Browsing NTNU Open by Author "Wentzel, Alexander"
Now showing items 1-20 of 23
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Assembly and application of genome-scale metabolic models to study Streptomyces coelicolor and Prochlorococcus
Sulheim, Snorre (Doctoral theses at NTNU;2021:96, Doctoral thesis, 2021)Summary: Metabolism is the set of all chemical reactions responsible for the conversion of nutrients into the energy and cellular building blocks required for growth and cellular maintenance in a living organism. Because ... -
Automatic reconstruction of metabolic pathways from identified biosynthetic gene clusters
Sulheim, Snorre; Fossheim, Fredrik A.; Wentzel, Alexander; Almaas, Eivind (Peer reviewed; Journal article, 2021)Background A wide range of bioactive compounds is produced by enzymes and enzymatic complexes encoded in biosynthetic gene clusters (BGCs). These BGCs can be identified and functionally annotated based on their DNA ... -
Biochemical Characterizations of Enzymes from Oil Reservoir Metagenomes
Hesthammer, Ronja Kristine (Master thesis, 2014)Microorganisms have evolved to exist in diverse environments on earth, even under the harsh conditions of oil reservoirs several kilometres below the seafloor. The environmental conditions there are extreme in several ... -
Biochemical Characterizations of Enzymes from Oil Reservoir Metagenomes
Hesthammer, Ronja Kristine (Master thesis, 2014)Microorganisms have evolved to exist in diverse environments on earth, even under the harsh conditions of oil reservoirs several kilometres below the seafloor. The environmental conditions there are extreme in several ... -
Butanol production from lignocellulosic biomass: revisiting fermentation performance indicators with exploratory data analysis
Birgen, Cansu; Dürre, Peter; Preisig, Heinz A.; Wentzel, Alexander (Journal article; Peer reviewed, 2019)After just more than 100 years of history of industrial acetone–butanol–ethanol (ABE) fermentation, patented by Weizmann in the UK in 1915, butanol is again today considered a promising biofuel alternative based on several ... -
Butanol production from lignocellulosic sugars by Clostridium beijerinckii in microbioreactors
Birgen, Cansu; Degnes, Kristin Fløgstad; Markussen, Sidsel; Wentzel, Alexander; Sletta, Håvard (Peer reviewed; Journal article, 2021)Butanol (n-butanol) has been gaining attention as a renewable energy carrier and an alternative biofuel with superior properties to the most widely used ethanol. We performed 48 anaerobic fermentations simultaneously with ... -
Centre for Digital Life Norway - Annual report 2017
Johansen, Finn-Eirik; Brautaset, Trygve; Aune, Marie Hjelmseth; Patriksson, Alexandra; Falkenberg, Liv Eggset; Kleppe, Rune; Dick, Gunnar; Strand, Berit Løkensgard; Eijsink, Vincent; Røyne, Anja; Goksøyr, Anders; Svendsen, John Sigurd Mjøen; Fyhn, Marianne; Vik, Jon Olav; Wentzel, Alexander; Aksnes, Astrid; Bruheim, Per; Carlsen, Sven Magnus; Åm, Heidrun (Research report, 2018) -
Efficacy of a novel sequential enzymatic hydrolysis of lignocellulosic biomass and inhibition characteristics of monosugars
Baksi, Sibashish; Ball, Akash K.; Sarkar, Ujjaini; Banerjee, Debopam; Wentzel, Alexander; Preisig, Heinz A.; Kuniyal, Jagdish Chandra; Birgen, Cansu; Saha, Sudeshna; Wittgens, Bernd; Markussen, Sidsel (Peer reviewed; Journal article, 2019)Efficient production of sugar monomers from lignocellulose is often hampered by serious bottle-necks in biomass hydrolysis. The present study reveals that ultra-sonication assisted pretreatment following autoclaving, termed ... -
Enzyme-Constrained Models and Omics Analysis of Streptomyces coelicolor Reveal Metabolic Changes that Enhance Heterologous Production
Sulheim, Snorre; Kumelj, Tjasa; van Dissel, Dino; Salehzadeh-Yazdi, Ali; Chao, Du; Van Wezel, Gilles P.; Nieselt, Kay; Almaas, Eivind; Wentzel, Alexander; Kerkhoven, Eduard J (Journal article; Peer reviewed, 2020)Many biosynthetic gene clusters (BGCs) require heterologous expression to realize their genetic potential, including silent and metagenomic BGCs. Although the engineered Streptomyces coelicolor M1152 is a widely used host ... -
Experimental and Modeling Studies of Fermentative Butanol Production from Lignocellulosic Sugars
Birgen, Cansu (Doctoral theses at NTNU;2019:378, Doctoral thesis, 2019)The primary focus of the work performed and summarized in this thesis was to understand and improve anaerobic fermentation of lignocellulosic sugars for butanol production by Clostridia. Experimental studies form the basis ... -
