Blar i NTNU Open på forfatter "Rokstad, Anne Mari"
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Alginate microbeads are coagulation compatible, while alginate microcapsules activate coagulation secondary to complement or directly through FXII
Gravastrand, Caroline; Hamad, Shamal; Fure, Hilde; Steinkjer, Bjørg; Ryan, Liv; Oberholzer, José; Lambris, John D.; Lacík, Igor; Mollnes, Tom Eirik; Brekke, Ole-Lars; Rokstad, Anne Mari (Journal article; Peer reviewed, 2017)Alginate microspheres are presently under evaluation for future cell-based therapy. Their ability to induce harmful host reactions needs to be identified for developing the most suitable devices and efficient prevention ... -
Alginate microbeads are complement compatible, in contrast to polycation containing microcapsules, as revealed in a human whole blood model
Rokstad, Anne Mari; Brekke, Ole Lars; Steinkjer, Bjørg; Ryan, Liv; Kolláriková, Gabriela; Strand, Berit Løkensgard; Skjåk-Bræk, Gudmund; Lacík, Igor; Espevik, Terje; Mollnes, Tom Eirik (Journal article; Peer reviewed, 2011)Alginate microbeads and microcapsules are presently under evaluation for future cell-based therapy. Defining their inflammatory properties with regard to humans is therefore essential. A lepirudine-based human whole blood ... -
Alginate Microcapsules for Cell Therapy: Effect of capsule composition on complement activation, cytokine secretion, and protein adsorption in a whole blood model
Ørning, Mathias Pontus Andreas (Master thesis, 2012)Encapsulation of pancreatic islets in alginate microbeads and microcapsules show great promise for the treatment of Type 1 diabetes mellitus. Significant progress has been made in developing a biocompatible capsule that ... -
Alginate Microencapsulation of Human Islets Does Not Increase Susceptibility to Acute Hypoxia
Hals, Ingrid Katrin; Rokstad, Anne Mari; Strand, Berit Løkensgard; Oberholzer, José; Grill, Valdemar (Journal article; Peer reviewed, 2013)Islet transplantation in diabetes is hampered by the need of life-long immunosuppression. Encapsulation provides partial immunoprotection but could possibly limit oxygen supply, a factor that may enhance hypoxia-induced ... -
Alginate microsphere compositions dictate different mechanisms of complement activation with consequences for cytokine release and leukocyte activation
Ørning, Mathias Pontus; Hoem, Kine Samseth; Coron, Abba Elizabeth; Skjåk-Bræk, Gudmund; Mollnes, Tom Eirik; Brekke, Ole Lars; Espevik, Terje; Rokstad, Anne Mari (Journal article; Peer reviewed, 2016)The inflammatory potential of 12 types of alginate-based microspheres was assessed in a human whole blood model. The inflammatory potential could be categorized from low to high based on the four main alginate microsphere ... -
Biocompatibility and Biotolerability Assessment of Microspheres Using a Whole Blood Model
Rokstad, Anne Mari; Strand, Berit Løkensgard; Espevik, Terje; Mollnes, Tom Eirik (Journal article, 2013)The whole blood model is a powerful method to determine the immediate inflammatory reactions towards foreign objects in general. This review focuses on the use of a lepirudin based whole blood model for evaluating microspheres ... -
Cholesterol Crystals Induce coagulation Activation through Complement-Dependent Expression of Monocytic Tissue Factor
Gravastrand, Caroline S.; Steinkjer, Bjørg; Halvorsen, Bente; Landsem, Anne; Skjelland, Mona; Jacobsen, Eva Astrid; Woodruff, Trent M.; Lambris, John D.; Mollnes, Tom Eirik; Brekke, Ole-Lars; Espevik, Terje; Rokstad, Anne Mari (Journal article; Peer reviewed, 2019)Cholesterol crystals (CC) are strong activators of complement and could potentially be involved in thromboinflammation through complement-coagulation cross-talk. To explore the coagulation-inducing potential of CC, we ... -
Explorations of in vitro models for new understanding of the host-material response
Kjærnsmo, Fredrikke (Master thesis, 2020)Celleinnkapslingteknologi benytter biomaterialer for transplantasjon av celler og viser potensiale som et terapeutisk alternativ til behandling av diabetes type 1. Fagfeltet har kommet langt i utviklingen av kapsler for ... -
In Vitro and In Vivo Biocompatibility Evaluation of Polyallylamine and Macromolecular Heparin Conjugates Modified Alginate Microbeads
