Browsing NTNU Open by Author "Bones, Atle M."
Now showing items 1-20 of 109
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A CRISPR/Cas13-based method for downregulation of ALB3 genes in Phaeodactylum tricornutum
Mironenko Castelló, Guillermo (Master thesis, 2021)Diatoms, a group of unicellular photosynthetic microalgae, are of high ecological importance. They are responsible for about 20% of the world’s oxygen production. They have also been exploited in research due to their high ... -
A CRISPR/Cas9 system adapted for gene editing in marine algae
Sparstad, Torfinn; Sharma, Amit Kumar; Bones, Atle M.; Nymark, Marianne; Winge, Per (Journal article; Peer reviewed, 2016)Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens ... -
A mixture model approach to sample size estimation in two- sample comparative microarray experiments
Jørstad, Tommy Stokmo; Midelfart, Herman; Bones, Atle M. (Journal article; Peer reviewed, 2008)Background: Choosing the appropriate sample size is an important step in the design of a microarray experiment, and recently methods have been proposed that estimate sample sizes for control of the False Discovery Rate ... -
A sex-inducing pheromone triggers cell cycle arrest and mate attraction in the diatom Seminavis robusta
Moeys, Sara; Frenkel, Johannes; Lembke, Christian; Gillard, Jeroen; Devos, Valerie; Van den Berge, Koen; Bouillon, Barbara; Huysman, Marie JJ; De Decker, Sam; Scharf, Julia; Bones, Atle M.; Brembu, Tore; Winge, Per; Sabbe, Koen; Vuylsteke, Martin; Clement, Lieven; De Veylder, Lieven; Pohnert, Georg; Vyverman, Wim (Journal article; Peer reviewed, 2016)Although sexual reproduction is believed to play a major role in the high diversification rates and species richness of diatoms, a mechanistic understanding of diatom life cycle control is virtually lacking. Diatom sexual ... -
Accumulation of (Ag(I) by Saccharomyces cereviseae cells expressing plant metallothioneins
Ruta, Lavinia L; Banu, Melania A.; Neagoe, Aurora D; Kissen, Ralph; Bones, Atle M.; Farcasanu, Ileana C. (Journal article; Peer reviewed, 2018)The various applications of Ag(I) generated the necessity to obtain Ag(I)-accumulating organisms for the removal of surplus Ag(I) from contaminated sites or for the concentration of Ag(I) from Ag(I)-poor environments. In ... -
Allyl isothiocyanate affects the cell cycle of Arabidopsis thaliana
Åsberg, Signe Elisabeth; Bones, Atle M.; Øverby, Anders (Journal article; Peer reviewed, 2015-05-19)Isothiocyanates (ITCs) are degradation products of glucosinolates present in members of the Brassicaceae family acting as herbivore repellents and antimicrobial compounds. Recent results indicate that allyl ITC (AITC) has ... -
Allyl isothiocyanate depletes glutathione and upregulates expression of glutathione S-transferases in Arabidopsis thaliana
Øverby, Anders; Stokland, Ragni Adelsten; Åsberg, Signe Elisabeth; Sporsheim, Bjørnar; Bones, Atle M. (Journal article; Peer reviewed, 2015-04-22)Allyl isothiocyanate (AITC) is a phytochemical associated with plant defense in plants from the Brassicaceae family. AITC has long been recognized as a countermeasure against external threats, but recent reports suggest ... -
Allyl isothiocyanate induces a shift in the cell cycle distribution of Arabidopsis thaliana
Åsberg, Signe Elisabeth (Master thesis, 2013)Isothiocyanates (ITCs) are degradation products of glucosinolates present in members of the Brassicaceae familiy acting e.g. as herbivore repellents and antimicrobial compounds. Recent results indicate that allyl ITC (AITC) ... -
Allyl isothiocyanate inhibits actin mediated intracellular transport in Arabidopsis thaliana.
