Browsing NTNU Open by Author "Courtade, Gaston"
Now showing items 1-20 of 24
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1H, 13C, 15N resonance assignment of the apo form of the small, chitin-active lytic polysaccharide monooxygenase JdLPMO10Afrom Jonesia denitrificans
Christensen, Idd Andrea; Eijsink, Vincent; Aachmann, Finn Lillelund; Courtade, Gaston (Peer reviewed; Journal article, 2020)The lytic polysaccharide monooxygenase JdLPMO10A is the N-terminal domain of the multimodular protein Jd1381. The isolated JdLPMO10A domain is one of the smallest chitin-active lytic polysaccharide monooxygenases known to ... -
1H, 13C, 15N resonance assignment of the chitin-active lytic polysaccharide monooxygenase BlLPMO10A from Bacillus licheniformis
Courtade, Gaston; Balzer, Simone; Forsberg, Zarah; Vaaje-Kolstad, Gustav; Eijsink, Vincent; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2015)The chitin-active 19.2 kDa lytic polysaccharide monooxygenase BlLPMO10A from Bacillus licheniformis has been isotopically labeled and recombinantly expressed. In this paper, we report the 1H, 13C, 15N resonance assignment ... -
A novel expression system for lytic polysaccharide monooxygenases
Courtade, Gaston; Le, Simone Balzer; Sætrom, Gerd Inger; Brautaset, Trygve; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2017)Lytic polysaccharide monooxygenases (LPMOs) are key enzymatic players of lignocellulosic biomass degradation processes. As such, they have been introduced in cellulolytic cocktails for more efficient and less expensive ... -
An NMR investigation of lytic polysaccharide monooxygenases
Courtade, Gaston (Doctoral theses;2018:337, Doctoral thesis, 2018)Bioeconomy requires sustainable sources of biomass (e.g. chitin and cellulose) to use as energy, feed and biomaterials. In the quest of biomass conversion, lytic polysaccharide monooxygenases (LPMOs) are key enzymatic ... -
Backbone and side-chain 1H, 13C, and 15N chemical shift assignments for the apo-form of the lytic polysaccharide monooxygenase NcLPMO9C
Courtade, Gaston; Wimmer, Reinhard; Dimarogona, Maria; Sandgren, Mats; Eijsink, Vincent; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2016)The apo-form of the 23.3 kDa catalytic domain of the AA9 family lytic polysaccharide monooxygenase NcLPMO9C from Neurospora crassa has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, ... -
Chemical shift assignments for the apo-form of the catalytic domain, the linker region, and the carbohydrate-binding domain of the cellulose-active lytic polysaccharide monooxygenase ScLPMO10C
Courtade, Gaston; Forsberg, Zarah; Vaaje-Kolstad, Gustav; Eijsink, Vincentius Gerardus Henricus; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2017)The apo-form of the 21.4 kDa catalytic domain and the 10.7 kDa carbohydrate binding domain of the AA10 family lytic polysaccharide monooxygenase ScLPMO10C from Streptomyces coelicolor have been isotopically labeled and ... -
Class IV lasso peptides synergistically induce proliferation of cancer cells and sensitize them to doxorubicin
Madland, Eva; Felipe Guerrero-Garzón, Jaime; Zehl, Martin; Singh, Madhurendra; Rezaei, Shiva; Aachmann, Finn Lillelund; Courtade, Gaston; Urban, Ernst; Rückert, Christian; Busche, Tobias; Kalinowski, Jörn; Cao, Yan-Ru; Jiang, Yi; Jiang, Cheng-lin; Selivanova, Galina; Zotchev, Sergey (Peer reviewed; Journal article, 2020)Heterologous expression of a biosynthesis gene cluster from Amycolatopsis sp. resulted in the discovery of two unique class IV lasso peptides, felipeptins A1 and A2. A mixture of felipeptins stimulated proliferation of ... -
Comparative characterization of biotechnologically modified xanthan
Johansen, Kristoffer (Master thesis, 2023)Xanthan er et allsidig bakterielt ekstracellulært polysakkarid (EPS) som brukes i mange ulike bransjer på grunn av sine unike fysiske egenskaper. Modifisering av egenskapene til xanthan kan føre til nye bruksområder av ... -
The effect of linker conformation on performance and stability of a two-domain lytic polysaccharide monooxygenase
Forsberg, Zarah; Stepnov, Anton; Tesei, Giulio; Wang, Yong; Buchinger, Edith; Kristiansen, Sandra; Aachmann, Finn Lillelund; Arleth, Lise; Eijsink, Vincent; Lindorff-Larsen, Kresten; Courtade, Gaston (Journal article; Peer reviewed, 2023)A considerable number of lytic polysaccharide monooxygenases (LPMOs) and other carbohydrate-active enzymes are modular, with catalytic domains being tethered to additional domains, such as carbohydrate-binding modules, by ... -
Enzyme kinetics of mannuronan C-5 epimerase AlgE4 studied by 13C-NMR and CD spectroscopy
Børve, Marta (Master thesis, 2019)Alginater er en gruppe polysakkarider med et bredt anvendelsesområde. De har ulike fysiske egenskaper, som hovedsakelig bestemmes av sekvensensen til de to monomerne, alfa-D-mannuronat (M) og beta-L-guluronat (G). Sekvensen ... -