Intracellular metabolite pool changes in response to nutrient depletion induced metabolic switching in Streptomyces coelicolor
Wentzel, Alexander; Sletta, Håvard; Wellington, E.M.H.; Ellingsen, Trond Erling; Bruheim, Per (Journal article; Peer reviewed, 2012)Abstract: A metabolite profiling study of the antibiotic producing bacterium Streptomyces coelicolor A3(2) has been performed. The aim of this study was to monitor intracellular metabolite pool changes occurring as strains ... -
Isolation and genome sequencing of four Arctic marine Psychrobacter strains exhibiting multicopper oxidase activity
Shojaei Moghadam, Morteza; Albersmeier, Andreas; Winkler, Anika; Cimmino, Lorenzo; Rise, Kjersti; Hohmann-Marriott, Martin Frank; Kalinowski, Jörn; Rückert, Christian; Wentzel, Alexander; Lale, Rahmi (Journal article; Peer reviewed, 2016)Background Marine cold-temperature environments are an invaluable source of psychrophilic microbial life for new biodiscoveries. An Arctic marine bacterial strain collection was established consisting of 1448 individual ... -
Kinetic Study of Butanol Production from Mixtures of Glucose and Xylose and Investigation of Different Pre-growth Strategies
Birgen, Cansu; Berglihn, Olaf Trygve; Preisig, Heinz A.; Wentzel, Alexander (Journal article; Peer reviewed, 2019)This study proposes a dynamic model that describes key characteristics of fermentative butanol production from glucose and xylose mixtures. The model has 12 parameters and incorporates noncompetitive inhibitory interaction ... -
Micro-scale studies of microbialinduced carbonate precipitation for the application of biocementation
Zehner, Jennifer (Doctoral theses at NTNU;2021:59, Doctoral thesis, 2021)Cement, which is used as a binding material in conventional concrete, is the second most consumed material by humans, after water. The production process of cement, however, causes a high emission of CO2 and contributes ... -
Microbial-induced calcium carbonate precipitation: An experimental toolbox for in situ and real-time investigation of micro-scale pH evolution
Zehner, Jennifer Simone; Røyne, Anja; Wentzel, Alexander; Sikorski, Pawel (Peer reviewed; Journal article, 2020)Concrete is the second most consumed product by humans, after water. However, the production of conventional concrete causes more than 5% of anthropogenic CO2 emissions and therefore there is a need for emission-reduced ... -
NMR-Structural Analysis of Proteins from Oil Reservoir Metagenomes
Erlandsen, Hilde Opdahl (Master thesis, 2015)Microorganisms are found ubiquitously around the world, where they survive as a result of their specialized biochemical reactions and enzymes. In the extreme environment of deep sea oil reservoirs, the microbes have to ... -
Novel archaeal thermostable cellulases from an oil reservoir metagenome
Lewin, Anna; Zhou, Jinglie; Pham, Vu Thuy Trang; Haugen, Tone; El Zeiny, Mohamed; Aarstad, Olav Andreas; Liebl, Wolfgang; Wentzel, Alexander; Liles, Mark R. (Journal article; Peer reviewed, 2017)Microbial assemblages were sampled from an offshore deep sub-surface petroleum reservoir 2.5 km below the ocean floor off the coast of Norway, providing conditions of high temperature and pressure, to identify new thermostable ... -
Optimized submerged batch fermentation strategy for systems scale studies of metabolic switching in Streptomyces coelicolor A3(2)
Wentzel, Alexander; Bruheim, Per; Øverby, Anders; Jakobsen, Øyvind Mejdell; Sletta, Håvard; Omara, Walid A.M.; Hodgson, David A.; Ellingsen, Trond Erling (Journal article; Peer reviewed, 2012)Background: Systems biology approaches to study metabolic switching in Streptomyces coelicolor A3(2) depend on cultivation conditions ensuring high reproducibility and distinct phases of culture growth and secondary ... -
Predicting strain engineering strategies using iKS1317: a genome-scale metabolic model of Streptomyces coelicolor
Kumelj, Tjasa; Sulheim, Snorre; Wentzel, Alexander; Almaas, Eivind (Journal article; Peer reviewed, 2018)Streptomyces coelicolor is a model organism for the Actinobacteria, a phylum known to produce an extensive range of different bioactive compounds that include antibiotics currently used in the clinic. Biosynthetic gene ... -
Psychrobacter spp. from an Arctic marine environment: Versatile candidates for the degradation of phenolic compounds
Shojaei-Moghadam, Morteza (Doctoral thesis at NTNU;2016:133, Doctoral thesis, 2016)Phenol and its derivatives have challenged pollution/waste management due to being structurally recalcitrant to degradation processes. Bio-based processes continue to be the preferred choice for removal of phenols due to ...