Blar i NTNU Open på forfatter "Lale, Rahmi"
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Genome Mining of Streptomyces sp. YIM 130001 Isolated From Lichen Affords New Thiopeptide Antibiotic
Schneider, Olha; Simic, Nebojsa; Aachmann, Finn Lillelund; Rückert, Christian; Kristiansen, Kåre Andre; Kalinowski, Jörn; Jiang, Yi; Wang, Lisong; Jiang, Cheng-Lin; Lale, Rahmi; Zotchev, Sergey B (Journal article; Peer reviewed, 2018)Streptomyces bacteria are recognized as an important source for antibiotics with broad applications in human medicine and animal health. Here, we report the isolation of a new lichen-associating Streptomyces sp. YIM 130001 ... -
Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens
Tietze, Lisa; Hoff, Maria Wilhelmsen; Lale, Rahmi (Peer reviewed; Journal article, 2022)Vibrio natriegens has recently gained attention as a novel fast-growing bacterium in synthetic biology applications. Currently, a limited set of genetic elements optimised for Escherichia coli are used in V. natriegens due ... -
Importance of the 5′ regulatory region to bacterial synthetic biology applications
Tietze, Lisa; Lale, Rahmi (Journal article; Peer reviewed, 2021) -
In vitro studies of DNA condensation by bridging protein in a crowding environment
Ramisetty, Sravani Keerthi; Langlete, Petter; Lale, Rahmi; Dias, Rita de Sousa (Journal article; Peer reviewed, 2017)The macromolecules of the bacterial cell occupy 20–40% of the total cytosol volume, and crowded environments have long been known to compact and stabilize DNA. Nevertheless, investigations on DNA-protein binding are generally ... -
"Insights into codon usage bias and development of synthetic biology tools for advancing gene and pathway expression in microorganisms"
Fages-Lartaud, Maxime (Doctoral theses at NTNU;2022:64, Doctoral thesis, 2022)The thesis explores the feasibility of recoding genomes in algae to expand the genetic code to non-canonical amino acids. The thesis investigates the features of the chloroplast’s genetic code to understand how it can be ... -
Investigating the binding of Escherichia coli Sigma Factor 54 (σ54) to specific DNA promoter sequences through In vitro bulk Transcription.
Javed, Arsal (Master thesis, 2022)Prosjektet tar sikte på å undersøke transkripsjonsreguleringen i Escherichia coli ved σ54. Prokaryote promotere er kjent for å ha UP-elementer og en kjernepromoterregion som er plassert oppstrøms for den kodende sekvensen ... -
Investigation of Pilin Function in the Cyanobacterium Synechocystis sp. PCC 6803
Bøe, Benedikte (Master thesis, 2014)The aim of this project was to investigate the involvement of a specific operon from Synechocystis sp. PCC 6803 (hereafter Synechocystis 6803) in iron acquisition. The operon selected consists of the four genes, slr1928, ... -
Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity
Shojaei Moghadam, Morteza; Albersmeier, Andreas; Winkler, Anika; Cimmino, Lorenzo; Rise, Kjersti; Hohmann-Marriott, Martin Frank; Kalinowski, Jörn; Rückert, Christian; Wentzel, Alexander; Lale, Rahmi (Journal article; Peer reviewed, 2016)Background Marine cold-temperature environments are an invaluable source of psychrophilic microbial life for new biodiscoveries. An Arctic marine bacterial strain collection was established consisting of 1448 individual ... -
mCherry contains a fluorescent protein isoform that interferes with its reporter function
Fages-Lartaud, Maxime René; Tietze, Lisa; Elie, Florence; Lale, Rahmi; Hohmann-Marriott, Martin Frank (Peer reviewed; Journal article, 2022)Fluorescent proteins are essential reporters in cell and molecular biology. Here, we found that red-fluorescent proteins possess an alternative translation initiation site that produces a short functional protein isoform ... -
Microscale tools for the development of bacterial microarrays
Arnfinnsdottir, Nina Bjørk (Doctoral thesis at NTNU;2016:61, Doctoral thesis, 2016)Heterogeneity within bacterial populations is a phenomenon that has gained much interest over the last decades. It has been shown that even isogeneous colonies under homogeneous conditions can display different phenotypes. ... -
