Blar i NTNU Open på forfatter "Røst, Lisa Marie"
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Absolute Quantification of the Central Carbon Metabolome in Eight Commonly Applied Prokaryotic and Eukaryotic Model Systems
Røst, Lisa Marie; Thorfinnsdottir, Lilja Brekke; Kumar, Kanhaiya; Fuchino, Katsuya; Langørgen, Ida Eide; Bartosova, Zdenka; Kristiansen, Kåre Andre; Bruheim, Per (Peer reviewed; Journal article, 2020)Absolute quantification of intracellular metabolite pools is a prerequisite for modeling and in-depth biological interpretation of metabolomics data. It is the final step of an elaborate metabolomics workflow, with challenges ... -
Anti-Cancer Potential of Homemade Fresh Garlic Extract Is Related to Increased Endoplasmic Reticulum Stress
Petrovic, Voin; Nepal, Anala; Olaisen, Camilla; Bachke, Siri; Hira, Jonathan; Søgaard, Caroline Krogh; Røst, Lisa Marie; Misund, Kristine; Andreassen, Trygve; Melø, Torun Margareta; Bartosova, Zdenka; Bruheim, Per; Otterlei, Marit (Journal article; Peer reviewed, 2018)The use of garlic and garlic-based extracts has been linked to decreased incidence of cancer in epidemiological studies. Here we examine the molecular and cellular activities of a simple homemade ethanol-based garlic extract ... -
Effects of Hypoxia on Cell Growth and Metabolism in the Leukemia Cell Line JJN-3 and Bladder Cancer Cell Line UM-UC-3
Langørgen, Ida Eide (Master thesis, 2018)Hypoxia is a condition in which the oxygen (O2) access is extremely reduced and thus limits the O2 available for cellular respiration. The hypoxic condition induces metabolic adjustments in the cells that permit further ... -
ETNK1 mutations induce a mutator phenotype that can be reverted with phosphoethanolamine
Fontana, Diletta; Mauri, Mario; Renso, Rossella; Docci, Mattia; Crespiatico, Ilaria; Røst, Lisa Marie; Jang, Mi; Niro, Antonio; D’Aliberti, Deborah; Massimino, Luca; Bertagna, Mayla; Zambrotta, Giovanni; Bossi, Mario; Citterio, Stefania; Crescenzi, Barbara; Fanelli, Francesca; Cassina, Valeria; Corti, Roberta; Salerno, Domenico; Nardo, Luca; Chinello, Clizia; Mantegazza, Francesco; Mecucci, Cristina; Magni, Fulvio; Cavaletti, Guido; Bruheim, Per; Larsen, Steen; Rea, Delphine; Gambacorti-Passerini, Carlo; Piazza, Rocco (Peer reviewed; Journal article, 2020)Recurrent somatic mutations in ETNK1 (Ethanolamine-Kinase-1) were identified in several myeloid malignancies and are responsible for a reduced enzymatic activity. Here, we demonstrate in primary leukemic cells and in cell ... -
Exploring the Metabolic Adaptions to Hypoxia and the Role of mTOR and Hsp90 Inhibition in Hypoxia and Normoxia in Multiple Myeloma
Dahl, Anna Bergan (Master thesis, 2020)Hypoksi defineres som en tilstand der oksygennivået er redusert sammenlignet med det fysiologiske nivået. Ondartede kreftsvulster har ofte hypoksiske områder, fordi deres ukontrollerte proliferering og mangelfulle ... -
Improved phosphometabolome profiling applying isotope dilution strategy and capillary ion chromatography-tandem mass spectrometry
Stafsnes, Marit Hallvardsdotter; Røst, Lisa Marie; Bruheim, Per (Journal article; Peer reviewed, 2018)The phosphometabolome is comprised of all phosphorylated metabolites including the major metabolite classes sugar phosphates and nucleoside phosphates. Phosphometabolites are invaluable in any cell as a part of primary- ... -
Mass Spectrometric Metabolic Profiling of Bladder Cancer Cell Line T24 under Cisplatin and APIM-peptide Stress
Fjørtoft, Marit Otterlei (Master thesis, 2016)The hub protein proliferating cell nuclear antigen (PCNA) is involved in several important cellular processes. During conditions of genotoxic stress, PCNA binds to proteins containing AlkB homologue 2 PCNA-interacting motif ... -
Mass Spectrometric Metabolic Profiling of the Human Bladder Cancer Cell Line UM-UC-3 under Cytostatic Stress
Forsland, Yngve (Master thesis, 2016)Muscle Invasive Bladder Cancers (MIBCs) contributes to approximately 30% of bladder cancer at initial diagnosis in patients, accompanied by an only 50% five-year survival rate. The standard procedure for treating this type ... -
Mass Spectrometric Metabolic Profiling of the Multiple Myeloma Cell Line JJN-3 under APIM-peptide Stress
