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Nuclear Magnetic Resonance spectroscopy of marine microalgae: Metabolic profiling and species discrimination from High-Resolution Magic Angle Spinning NMR analysis of whole-cell samples

Chauton, Matilde Skogen
Doctoral thesis
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URI
http://hdl.handle.net/11250/244502
Date
2005
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  • Fakultet for naturvitenskap (uspesifisert) [77]
Abstract
Based on what is said about the background and structure, this thesis presents a discussion of the use of NMR spectroscopy as a tool for species discrimination and metabolic profiling of microalgae. The results of various analyses including NMR and statistics are evaluated from what is known from already established identification methods such as pigment chromatography and chemotaxonomy, and the chemical composition of microalgal cells. The main focus is on the application of HR MAS on whole cells, which means quick and easy sample preparation with no extraction involved and large information output in terms of metabolite composition in a single analysis run.
Has parts
Chauton, MS; Tilstone, GH; Legrand, C; Johnsen, G. Changes in pigmentation, bio-optical characteristics and photophysiology, during phytoflagellate succession in mesocosms. Journal of Plankton Research. 26(3): 315-324, 2003.

Chauton, MS; Optun, OI; Bathen, TF; Volent, Z; Gribbestad, IS; Johnsen, G. HR MAS 'H NMR spectroscopy analysis of whole cells of marine microalgea. Marine Ecology Progress Series . 256: 57-62, 2003.

Chauton, MS; Bathen, TF. Metabolic profiling of microalgea whole cells with HR MAS'H NMR and principal component analysis. .

Chauton, MS; Størseth, TR; Johnsen, G. High-resolution magic angle spinning 1H NMR analysis of whole cells of Thalassiosira pseudonana (Bacillariophyceae): Broad range analysis of metabolic composition and nutritional value. Journal of Applied Phycology. 15(6): 533-542, 2003.

Chauton, MS; Størseth, TR; Krane, J. HIGH-RESOLUTION MAGIC ANGLE SPINNING NMR ANALYSIS OF WHOLE CELLS OF CHAETOCEROS MUELLERI (BACILLARIOPHYCEAE) AND COMPARISON WITH 13C-NMR AND DISTORTIONLESS ENHANCEMENT BY POLARIZATION TRANSFER 13C-NMR ANALYSIS OF LIPOPHILIC EXTRACTS. Journal of Phycology . 40(3): 611, 2004.
Publisher
Fakultet for naturvitenskap og teknologi
Series
Doktoravhandlinger ved NTNU, 1503-8181; 2005:13

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