Sporsheim, Bjørnar; Øverby, Anders; Bones, Atle M. (Journal article; Peer reviewed, 2015-12-07)Volatile allyl isothiocyanate (AITC) derives from the biodegradation of the glucosinolate sinigrin and has been associated with growth inhibition in several plants, including the model plant Arabidopsis thaliana. However, ... -
Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana
Kissen, Ralph; Øverby, Anders; Winge, Per; Bones, Atle M. (Journal article; Peer reviewed, 2016)Background Isothiocyanates (ITCs) are degradation products of the plant secondary metabolites glucosinolates (GSLs) and are known to affect human health as well as plant herbivores and pathogens. To investigate the processes ... -
Altering the Glucosinolate Profile of Arabidopsis thaliana: Characterization and Impact on Resistance Against Botrytis cinerea
Hanssen, Vilde Wiig (Master thesis, 2017)The glucosinolate-myrosinase system is a well-known part of the Brassicacea plant immunity. This family includes several important crop species as well as the model organism Arabidopsis thaliana, thus encouraging and ... -
An integrated analysis of molecular acclimation to high light in the marine diatom Phaeodactylum tricornutum
Nymark, Marianne; Valle, Kristin Collier; Brembu, Tore; Winge, Per; Hancke, Kasper; Andresen, Kjersti; Johnsen, Geir; Bones, Atle M. (Journal article; Peer reviewed, 2009-11-03)Photosynthetic diatoms are exposed to rapid and unpredictable changes in irradiance and spectral quality, and must be able to acclimate their light harvesting systems to varying light conditions. Molecular mechanisms behind ... -
Anchoring plant metallothioneins to the inner face of the plasma membrane of Saccharomyces cerevisiae cells leads to heavy metal accumulation
Ruta, Lavinia Liliana; Lin, Ya-Fen; Kissen, Ralph; Nicolau, Ioana; Neagoe, Aurora Daniela; Ghenea, Simona; Bones, Atle M.; Farcasanu, Ileana Cornelia (Journal article; Peer reviewed, 2017)In this study we engineered yeast cells armed for heavy metal accumulation by targeting plant metallothioneins to the inner face of the yeast plasma membrane. Metallothioneins (MTs) are cysteine-rich proteins involved in ... -
Arabidopsis mutants impaired in glutathione biosynthesis exhibit higher sensitivity towards the glucosinolate hydrolysis product allyl-isothiocyanate
Urbancsok, Janos; Bones, Atle M.; Kissen, Ralph (Journal article; Peer reviewed, 2018)Upon tissue damage the plant secondary metabolites glucosinolates can generate various hydrolysis products, including isothiocyanates (ITCs). Their role in plant defence against insects and pest and their potential health ... -
Arabidopsis myrosinases link the glucosinolate-myrosinase system and the cuticle.
Ahuja, Ishita; de Vos, RCH; Rohloff, Jens; Stoopen, G; Halle, Kari Krizak; Ahmad, SJN; Hoang, Linh; Hall, RD; Bones, Atle M. (Journal article; Peer reviewed, 2016)Both physical barriers and reactive phytochemicals represent two important components of a plant’s defence system against environmental stress. However, these two defence systems have generally been studied independently. ... -
Arabidopsis thaliana MIRO1 and MIRO2 GTPases Are Unequally Redundant in Pollen Tube Growth and Fusion of Polar Nuclei during Female Gametogenesis
Sørmo, Christopher Gravningen; Brembu, Tore; Winge, Per; Bones, Atle M. (Journal article; Peer reviewed, 2011)MIRO GTPases have evolved to regulate mitochondrial trafficking and morphology in eukaryotic organisms. A previous study showed that T-DNA insertion in the Arabidopsis MIRO1 gene is lethal during embryogenesis and affects ... -
Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination - Polyphenol oxidase (PPOA) as biomarker
Moradi, Babak; Kissen, Ralph; Maivan, Hassan Zare; Hashtroudi, Mehri Seyed; Sorahinobar, Mona; Sparstad, Torfinn; Bones, Atle M. (Peer reviewed; Journal article, 2020)Mangrove plants, which inhabit and form sensitive ecosystems in the intertidal zones of tropical and subtropical coastlines, though vulnerable to petroleum pollution, still maintain their growth under oil contamination. ... -
Assessment of oxidative stress response genes in Avicennia marina exposed to oil contamination – Polyphenol oxidase (PPOA) as a biomarker
Moradi, Babak; Kissen, Ralph; Maivan, Hassan Zare; Hashtroudi, Mehri Seyed; Sorahinobar, Mona; Sparstad, Torfinn; Bones, Atle M. (Journal article; Peer reviewed, 2020) -
Benzyl Cyanide Leads to Auxin-Like Effects Through the Action of Nitrilases in Arabidopsis thaliana
Urbancsok, Janos; Bones, Atle M.; Kissen, Ralph (Journal article; Peer reviewed, 2018)Plants within the Brassicales order generate glucosinolate hydrolysis products that can exert different biological effects on several organisms. Here, we evaluated the physiological effects of one of these compounds, benzyl ... -
Biokonvertering av tungolje kombinert med løsemiddelekstraksjon
Jonsen, Karen Leiråmo (Master thesis, 2011)Bitumen er en svært viskøs råolje som er vanskelig å utvinne. Selv om verdens tungoljereserver er store er det utvunnet svært lite tungolje ved bruk av tradisjonelle utvinningsmetoder. Behovet for drivstoff øker behovet ...