Browsing NTNU Open by Author "Steigedal, Magnus"
Now showing items 1-15 of 15
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A novel antimycobacterial compound acts as an intracellular iron chelator
Dragset, Marte Singsås; Poce, Giovanna; Alfonso, Salvatore; Padilla-Benavides, Teresita; Ioerger, Thomas R.; Kaneko, Takushi; Sacchettini, James C.; Biava, Mariangela; Parish, Tanya; Argüello, José M.; Steigedal, Magnus; Rubin, Eric J. (Journal article; Peer reviewed, 2015)Efficient iron acquisition is crucial for the pathogenesis of Mycobacterium tuberculosis. Mycobacterial iron uptake and metabolism are therefore attractive targets for antitubercular drug development. Resistance mutations ... -
Benzoic Acid-Inducible Gene Expression in Mycobacteria
Dragset, Marte Singsås; Barczak, Amy K.; Kannan, Nisha; Mærk, Mali; Flo, Trude Helen; Valla, Svein; Rubin, Eric J.; Steigedal, Magnus (Journal article; Peer reviewed, 2015)Conditional expression is a powerful tool to investigate the role of bacterial genes. Here, we adapt the Pseudomonas putida-derived positively regulated XylS/Pm expression system to control inducible gene expression in ... -
Dynamics of immune effector mechanisms during infection with Mycobacterium aviumin C57BL/6 mice
Haug, Markus; Awuh, Jane Atesoh; Steigedal, Magnus; Kojen, June Frengen; Marstad, Anne; Nordrum, Ivar Skjåk; Halaas, Øyvind; Flo, Trude Helen (Journal article; Peer reviewed, 2013)Opportunistic infections with non-tuberculous mycobacteria such as Mycobacterium avium are receiving renewed attention because of increased incidence and difficulties in treatment. As for other mycobacterial infections, a ... -
Genetic Variation/Evolution and Differential Host Responses Resulting from In-Patient Adaptation of Mycobacterium avium
Kannan, Nisha; Lai, Yi-Pin; Haug, Markus; Lilleness, M.K.; Bakke, Siril Skaret; Marstad, Anne; Hov, Håkon; Naustdal, Thor; Afset, Jan Egil; Ioerger, Thomas R.; Flo, Trude Helen; Steigedal, Magnus (Journal article; Peer reviewed, 2019)Members of the Mycobacterium avium complex (MAC) are characterized as nontuberculosis mycobacteria and are pathogenic mainly in immunocompromised individuals. MAC strains show a wide genetic variability, and there is growing ... -
Genome-wide Phenotypic Profiling Identifies and Categorizes Genes Required for Mycobacterial Low Iron Fitness
Dragset, Marte Singsås; Ioerger, Thomas R.; Zhang, Yanjia; Mærk, Mali; Ginbot, Zekarias; Flo, Trude Helen; Rubin, Eric J; Steigedal, Magnus (Journal article; Peer reviewed, 2019)Iron is vital for nearly all living organisms, but during infection, not readily available to pathogens. Infectious bacteria therefore depend on specialized mechanisms to survive when iron is limited. These mechanisms make ... -
Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence
Dragset, Marte Singsås; Ioerger, Thomas R.; Loevenich, Maja; Haug, Markus; Sivakumar, Niruja; Marstad, Anne; Cardona, Pere-Joan; Klinkenberg, Geir; Rubin, Eric J.; Steigedal, Magnus; Flo, Trude Helen (Journal article; Peer reviewed, 2019)Nontuberculous mycobacterial infections caused by the opportunistic pathogen Mycobacterium avium subsp. hominissuis (MAH) are currently receiving renewed attention due to increased incidence combined with difficult treatment. ... -
How the antimicrobial Protein, Lipocalin 2, affects the Establishment of Microbiota in the Gut of Mice.
Nedberg, Nora Hersoug (Master thesis, 2012)Several studies have suggested that the host genetics may influence the composition of gut microbiota, but few genes involved in host control have been proposed (Sekirov et al., 2010). Lipocalin 2 (Lcn2) prevents growth ... -
Investigation of changes in immunostimulatory properties of clinical Mycobacterium avium strains over time of infection
Dietrich, Markus (Master thesis, 2013) -
Lipocalin 2 imparts selective pressure on bacterial growth in the bladder and is elevated in women with urinary tract infection
Steigedal, Magnus; Marstad, Anne; Haug, Markus; Damås, Jan Kristian; Strong, Roland K.; Roberts, Pacita L.; Himpsl, Stephanie D.; Stapleton, Ann; Hooton, Thomas M.; Mobley, Harry L.T.; Hawn, Thomas R.; Flo, Trude Helen (Journal article; Peer reviewed, 2014)Competition for iron is a critical component of successful bacterial infections, but the underlying in vivo mechanisms are poorly understood. We have previously demonstrated that lipocalin 2 (LCN2) is an innate immunity ... -
Mycobacterial Esx-3 Requires Multiple Components for Iron Acquisition
Siegrist, M. Sloan; Steigedal, Magnus; Ahmad, Rushdy; Mehra, Alka; Dragset, Marte Singsås; Schuster, Brian M.; Phillips, Jennifer A.; Carr, Steven A.; Rubin, Eric J (Journal article; Peer reviewed, 2014)The type VII secretion systems are conserved across mycobacterial species and in many Gram-positive bacteria. While the well-characterized Esx-1 pathway is required for the virulence of pathogenic mycobacteria and conjugation ... -
Mycobacterium smegmatis Vaccine Vector Elicits CD4+ Th17 and CD8+ Tc17 T Cells With Therapeutic Potential to Infections With Mycobacterium avium
Kannan, Nisha; Haug, Markus; Steigedal, Magnus; Flo, Trude Helen (Peer reviewed; Journal article, 2020)Mycobacterium avium (Mav) complex is increasingly reported to cause non-tuberculous infections in individuals with a compromised immune system. Treatment is complicated and no vaccines are available. Previous studies have ... -
Pulsed-field gel electrophoresis profiling and comparison of immunostimulatory properties of clinical Mycobacterium avium Complex isolates
Lilleness, M. Kathleen (Master thesis, 2013) -
The Azotobacter vinelandii mannuronan C5-epimerases: their biological functions and new tools useful for their future in vivo biotechnological application
Steigedal, Magnus (Doktoravhandlinger ved NTNU, 1503-8181; 2006:18, Doctoral thesis, 2006)Alginate, an industrially widely used polysaccharide composed of β-D-mannuronic acid (M) and α-L-guluronic acid (G), is produced by brown algae and certain bacteria (e.g. pseudomonads and Azotobacter vinelandii). The ... -
The Role of Lipocalin 2 in the Shaping of the Gastrointestinal Microbiota and Development of Inflammatory Bowel Disease in Mice
Leistad, Espen Bang (Master thesis, 2013)The gastrointestinal microbiota have long been associated with health and disease in humans. Inflammatory bowel disease (IBD) is a disease thought to arise from a dysfunctional immune response to the commensal microbiota ... -
Understanding and preventing Mycobacterium avium infections
Kannan, Nisha (Doctoral theses at NTNU;2018:178, Doctoral thesis, 2018)Mycobacterium avium (Mav) complex (MAC) is a group of slow growing, environmental opportunist pathogens, that have been associated with animal and human infections. Mav is one of the species belonging to MAC, which can be ...