Browsing NTNU Open by Author "Krossa, Sebastian"
Now showing items 1-10 of 10
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Exploring the viability of measuring zinc and prostate-specific antigen in urine as a diagnostic tool for prostate cancer
Nguyen, Thai Thanh (Master thesis, 2021)Bakgrunn: Nåværende metoder for å diagnostisere prostatakreft benytter seg av inngripende biopsier, som kan føre til komplikasjoner som infeksjon og død. Ikke-inngripende metoder benytter seg av PSA målinger, som ofte er ... -
The genes controlling normal function of citrate and spermine secretion are lost in aggressive prostate cancer and prostate model systems
Rye, Morten Beck; Krossa, Sebastian; Hall, Martina; van Mourik, Casper; Bathen, Tone Frost; Drabløs, Finn; Tessem, May-Britt; Bertilsson, Helena (Peer reviewed; Journal article, 2022)High secretion of the metabolites citrate and spermine is a unique hallmark for normal prostate epithelial cells, and is reduced in aggressive prostate cancer. However, the identity of the genes controlling this biological ... -
KDEL Receptor 1 Contributes to Cell Surface Association of Protein Disulfide Isomerases
Bartels, Anne-Kathrin; Gottert, Sascha; Desel, Christine; Schäfer, Miriam; Krossa, Sebastian; Scheidig, Axel J.; Gotzinger, Joachim; Lorenzen, Inken (Journal article; Peer reviewed, 2019)Background/Aims: Endoplasmic reticulum (ER)-resident proteins with a C-terminal KDEL ER-retention sequence are captured in the Golgi apparatus by KDEL receptors (KDELRs). The binding of such proteins to these receptors ... -
An optimized MALDI MSI protocol for spatial detection of tryptic peptides in fresh frozen prostate tissue
Høiem, Therese Stork; Andersen, Maria Karoline; Martin-Lorenzo, Marta; Longuespée, Rémi; Claes, Britt S.R.; Nordborg, Anna; Dewez, Frédéric; Balluff, Benjamin; Giampà , Marco; Sharma, Animesh; Hagen, Lars; Heeren, Ron M.A.; Bathen, Tone Frost; Giskeødegård, Guro F.; Krossa, Sebastian; Tessem, May-Britt (Peer reviewed; Journal article, 2022)MALDI MS imaging (MSI) is a powerful analytical tool for spatial peptide detection in heterogeneous tissues. Proper sample preparation is crucial to achieve high quality, reproducible measurements. Here we developed an ... -
Redundancy of protein disulfide isomerases in the catalysis of the inactivating disulfide switch in A Disintegrin and Metalloprotease 17
Krossa, Sebastian; Scheidig, Axel J.; Grötzinger, Joachim; Lorenzen, Inken (Journal article; Peer reviewed, 2018)A Disintegrin and Metalloprotease 17 (ADAM17) can cause the fast release of growth factors and inflammatory mediators from the cell surface. Its activity has to be turned on which occurs by various stimuli. The active form ... -
Sample Preparation for Metabolite Detection in Mass Spectrometry Imaging
Andersen, Maria Karoline; Giampà , Marco; Midtbust, Elise; Høiem, Therese Stork; Krossa, Sebastian; Tessem, May-Britt (Peer reviewed; Journal article, 2023) -
A simple preparation protocol for shipping and storage of tissue sections for laser ablation-inductively coupled plasma-mass spectrometry imaging
Buchholz, Rebecca; Krossa, Sebastian; Andersen, Maria Karoline; Holtkamp, Michael; Sperling, Michael; Karst, Uwe; Tessem, May-Britt (Peer reviewed; Journal article, 2022)A rapid and cost-efficient tissue preparation protocol for laser ablation-inductively coupled plasma-mass spectrometry imaging (LA–ICP–MSI) has been developed within this study as an alternative to the current gold standard ... -
Simultaneous Detection of Zinc and Its Pathway Metabolites Using MALDI MS Imaging of Prostate Tissue
Andersen, Maria Karoline; Krossa, Sebastian; Høiem, Therese Stork; Buchholz, Rebecca; Claes, Britt S.R.; Balluff, Benjamin; Ellis, Shane; Richardsen, Elin; Bertilsson, Helena; Heeren, Ron M.A.; Bathen, Tone Frost; Karst, Uwe; Giskeødegård, Guro F.; Tessem, May-Britt (Journal article; Peer reviewed, 2020)Levels of zinc, along with its mechanistically related metabolites citrateand aspartate, are widely reported as reduced in prostate cancer compared to healthytissue and are therefore pointed out as potential cancer biomarkers. ... -
Spatial differentiation of metabolism in prostate cancer tissue by MALDI-TOF MSI
Andersen, Maria Karoline; Høiem, Therese Stork; Claes, Britt S.R.; Balluff, Benjamin; Marta, Martin-Lorenzo; Richardsen, Elin; Krossa, Sebastian; Bertilsson, Helena; Heeren, Ron M.A.; Rye, Morten Beck; Giskeødegård, Guro F.; Bathen, Tone Frost; Tessem, May-Britt (Peer reviewed; Journal article, 2021)Background Prostate cancer tissues are inherently heterogeneous, which presents a challenge for metabolic profiling using traditional bulk analysis methods that produce an averaged profile. The aim of this study was ... -
Visualization of Small Intact Proteins in Breast Cancer FFPE Tissue
Giampà , Marco; Andersen, Maria Karoline; Krossa, Sebastian; Denti, Vanna; Smith, Andrew; Moestue, Siver Andreas (Peer reviewed; Journal article, 2023)