Vaithilingam, Vijayaganapathy; Steinkjer, Bjørg; Ryan, Liv; Larsson, Rolf; Tuch, Bernard E.; Oberholzer, José; Rokstad, Anne Mari (Journal article; Peer reviewed, 2017)Host reactivity to biocompatible immunoisolation devices is a major challenge for cellular therapies, and a human screening model would be of great value. We designed new types of surface modified barium alginate microspheres, ... -
Iron oxide nanoparticles induce cytokine secretion in a complement-dependent manner in a human whole blood model
Wolf-Grosse, Susann; Rokstad, Anne Mari; Ali, Syed; Lambris, JD; Mollnes, Tom Eirik; Nilsen, Asbjørn Magne; Stenvik, Jørgen (Journal article; Peer reviewed, 2017)Abstract: Iron oxide nanoparticles (IONPs) are promising nanomaterials for biomedical applications. However, their inflammatory potential has not been fully established. Here, we used a lepirudin anti-coagulated human whole ... -
Microencapsulation of small intestinal neuroendocrine neoplasm cells for tumor model studies
Rokstad, Anne Mari; Gustafsson, Björn; Espevik, Terje; Bakke, Ingunn; Pfragner, Roswitha; Svejda, Bernhard; Modlin, Irvin M; Kidd, Mark (Journal article; Peer reviewed, 2012)Basic cancer research is dependent on reliable in vitro and in vivo tumor models. The serotonin (5‐HT) producing small intestinal neuroendocrine tumor cell line KRJ‐1 has been used in in vitro proliferation and secretion ... -
Obesity weight reduction and inflammatory leukocyte receptor expression : expression of toll-like receptors and inflammation-linked receptors
Teigen, Ellen (Master thesis, 2016)Obesity is defined as accumulation and storage of excessive body fat, and is increasing worldwide. Obesity is associated with diseases as diabetes mellitus type 2 and cardiovascular diseases. A hallmark of obesity is chronic ... -
Producing ultrapure wood cellulose nanofibrils and evaluating the cytotoxicity using human skin cells
Nordli, Henriette R.; Chinga-Carrasco, Gary; Rokstad, Anne Mari; Pukstad, Brita (Journal article; Peer reviewed, 2016)Wood cellulose nanofibrils (CNF) have been suggested as a potential wound healing material, but its utilization is limited by FDA requirements regarding endotoxin levels. In this study a method using sodium hydroxide ... -
Reconstituted High-Density Lipoprotein Attenuates Cholesterol Crystal-Induced Inflammatory Responses by Reducing Complement Activation
Niyonzima, Nathalie; Samstad, Eivind; Aune, Marie Hjelmseth; Ryan, Liv; Bakke, Siril Skaret; Rokstad, Anne Mari; Wright, Samuel; Damås, Jan Kristian; Mollnes, Tom Eirik; Latz, Eicke; Espevik, Terje (Journal article; Peer reviewed, 2015)Chronic inflammation of the arterial wall is a key element in the development of atherosclerosis, and cholesterol crystals (CC) that accumulate in plaques are associated with initiation and progression of the disease. We ... -
Specialized Alginates and Microcapsules for Cell Therapy and Tissue Engineering: Focus on Immunological Properties in a Whole Blood Model
Hoem, Kine Samset (Master thesis, 2013)Alginates are a group of abundant natural polysaccharides with excellent gel-forming capacity and good biocompatibility, provided they are sufficiently well purified. Alginate shows great promise as an encapsulation material ... -
Sulfated alginate microspheres associate with factor H and dampen the inflammatorycytokine response
Arlov, Øystein; Skjåk-Bræk, Gudmund; Rokstad, Anne Mari (Journal article; Peer reviewed, 2016)Alginate microspheres show promise for cell-encapsulation therapy but encounter challenges related to biocompatibility. In the present work we designed novel microbeads and microcapsules based on sulfated polyalternating ... -
The Role of Complement in Coagulation Activation Induced by Alginate Microspheres in Human Whole Blood
Hamad, Shamal Ahmad (Master thesis, 2013)Diabetes mellitus (DM) is serious disease that affects millions of people worldwide.Type 1 Diabetes (T1D) is an autoimmune disease, caused by destruction of the insulin producing beta cells (ß-cells) of the pancreas. ... -
Ultrapure Wood Nanocellulose - Assessments of Coagulation and Initial Inflammation Potential
Nordli, Henriette R.; Pukstad, Brita; Chinga-Carrasco, Gary; Rokstad, Anne Mari (Journal article; Peer reviewed, 2019)Using a lepirudin-based human whole blood model, we evaluated the initial inflammatory and coagulation responses of dense and porous ultrapure (<50 endotoxin units/grams) cellulose nanofibrils (CNF), of carboxylated grade. ...