Functional characterization of a lytic polysaccharide monooxygenase from Schizophyllum commune that degrades non-crystalline substrates
Østby, Heidi; Christensen, Idd Andrea; Hennum, Karen; Varnai, Aniko; Buchinger, Edith; Grandal, Siri; Courtade, Gaston; Hegnar, Olav Aaseth; Aachmann, Finn Lillelund; Eijsink, Vincent (Journal article; Peer reviewed, 2023)Lytic polysaccharide monooxygenases (LPMOs) are mono-copper enzymes that use O2 or H2O2 to oxidatively cleave glycosidic bonds. LPMOs are prevalent in nature, and the functional variation among these enzymes is a topic of ... -
Human Chitotriosidase: Catalytic Domain or Carbohydrate Binding Module, Who’s Leading HCHT’s Biological Function
Crasson, Oscar; Courtade, Gaston; Leonard, Raphael R; Aachmann, Finn Lillelund; Legrand, Francois; Parente, Raffaella; Baurain, Denis; Galleni, Moreno; Sørlie, Morten; Vandevenne, Marylene (Journal article; Peer reviewed, 2017)Chitin is an important structural component of numerous fungal pathogens and parasitic nematodes. The human macrophage chitotriosidase (HCHT) is a chitinase that hydrolyses glycosidic bonds between the N-acetyl-D-glucosamine ... -
Interactions of a fungal lytic polysaccharide monooxygenase with β-glucan substrates and cellobiose dehydrogenase
Courtade, Gaston; Wimmer, Reinhard; Kjendseth, Åsmund Røhr; Preims, Marita; Felice, Alfons K.G.; Dimarogona, Maria; Vaaje-Kolstad, Gustav; Sørlie, Morten; Sandgren, Mats; Ludwig, Roland; Eijsink, Vincent; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2016)Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that catalyze oxidative cleavage of glycosidic bonds using molecular oxygen and an external electron donor. We have used NMR and isothermal titration ... -
Investigations into Lytic Polysaccharide Monooxygenase functionalities using NMR
Christensen, Idd Andrea (Doctoral theses at NTNU;2024:25, Doctoral thesis, 2024)English summary Lytic Polysaccharide Monooxygenases (LPMOs) are enigmatic enzymes that oxidatively depolymerize crystalline polysaccharides like cellulose and chitin. Following their discovery in 2010, LPMOs have gained ... -
Mechanistic basis of substrate–O2 coupling within a chitin-active lytic polysaccharide monooxygenase: An integrated NMR/EPR study
Courtade, Gaston; Ciano, Luisa; Paradisi, Alessandro; Lindley, Peter J.; Forsberg, Zarah; Sørlie, Morten; Wimmer, Reinhard; Davies, Gideon J.; Eijsink, Vincent; Walton, Paul H.; Aachmann, Finn Lillelund (Peer reviewed; Journal article, 2020)Lytic polysaccharide monooxygenases (LPMOs) have a unique ability to activate molecular oxygen for subsequent oxidative cleavage of glycosidic bonds. To provide insight into the mode of action of these industrially important ... -
Microbial Exopolysaccharides: Evaluation of Glycosyltransferase GumM and 13C Isotope-Labeling of Bacterial Cellulose
Lyngstad, Hera Karlsen (Master thesis, 2021)Mikrobielle eksopolysakkarider er en mangfoldig underkategori av ekstracellulære polymeriske stoffer som utskilles av mikroorganismer. Disse forbindelsene har som regel høy molekylvekt og består av monomerer som er ... -
On the impact of carbohydrate-binding modules (CBMs) in lytic polysaccharide monooxygenases (LPMOs)
Forsberg, Zarah; Courtade, Gaston (Peer reviewed; Journal article, 2022)Lytic polysaccharide monooxygenases (LPMOs) have revolutionized our understanding of how enzymes degrade insoluble polysaccharides. Compared with the substantial knowledge developed on the structure and mode of action of ... -
Polysaccharide degradation by lytic polysaccharide monooxygenases
Forsberg, Zarah; Sørlie, Morten; Petrovic, Dejan; Courtade, Gaston; Aachmann, Finn Lillelund; Vaaje-Kolstad, Gustav; Bissaro, Bastien; Kjendseth, Åsmund Røhr; Eijsink, Vincent (Journal article; Peer reviewed, 2019)The discovery of oxidative cleavage of glycosidic bonds by enzymes currently known as lytic polysaccharide monooxygenases (LPMOs) has had a major impact on our current understanding of the enzymatic conversion of recalcitrant ... -
Recombinant expression and structural investigation of JdLPMO10A using Nuclear magnetic Resonance (NMR) spectroscopy
(Master thesis, 2019)En bærekraftig produksjon av karbonnøytral energi er ekstremt viktig dersom verdenssamfunnet skal oppnå 2,0 graders målet satt ved Parisavtalen i 2015. Et stort fokus har derfor blitt lagt på utviklingen av fornybare ... -
Resonance assignments for the apo-form of the cellulose-active lytic polysaccharide monooxygenase TaLPMO9A
Kitaoku, Yoshihito; Courtade, Gaston; Petrovic, Dejan; Fukamizo, Tamo; Eijsink, Vincent; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2018)The apo-form of the 24.4 kDa AA9 family lytic polysaccharide monooxygenase TaLPMO9A from Thermoascus aurantiacus has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, we report the ...