Røst, Lisa Marie (Master thesis, 2015)The proliferating cell nuclear antigen (PCNA) is recognized as a hub protein, central to maintaining cellular homeostasis through its numerous protein interaction partners. Under conditions of genotoxic stress, there is ... -
Mass Spectrometric Metabolic Profiling of the Prostate Cancer Cell Line DU-145 and the Glioblastoma Astrocytoma Cell Line U-87 MG Under Hypoxic Stress
Brinch, Ulrikke Dahl (Master thesis, 2017)Hypoxia is a condition of mild oxygen (O2) deficiency and is observed in most solid tumors. It is believed to trigger malignant transformation, alter metabolism, and it contributes to, for example, metastasis, invasiveness, ... -
Metabolic Profiling of Cisplatin and ATX-101 Induced Stress-responses in the Bladder Cancer Cell Line T24
Olsen, Silje Malene (Master thesis, 2016)Proliferating cell nuclear antigen (PCNA) is a protein central in many cellular processes through its numerous protein interaction partners. Recently, it has been shown that several proteins involved in stress related ... -
Metabolic Profiling of the Glioblastoma Cell Line U-87 MG in Response to Hypoxia and HIF-1alpha Inhibitors
Zhevagin, Irina Brokke (Master thesis, 2020)Glioblastom er en krefttype med høy dødelighet, og dens dødelighet har sammenheng med graden av hypoksi. Hypoksi, fraværet av nok oksygenering av vev, vil aktivere en rekke tilpasninger som fører til økning i behandlingsresistens ... -
Metabolic Responses to Colistin Sulfate and Trimethoprim Perturbation in Escherichia coli K12 MG1655
Evensen, Nora Østli (Master thesis, 2022)Antibiotika er antibakterielle molekyler som brukes i behandling mot bakterieinfeksjoner, men grunnet overforbruk og feilbruk har antibiotikaresistens blitt et økende problem. Antibiotikaresistens regnes som en av de største ... -
Metabolsk Tilpasning til Hypoksi i Prostatakreftcellelinjen DU-145 og i Glioblastoma-Astrocytoma Kreftcellelinjen U-87 MG
Midtbust, Elise (Master thesis, 2017)Regioner med lav oxygentensjon (hypoksiske regioner) er karakteristisk for solide tumorer. Hypoksiske forhold fremkommer når den raske cellulære prolifereringen begrenser oksygentilgangen. Proteinkomplekset proliferating ... -
Phosphatase of Regenerating Liver-3 Regulates Cancer Cell Metabolism in Multiple Myeloma
Abdollahi, Pegah; Vandsemb, Esten; Elsaadi, Samah; Røst, Lisa Marie; Yang, Rui; Hjort, Magnus Aassved; Andreassen, Trygve; Misund, Kristine; Slørdahl, Tobias Schmidt; Rø, Torstein Baade; Sponaas, Anne-Marit; Moestue, Siver Andreas; Bruheim, Per; Børset, Magne (Peer reviewed; Journal article, 2021)Cancer cells often depend on microenvironment signals from molecules such as cytokines for proliferation and metabolic adaptations. PRL‐3, a cytokine‐induced oncogenic phosphatase, is highly expressed in multiple myeloma ... -
Transcriptomic and Metabolomic Analysis of the Escherichia coli Stress Response to Antibiotics With Different Modes of Action
Teigset, Erlend Gløer Stedje (Master thesis, 2022)Antibiotikaresistens er en eksistensiell trussel mot menneskeheten. Kampen mot antimikrobiell resistens krever både utvikling av nye behandlinger og forbedring av eksisterende behandlinger for å gjøre bakterier mer sårbare ... -
"Two hits - one stone" increased efficacy of cisplatin-based therapies by targeting PCNA's role in both DNA repair and cellular signaling
Søgaard, Caroline Krogh; Blindheim, Augun; Røst, Lisa Marie; Petrovic, Voin; Nepal, Anala; Bachke, Siri; Liabakk, Nina-Beate; Gederaas, Odrun Arna; Viset, Trond; Arum, Carl-Jørgen; Bruheim, Per; Otterlei, Marit (Journal article; Peer reviewed, 2018)Low response rate and rapid development of resistance against commonly used chemotherapeutic regimes demand new multi-targeting anti-cancer strategies. In this study, we target the stress-related roles of the scaffold ... -
Zwitterionic HILIC tandem mass spectrometry with isotope dilution for rapid, sensitive and robust quantification of pyridine nucleotides in biological extracts
Røst, Lisa Marie; Shafaei, Armaghan; Fuchino, Katsuya; Bruheim, Per (Peer reviewed; Journal article, 2020)The pyridine nucleotides nicotineamide adenine dinucleotide (NAD) and nicotineamide adenine dinucleotide phosphate (NADP) are conserved coenzymes across all domains of life, and are involved in more than 200 different ...