5 epimerases are a family of enzymes that catalyze epimerization of alginates at the polymer level. This group of enzymes thus enables the tailor-making of various alginate residue sequences to attain various ... -
Enzyme kinetics of mannuronan C-5 epimerase AlgE4 studied by 13C-NMR and CD spectroscopy
Børve, Marta (Master thesis, 2019)Alginater er en gruppe polysakkarider med et bredt anvendelsesområde. De har ulike fysiske egenskaper, som hovedsakelig bestemmes av sekvensensen til de to monomerne, alfa-D-mannuronat (M) og beta-L-guluronat (G). Sekvensen ... -
Extraction, Characterization, and Biological Activities of Laminarin and Fucoidan from Laminaria hyperborea
Henriksson, Emilia Linnea (Master thesis, 2019)Fucoidan og laminaran finnes som biprodukter i sidestrømmer som stammer fra alginatproduksjon. Utnyttelse av restråstoff fra tare kan generere produkter av kommersiell verdi, og dermed føre til økonomisk vekst for ... -
Functional characterization of three Azotobacter chroococcum alginate-modifying enzymes related to the Azotobacter vinelandii AlgE mannuronan C-5-epimerase family
Gawin, Agnieszka Maria; Tietze, Lisa; Aarstad, Olav Andreas; Aachmann, Finn Lillelund; Brautaset, Trygve; Ertesvåg, Helga (Peer reviewed; Journal article, 2020)Bacterial alginate initially consists of 1-4-linked beta-D-mannuronic acid residues (M) which can be later epimerized to alpha-L-guluronic acid (G). The family of AlgE mannuronan C-5-epimerases from Azotobacter vinelandii ... -
Genome Mining of Streptomyces sp. YIM 130001 Isolated From Lichen Affords New Thiopeptide Antibiotic
Schneider, Olha; Simic, Nebojsa; Aachmann, Finn Lillelund; Rückert, Christian; Kristiansen, Kåre Andre; Kalinowski, Jörn; Jiang, Yi; Wang, Lisong; Jiang, Cheng-Lin; Lale, Rahmi; Zotchev, Sergey B (Journal article; Peer reviewed, 2018)Streptomyces bacteria are recognized as an important source for antibiotics with broad applications in human medicine and animal health. Here, we report the isolation of a new lichen-associating Streptomyces sp. YIM 130001 ... -
Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity
Frigols, Belen; Marti, Miguel; Salesa, Beatriz; Hernandez-Oliver, Carolina; Aarstad, Olav Andreas; Ulset, Ann-Sissel Teialeret; Sætrom, Gerd Inger; Aachmann, Finn Lillelund; Serrano-Aroca, Angel (Peer reviewed; Journal article, 2019)Alginate is considered an exceptional biomaterial due to its hydrophilicity, biocompatibility, biodegradability, nontoxicity and low-cost in comparison with other biopolymers. We have recently demonstrated that the ...