Browsing NTNU Open by Author "Skjåk-Bræk, Gudmund"
Now showing items 21-39 of 39
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Mn-alginate gels as a novel system for controlled release of Mn2+ in manganese-enhanced MRI
Mørch, Yrr Asbjørg; Sandvig, Ioanna; Olsen, Øystein; Donati, Ivan; Thuen, Marte; Skjåk-Bræk, Gudmund; Haraldseth, Olav; Brekken, Christian (Journal article; Peer reviewed, 2012)The aim of the present study was to test alginate gels of different compositions as a system for controlled release of manganese ions (Mn2+) for application in manganese‐enhanced MRI (MEMRI), in order to circumvent the ... -
Modification and Characterization of the Catalytic Subunit, the A-module, of the Mannuronan C-5 Epimerases AlgE4 and AlgE6
Dypås, Lene Brattsti (Master thesis, 2015)Alginate is a family of polysaccharides composed of (1 to 4)-linked beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G), with a wide variety of applications and even greater prospects for the future. Alginate is first ... -
Nanopartikler og inflammasjon
Olsen, Linda Therese (Master thesis, 2013)Nano- and submicron particles, inflammation and asthma Inflammation is an underlying condition of many diseases that represent important public health problems ranging from e.g. asthma to cancer. Particle exposure affects ... -
NMR structure of a lytic polysaccharide monooxygenase provides insight into copper binding, protein dynamics, and substrate interactions
Aachmann, Finn Lillelund; Sørlie, Morten; Skjåk-Bræk, Gudmund; Eijsink, Vincent; Vaaje-Kolstad, Gustav (Journal article; Peer reviewed, 2012)Lytic polysaccharide monooxygenases currently classified as carbohydrate binding module family 33 (CBM33) and glycoside hydrolase family 61 (GH61) are likely to play important roles in future biorefining. However, the ... -
Optimalised Carbodiimide Chemistry for RGD-coupled Alginate
McDonagh, Birgitte Hjelmeland (Master thesis, 2012)Alginate is a naturally ocurring polyanion of (1→4)linked β-d-mannuronic acid (M)and its C-5 epimer α-l guluronic acid (G). The polyanion, and particularly longstretches of guluronic acids, chelates and form hydrogels in ... -
Polymer sequencing by molecular machines: a framework for predicting the resolving power of a sliding contact force spectroscopy sequencing method
Dunlop, Alex; Bowman, Kate; Aarstad, Olav Andreas; Skjåk-Bræk, Gudmund; Stokke, Bjørn Torger; Round, Andrew N (Journal article, 2017)We evaluate an AFM-based single molecule force spectroscopy method for mapping sequences in otherwise difficult to sequence heteropolymers, including glycosylated proteins and glycans. The sliding contact force spectroscopy ... -
Rensing og karakterisering av mannuronan C-5-epimeraser produsert rekombinant i Hansenula polymorpha og mutante epimeraser produsert rekombinant i Escherichia coli
Rogstad, Henriette (Master thesis, 2012)Alginat er et polysakkarid som kommersielt utvinnes fra algekilder. Anvendelsesområder for alginat er blant annet mat, farmasi og biomedisin. Strukturen til alginat er avhengig av kilden den er ekstrahert fra, og komposisjonen ... -
Specialized Alginates and Microcapsules for Cell Therapy and Tissue Engineering: Focus on Immunological Properties in a Whole Blood Model
Hoem, Kine Samset (Master thesis, 2013)Alginates are a group of abundant natural polysaccharides with excellent gel-forming capacity and good biocompatibility, provided they are sufficiently well purified. Alginate shows great promise as an encapsulation material ... -
Strain Construction and Process Development for Efficient Recombinant Production of Mannuronan C-5 Epimerases in Hansenula polymorpha
Tøndervik, Anne; Aune, Randi; Degelmann, Adelheid; Piontek, Michael; Ertesvåg, Helga; Skjåk-Bræk, Gudmund; Sletta, Håvard (Peer reviewed; Journal article, 2022)Alginates are linear polysaccharides produced by brown algae and some bacteria and are composed of β-D-mannuronic acid (M) and α-L-guluronic acid (G). Alginate has numerous present and potential future applications within ... -
