Browsing NTNU Open by Author "Sharma, Amit Kumar"
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A CRISPR/Cas9 system adapted for gene editing in marine algae
Sparstad, Torfinn; Sharma, Amit Kumar; Bones, Atle M.; Nymark, Marianne; Winge, Per (Journal article; Peer reviewed, 2016)Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens ... -
Design and optimization of a crossflow tube reactor system for hydrogen production by combining ethanol steam reforming and water gas shift reaction
Chen, Wei-Hsin; Lu, Chen-Yu; Chou, Wei-Shan; Sharma, Amit Kumar; Ayyadurai, Saravanakumar; Tran, Khanh-Quang (Peer reviewed; Journal article, 2022)Crossflow tube reactors with crossflow configuration are considered a special design for hydrogen production via ethanol steam reforming with less catalyst than conventional packed bed reactors. However, the results showed ... -
Genome editing of marine algae: Technology development and use of the CRISPR/Cas9 system for studies of light harvesting complexes and regulation of phosphate homeostasis
Sharma, Amit Kumar (Doctoral theses at NTNU;2020:160, Doctoral thesis, 2020) -
Loss of CpFTSY Reduces Photosynthetic Performance and Affects Insertion of PsaC of PSI in Diatoms
Nymark, Marianne; Finazzi, Giovanni; Volpe, Charlotte; Serif, Manuel Philipp; Fonseca, Davi de Miranda; Sharma, Animesh; Sanchez Puerto, Nicolas; Sharma, Amit Kumar; Ashcroft, Felicity Jayne; Kissen, Ralph; Winge, Per; Bones, Atle Magnar (Peer reviewed; Journal article, 2023)The chloroplast signal recognition particle (CpSRP) receptor (CpFTSY) is a component of the CpSRP pathway that post-translationally targets light-harvesting complex proteins (LHCPs) to the thylakoid membranes in plants and ... -
Simultaneous knockout of multiple LHCF genes using single sgRNAs and engineering of a high-fidelity Cas9 for precise genome editing in marine algae
Sharma, Amit Kumar; Nymark, Marianne; Flo, Snorre; Sparstad, Torfinn; Bones, Atle M.; Winge, Per (Peer reviewed; Journal article, 2021)The CRISPR/Cas9 system is an RNA-guided sequence-specific genome editing tool, which has been adopted for single or multiple gene editing in a wide range of organisms. When working with gene families with functional ... -
The Myb like transcription factor Phosphorus Starvation Response (PtPSR) controls conditional P acquisition and remodeling in marine microalgae
Sharma, Amit Kumar; Mühlroth, Alice; Jouhet, Juliette; Maréchal, Eric; Alipanah, Leila; Kissen, Ralph; Brembu, Tore; Bones, Atle M.; Winge, Per (Journal article; Peer reviewed, 2019)Phosphorus (P) is one of the limiting macronutrients for algal growth in marine environments. Microalgae have developed adaption mechanisms to P limitation that involve remodelling of internal phosphate resources and ... -
Transgene-free genome editing in marine algae by bacterial conjugation - comparison with biolistic CRISPR/Cas9 transformation
Sharma, Amit Kumar; Nymark, Marianne; Sparstad, Torfinn; Bones, Atle M.; Winge, Per (Journal article; Peer reviewed, 2018)The CRISPR/Cas9 technology has opened the possibility for targeted genome editing in various organisms including diatom model organisms. One standard method for delivery of vectors to diatom cells is by biolistic particle ...