• A novel method for the efficient and selective identification of 5-hydroxymethylcytosine in genomic DNA 

      Robertson, Adam Brian; Dahl, John Arne; Vågbø, Cathrine Broberg; Tripathi, P; Krokan, Hans Einar; Klungland, Arne (Journal article; Peer reviewed, 2011)
      Recently, 5-hydroxymethylcytosine (5hmC) was identified in mammalian genomic DNA. The biological role of this modification remains unclear; however, identifying the genomic location of this modified base will assist in ...
    • AID expression in B-cell lymphomas causes accumulation of genomic uracil and a distinct AID mutational signature 

      Pettersen, Henrik Sahlin; Galashevskaya, Anastasia; Doseth, Berit; Sousa, Mirta; Sarno, Antonio; Visnes, Torkild; Aas, Per Arne; Liabakk, Nina-Beate; Slupphaug, Geir; Sætrom, Pål; Kavli, Bodil Merete; Krokan, Hans Einar (Journal article; Peer reviewed, 2014)
      tThe most common mutations in cancer are C to T transitions, but their origin has remained elusive.Recently, mutational signatures of APOBEC-family cytosine deaminases were identified in many com-mon cancers, suggesting ...
    • ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA 

      Van den Born, Erwin; Vågbø, Cathrine Broberg; Songe-Møller, Lene; Leihne, VIbeke; Lien, Guro Flor; Leszczynska, G; Malkiewicz, A; Krokan, Hans Einar; Kirpekar, F; Klungland, Arne; Falnes, Pål (Journal article; Peer reviewed, 2011)
      Mammals have nine different homologues (ALKBH1–9) of the Escherichia coli DNA repair demethylase AlkB. ALKBH2 is a genuine DNA repair enzyme, but the in vivo function of the other ALKBH proteins has remained elusive. It ...
    • DNA base modifications in honey bee and fruit fly genomes suggest an active demethylation machinery with species- and tissue-specific turnover rates 

      Rasmussen, Erik; Vågbø, Cathrine Broberg; Münch, Daniel; Krokan, Hans Einar; Klungland, Arne; Amdam, Gro Vang; Dahl, John Arne (Journal article; Peer reviewed, 2016)
      Well-known epigenetic DNA modifications in mammals include the addition of a methyl group and a hydroxyl group to cytosine, resulting in 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) respectively. In contrast, ...
    • Excerpts from the 1st international NTNU symposium on current and future clinical biomarkers of cancer: innovation and implementation, June 16th and 17th 2016, Trondheim, Norway 

      Robles, Ana I.; Olsen, Karina Standahl; Tsui, Dana W.T.; Georgoulias, Vassilis; Creaney, Jenette; Dobra, Katalin; Vyberg, Mogens; Minato, Nagahiro; Anders, Robert A.; Børresen-Dale, Anne-Lise; Zhou, Jianwei; Sætrom, Pål; Nielsen, Boye Schnack; Kirschner, Michaela B.; Krokan, Hans Einar; Papadimitrakopoulou, Vassiliki; Tsamardinos, Ioannis; Røe, Oluf Dimitri (Journal article; Peer reviewed, 2016)
      The goal of biomarker research is to identify clinically valid markers. Despite decades of research there has been disappointingly few molecules or techniques that are in use today. The “1st International NTNU Symposium ...
    • Influence of common genetic variation on lung cancer risk: meta-analysis of 14 900 cases and 29 485 controls 

      Timofeeva, M N; Hung, R J; Rafnar, T; Christiani, D C; Field, J K; Bickeboller, H; Risch, A; McKay, J D; Wang, Y; Dai, J; Gaborieau, V; McLaughlin, J; Brenner, D; Narod, S A; Caporaso, NE.; Albanes, D; Thun, M; Eisen, T; Wichmann, H E; Rosenberger, A; Han, Y; Chen, W; Zhu, D; Spitz, M; Wu, X; Pande, M; Zhao, Y; Zaridze, D; Szeszenia-Dabrowska, N; Lissowska, J; Rudnai, P; Fabianova, E; Mates, D; Bencko, V; Foretova, L; Janout, V; Krokan, Hans Einar; Gabrielsen, Maiken Elvestad; Skorpen, Frank; Vatten, Lars Johan; Njølstad, Inger; Chen, C; Goodman, G; Lathrop, M; Benhamou, S; Vooder, T; Valk, K; Nelis, M; Metspalu, Andres; Raji, O; Chen, Y; Gosney, J; Liloglou, T; Muley, T; Dienemann, H; Thorleifsson, G; Shen, H.; Stefansson, Kari; Brennan, Paul; Amos, C I; Houlston, Richard; Landi, M T (Journal article; Peer reviewed, 2012)
    • Protozoan ALKBH8 Oxygenases Display both DNA Repair and tRNA Modification Activities 

