Blar i NTNU Open på forfatter "Valla, Svein"
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Regulation of the expression level of transcription factor XylS reveals new functional insight into its induction mechanism at the Pm promoter
Zwick, Friederike; Lale, Rahmi; Valla, Svein (Journal article; Peer reviewed, 2013)Background XylS is the positive regulator of the inducible Pm promoter, originating from Pseudomonas putida, where the system controls a biochemical pathway involved in degradation of aromatic hydrocarbons, which also act ... -
RpoS controls the expression and the transport of the AlgE1-7 epimerases in Azotobacter vinelandii
Moreno, Soledad; Ertesvåg, Helga; Valla, Svein; Nunez, C; Espín, Guadalupe; Cocotl-Yanez, Miguel (Journal article; Peer reviewed, 2018)Azotobacter vinelandii produces differentiated cells, called cysts, surrounded by two alginate layers, which are necessary for their desiccation resistance. This alginate contains variable proportions of guluronate residues, ... -
Safety in numbers: multiple occurrences of highly similar homologs among Azotobacter vinelandii carbohydrate metabolism proteins probably confer adaptive benefits
Mærk, Mali; Johansen, Jostein; Ertesvåg, Helga; Drabløs, Finn; Valla, Svein (Journal article; Peer reviewed, 2014)Background Gene duplication and horizontal gene transfer are common processes in bacterial and archaeal genomes, and are generally assumed to result in either diversification or loss of the redundant gene copies. However, ... -
Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette
Zwick, Friederike; Lale, Rahmi; Valla, Svein (Journal article; Peer reviewed, 2012)The XylS/Pm expression system has been used to produce recombinant proteins at industrial levels in Escherichia coli. Activation of transcription from the Pm promoter takes place in the presence of benzoic acid or derivatives ... -
Structural and functional characterization of the R-modules in alginate C-5 epimerases AlgE4 and AlgE6 from Azotobacter vinelandii
Buchinger, Edith; Knudsen, Daniel H.; Behrens, Manja A.; Pedersen, Jan Skov; Aarstad, Olav Andreas; Tøndervik, Anne; Valla, Svein; Skjåk-Bræk, Gudmund; Wimmer, Reinhard; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2014)The bacterium Azotobacter vinelandii produces a family of seven secreted and calcium-dependent mannuronan C-5 epimerases (AlgE1–7). These epimerases are responsible for the epimerization of β-d-mannuronic acid (M) to ... -
The B7-H3 Protein and its role in Breast Cancer Treatment Response
Pedersen, Cathrine (Master thesis, 2012)Breast cancer is the most common cancer type amongst women, and closer to 3000 women in Norway will be diagnosed with this disease in 2012. Although major improvements have been achieved in the treatment, and thus the ... -
The use of N-terminal peptide fusions to stimulate recombinant gene expression in Escherichia coli
Leithaug, Magnus (Master thesis, 2011)Previous studies by the Microbiology and Molecular Genetics (MOLGEN) research group at NTNU have investigated how N-terminal peptide fusions influence gene expression levels. The present study seeks to expand these efforts ... -
The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering.
Gawin, Agnieszka; Valla, Svein; Brautaset, Trygve (Journal article; Peer reviewed, 2017)The XylS/Pm regulator/promoter system originating from the Pseudomonas putida TOL plasmid pWW0 is widely used for regulated low- and high-level recombinant expression of genes and gene clusters in Escherichia coli and other ...