A novel 5' UTR design for improved gene/protein expression in Escherichia coli - Genetic construction, phenotypic characterization and transcript amount analysis of dualUTR constructs
Onsager, Ingerid (Master thesis, 2017)The 5' untranslated region of bacterial mRNA (UTR) play an important role in mRNA translation. It is a crucial contributor to the maintenance of a balance between transcription, transcript stability and translation. ... -
Oil degrading potentials of cold-loving (psychrophilic) bacteria
Cimmino, Lorenzo (Master thesis, 2015)A collection of 1400 psychrophilic isolates, belonging to 31 different genera, has been screened with the intent to identify the strains that harbor the ability to degrade polycyclic aromatic hydrocarbons (PAHs). The 16S ... -
Optimizing biomass formation of riboflavin producing C. glutamicum strains growing on SSL sugars using Model Predictive Control
Kallman, Dina Riis (Master thesis, 2020)Riboflavin, eller vitamin B2 er et essensielt mellomledd for alle plante- og dyreceller. Det er industrielt viktig innen helse, mat og i dyrefôrindustrien. Corynebacterium glutamicum har blitt mye brukt i industriell ... -
Psychrobacter spp. from an Arctic marine environment: Versatile candidates for the degradation of phenolic compounds
Shojaei-Moghadam, Morteza (Doctoral thesis at NTNU;2016:133, Doctoral thesis, 2016)Phenol and its derivatives have challenged pollution/waste management due to being structurally recalcitrant to degradation processes. Bio-based processes continue to be the preferred choice for removal of phenols due to ... -
Regulation of Fibroblast Growth Factor Receptor (FGFR) Signaling
Irion, Karen (Master thesis, 2024)Fibroblast vekstfaktor reseptorer (FGFRer) er signalerings reseptorer som regulerer cellebiologiske prosesser som proliferering og migrering. Overaktivering av FGFRer er knyttet til flere ulike kreftformer. Aktivering av ... -
Regulation of the expression level of transcription factor XylS reveals new functional insight into its induction mechanism at the Pm promoter
Zwick, Friederike; Lale, Rahmi; Valla, Svein (Journal article; Peer reviewed, 2013)Background XylS is the positive regulator of the inducible Pm promoter, originating from Pseudomonas putida, where the system controls a biochemical pathway involved in degradation of aromatic hydrocarbons, which also act ... -
Reproducibility of fluorescent expression from engineered biological constructs in E. coli.
Beal, J, et al.; Lale, Rahmi (Peer reviewed; Journal article, 2016)We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions ... -
Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002
Vogel, Anne Ilse Maria; Lale, Rahmi; Hohmann-Marriott, Martin Frank (Journal article; Peer reviewed, 2017)Background: Synechococcus sp. PCC 7002 (henceforth Synechococcus) is developing into a powerful synthetic biology chassis. In order to streamline the integration of genes into the Synechococcus chromosome, validation of ... -
Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette
Zwick, Friederike; Lale, Rahmi; Valla, Svein (Journal article; Peer reviewed, 2012)The XylS/Pm expression system has been used to produce recombinant proteins at industrial levels in Escherichia coli. Activation of transcription from the Pm promoter takes place in the presence of benzoic acid or derivatives ... -
The Design of Simple Bacterial Microarrays: Development towards Immobilizing Single Living Bacteria on Predefined Micro-Sized Spots on Patterned Surfaces
Arnfinnsdottir, Nina Bjørk; Ottesen, Vegar; Lale, Rahmi; Sletmoen, Marit (Journal article; Peer reviewed, 2015)In this paper we demonstrate a procedure for preparing bacterial arrays that is fast, easy, and applicable in a standard molecular biology laboratory. Microcontact printing is used to deposit chemicals promoting bacterial ...