Structural and functional characterization of the R-modules in alginate C-5 epimerases AlgE4 and AlgE6 from Azotobacter vinelandii
Buchinger, Edith; Knudsen, Daniel H.; Behrens, Manja A.; Pedersen, Jan Skov; Aarstad, Olav Andreas; Tøndervik, Anne; Valla, Svein; Skjåk-Bræk, Gudmund; Wimmer, Reinhard; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2014)The bacterium Azotobacter vinelandii produces a family of seven secreted and calcium-dependent mannuronan C-5 epimerases (AlgE1–7). These epimerases are responsible for the epimerization of β-d-mannuronic acid (M) to ... -
Structure–Activity Relationships of Low Molecular Weight Alginate Oligosaccharide Therapy against Pseudomonas aeruginosa
Pritchard, Manon F.; Powell, Lydia C.; Adams, Jennifer Y. M.; Menzies, Georgina; Khan, Saira; Tøndervik, Anne; Sletta, Håvard; Aarstad, Olav Andreas; Skjåk-Bræk, Gudmund; McKenna, Stephen; Buurma, Niklaas J.; Farnell, Damian J. J.; Rye, Philip D.; Hill, Katja E.; Thomas, David W. (Peer reviewed; Journal article, 2023)Low molecular weight alginate oligosaccharides have been shown to exhibit anti-microbial activity against a range of multi-drug resistant bacteria, including Pseudomonas aeruginosa. Previous studies suggested that the ... -
Study of Rat Olfactory Ensheathing Cells in Alginate based Matrices
Fjelldal, Marthe Fredheim (Master thesis, 2012)Alginate hydrogel made from alginate and crosslinking divalent ions is a natural biomaterial that is biocompatible, has low toxicity, is relatively cheap and has mild gelation chemistry. It is a porous material that allows ... -
Submicron Particles and Inflammation
Mihaylova, Dessislava Dimitrova (Master thesis, 2012)Iron nanoparticles occur naturally in the environment, but their exposure increases dramatically due to the field of nanotechnology and –medicine. It is poorly understood how the intracellular cooperative mechanisms of ... -
Sulfated alginate microspheres associate with factor H and dampen the inflammatorycytokine response
Arlov, Øystein; Skjåk-Bræk, Gudmund; Rokstad, Anne Mari (Journal article; Peer reviewed, 2016)Alginate microspheres show promise for cell-encapsulation therapy but encounter challenges related to biocompatibility. In the present work we designed novel microbeads and microcapsules based on sulfated polyalternating ... -
Sulfated Alginates as Heparin Analogues: A Review of Chemical and Functional Properties
Arlov, Øystein; Skjåk-Bræk, Gudmund (Journal article; Peer reviewed, 2017)Heparin is widely recognized for its potent anticoagulating effects, but has an additional wide range of biological properties due to its high negative charge and heterogeneous molecular structure. This heterogeneity has ... -
SULFATED ALGINATES: Characterization and applications as biomaterials
Arlov, Øystein (Doctoral thesis at NTNU;2016:159, Doctoral thesis, 2016)As natural constituents of the extracellular matrix, sulfated glycosaminoglycans (GAGs) are of great interest in the design of novel biomaterials for cell immobilization and tissue engineering. A substantial challenge ... -
Tailoring of alginate gel properties with mannuronan C-5 epimerases: Correlations between structural and physical properties
Berg, Johan Robert (Master thesis, 2013)Alginates unique ability to form gels with divalent ions is one of the major properties for its use in food, pharmaceutics and medicine. The physical properties of ionic crosslinked alginate gels are highly connected to ... -
The role of bone morphogenetic protein-9 in multiple myeloma
Olsen, Oddrun Elise (Master thesis, 2012)Multiple myeloma (MM) is a malignancy of antibody producing plasma cells located in the bone marrow. MM is also called bone marrow cancer and is the second most common hematologic cancer. The exact cause for this type of ... -
The Role of Complement in Coagulation Activation Induced by Alginate Microspheres in Human Whole Blood
Hamad, Shamal Ahmad (Master thesis, 2013)Diabetes mellitus (DM) is serious disease that affects millions of people worldwide.Type 1 Diabetes (T1D) is an autoimmune disease, caused by destruction of the insulin producing beta cells (ß-cells) of the pancreas. ...