      Zdzalik, Daria; Vågbø, Cathrine Broberg; Kirpekar, F; Davydova, Erna; Puścian, Alicja; Maciejewska, Agnieszka M.; Krokan, Hans Einar; Klungland, Arne; Tudek, Barbara; Van den Born, Erwin; Falnes, Pål (Journal article; Peer reviewed, 2014)
      The ALKBH family of Fe(II) and 2-oxoglutarate dependent oxygenases comprises enzymes that display sequence homology to AlkB from E. coli, a DNA repair enzyme that uses an oxidative mechanism to dealkylate methyl and etheno ...
    • Red versus blue light illumination in hexyl 5-aminolevulinate photodynamic therapy: the influence of light color and irradiance on the treatment outcome in vitro 

      Helander, Linda; Krokan, Hans Einar; Johnsson, Anders Carl G.; Gederaas, Odrun Arna; Plaetzer, Kristjan (Journal article; Peer reviewed, 2014)
      Abstract. Hexyl 5-aminolevulinate (HAL) is a lipophilic derivative of 5-aminolevulinate, a key intermediate in biosynthesis of the photosensitizer protoporphyrin IX (PpIX). The photodynamic efficacy and cell death mode ...
    • Roles of Trm9-and ALKBH8-like proteins in the formation of modified wobble uridines in Arabidopsis tRNA 

      Leihne, VIbeke; Kirpekar, F; Vågbø, Cathrine Broberg; Van den Born, Erwin; Krokan, Hans Einar; Grini, Paul Eivind; Meza, Trine Johansen; Falnes, Pål (Journal article; Peer reviewed, 2011)
      Uridine at the wobble position of tRNA is usually modified, and modification is required for accurate and efficient protein translation. In eukaryotes, wobble uridines are modified into 5-methoxycarbonylmethyluridine (mcm ...
    • Small-molecule inhibitor of OGG1 suppresses proinflammatory gene expression and inflammation 

      Visnes, Torkild; Armando, Cazares-Körner; Wenjing, Hao; Wallner, Olov; Masuyer, Geoffrey; Loseva, Olga; Mortusewicz, Oliver; Wiita, Elisee; Sarno, Antonio; Manoilov, Aleksandr; Astorga-Wells, Juan; Jemth, Ann-Sofie; Pan, Lang; Sanjiv, Kumar; Karsten, Stella; Gokturk, Camilla; Grube, Maurice; Evert J, Homan; Hanna, Bishoy Magdi Fekry; Cynthia, Paulin BJ; pham, Therese; Rasti, Azita; Warpman Berglund, Ulrika; von Nikolai, Catharina; Benitez-Buelga, Carlos; Koolmeister, Tobias; Ivanic, Dag; Iliev, Petar; Scobie, Martin; Krokan, Hans Einar; Baranczewski, Pawel; Artursson, Per; Altun, Mikael; Jenmalm-Jensen, Annika; Kalderen, Christina; Xueqing, Ba; Zubarev, Roman A.; Stenmark, Pål; Boldogh, Istvan; Helleday, Thomas (Journal article; Peer reviewed, 2018)
      The onset of inflammation is associated with reactive oxygen species and oxidative damage to macromolecules like 7,8-dihydro-8-oxoguanine (8-oxoG) in DNA. Because 8-oxoguanine DNA glycosylase 1 (OGG1) binds 8-oxoG and ...
    • The causal role of smoking in anxiety and depression: a Mendelian randomization analysis of the HUNT study 

      Bjørngaard, Johan Håkon; Gunnell, David; Gabrielsen, Maiken Elvestad; Davey Smith, George; Skorpen, Frank; Krokan, Hans Einar; Vatten, Lars Johan; Romundstad, Pål Richard (Journal article; Peer reviewed, 2013)
      Cigarette smoking is strongly associated with mental illness but the causal direction of the association is uncertain. We investigated the causal relationship between smoking and symptoms of anxiety and depression in the ...
    • The DNA dioxygenase ALKBH2 protects Arabidopsis thaliana against methylation damage 

      Meza, Trine Johansen; Moen, Marivi; Vågbø, Cathrine Broberg; Krokan, Hans Einar; Klungland, Arne; Grini, Paul Eivind; Falnes, Pål (Journal article; Peer reviewed, 2012)
      The Escherichia coli AlkB protein (EcAlkB) is a DNA repair enzyme which reverses methylation damage such as 1-methyladenine (1-meA) and 3-methylcytosine (3-meC). The mammalian AlkB homologues ALKBH2 and ALKBH3 display ...
    • Tissue responses to hexyl 5-aminolevulinate-induced photodynamic treatment in syngeneic orthotopic rat bladder cancer model: possible pathways of action 

      Arum, Carl-Jørgen; Gederaas, Odrun Arna; Larsen, Eivind La Puebla; Randeberg, Lise Lyngsnes; Hjelde, Astrid; Krokan, Hans Einar; Svaasand, Lars O.; Chen, Duan; Zhao, Chun-Mei (Journal article; Peer reviewed, 2011)
      Orthotopic bladder cancer model in rats mimics human bladder cancer with respect to urothelial tumorigenesis and progression. Utilizing this model at pT1 (superficial stage), we analyze the tissue responses to hexyl ...
    • Transcription profiling during the cell cycle shows that a subset of Polycomb-targeted genes is upregulated during DNA replication 

      Diaz, Javier Pena; Hegre, Siv Anita; Anderssen, Endre; Aas, Per Arne; Mjelle, Robin; Gilfillan, Gregor Duncan; Lyle, Robert; Drabløs, Finn; Krokan, Hans Einar; Sætrom, Pål (Journal article; Peer reviewed, 2013)
    • UNG-initiated base excision repair is the major repair route for 5-fluorouracil in DNA, but 5-fluorouracil cytotoxicity depends mainly on RNA incorporation 

      Pettersen, Henrik Sahlin; Visnes, Torkild; Vågbø, Cathrine Broberg; Svaasand, Eva K; Doseth, Berit; Slupphaug, Geir; Kavli, Bodil Merete; Krokan, Hans Einar (Journal article; Peer reviewed, 2011)
      Cytotoxicity of 5-fluorouracil (FU) and 5-fluoro-2′-deoxyuridine (FdUrd) due to DNA fragmentation during DNA repair has been proposed as an alternative to effects from thymidylate synthase (TS) inhibition or RNA incorporation. ...
    • Uracil Accumulation and Mutagenesis Dominated by Cytosine Deamination in CpG Dinucleotides in Mice Lacking UNG and SMUG 

      Alsøe, Lene; Sarno, Antonio; Carracedo Huroz, Sergio; Domanska, Diana Ewa; Dingler, Felix; Lirussi, Lisa; Sengupta, Tanima; Tekin, Nuriye Basdag; Jobert, Laure; Alexandrov, Ludmil B.; Galashevskaya, Anastasia; Rada, Cristina; Sandve, Geir Kjetil; Rognes, Torbjørn; Krokan, Hans Einar; Nilsen, Hilde Loge (Journal article; Peer reviewed, 2017)
      Both a DNA lesion and an intermediate for antibody maturation, uracil is primarily processed by base excision repair (BER), either initiated by uracil-DNA glycosylase (UNG) or by single-strand selective monofunctional ...
    • Uracil-DNA glycosylase UNG1 isoform variant supports class switch recombination and repairs nuclear genomic uracil 

      Sarno, Antonio; Lundbæk, Marie Benner; Liabakk, Nina-Beate; Aas, Per Arne; Mjelle, Robin; Hagen, Lars; Sousa, Mirta; Krokan, Hans Einar; Kavli, Bodil Merete (Journal article; Peer reviewed, 2019)
      UNG is the major uracil-DNA glycosylase in mammalian cells and is involved in both error-free base excision repair of genomic uracil and mutagenic uracil-processing at the antibody genes. However, the regulation of UNG in ...