Browsing NTNU Open by Author "Flo, Trude Helen"
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Analysis of host immune parameters during M. avium infection
Frengen, June (Master thesis, 2012)The goal of this study was to establish and optimize methods to analyze effector cytokine levels and other parameters in murine tissue and plasma samples. These methods should then be used to study the role of regulatory ... -
Analysis of mycobacteria-specific T cell functions in HIV-infected individuals and healthy controls
Habtamu, Adnew Chonchoro (Master thesis, 2012)Tuberculosis caused by Mycobacterium tuberculosis (MTb) continues to represent a major public health problem worldwide and it is the main cause of death among people living with HIV/AIDS. Mycobacteria-specific CD4 T cells ... -
Analysis of mycobacteria-specific T cell functions in HIV-infected individuals and healthy controls
Chonchoro, Habtamu Adnew (Master thesis, 2012)Tuberculosis caused by Mycobacterium tuberculosis (MTb) continues to represent a major public health problem worldwide and it is the main cause of death among people living with HIV/AIDS. Mycobacteria-specific CD4 T cells ... -
Benzoic Acid-Inducible Gene Expression in Mycobacteria
Dragset, Marte Singsås; Barczak, Amy K.; Kannan, Nisha; Mærk, Mali; Flo, Trude Helen; Valla, Svein; Rubin, Eric J.; Steigedal, Magnus (Journal article; Peer reviewed, 2015)Conditional expression is a powerful tool to investigate the role of bacterial genes. Here, we adapt the Pseudomonas putida-derived positively regulated XylS/Pm expression system to control inducible gene expression in ... -
Characterization of the C-type lectin receptor Mincle in Mycobacterium avium-infected macrophages
Sørbø, Håvard Gullik (Master thesis, 2016)Non-tuberculous diseases caused by opportunistic pathogens such as Mycobacterium avium are becoming more prevalent and has surpassed tuberculosis in developed countries. Trehalose6,6-dimycolate (TDM) is recognized by the ... -
Counting mycobacteria in infected human cells and mouse tissue: a comparison between qPCR and CFU
Pathak, Sharad; Awuh, Jane Atesoh; Leversen, Nils Anders; Flo, Trude Helen; Åsjø, Birgitta (Journal article; Peer reviewed, 2012)Due to the slow growth rate and pathogenicity of mycobacteria, enumeration by traditional reference methods like colony counting is notoriously time-consuming, inconvenient and biohazardous. Thus, novel methods that rapidly ... -
Dynamics of immune effector mechanisms during infection with Mycobacterium aviumin C57BL/6 mice
Haug, Markus; Awuh, Jane Atesoh; Steigedal, Magnus; Kojen, June Frengen; Marstad, Anne; Nordrum, Ivar Skjåk; Halaas, Øyvind; Flo, Trude Helen (Journal article; Peer reviewed, 2013)Opportunistic infections with non-tuberculous mycobacteria such as Mycobacterium avium are receiving renewed attention because of increased incidence and difficulties in treatment. As for other mycobacterial infections, a ... -
Expression of Toll-like receptor-3 is enhanced in active inflammatory bowel disease and mediates the excessive release of lipocalin 2
Østvik, Ann Elisabet; Granlund, Atle Van Beelen; Torp, Sverre Helge; Flatberg, Arnar; Beisvag, Vidar; Waldum, Helge; Flo, Trude Helen; Espevik, Terje; Damås, Jan Kristian; Sandvik, Arne Kristian (Journal article; Peer reviewed, 2013)Anti-microbial peptides might influence the pathogenesis and course of inflammatory bowel disease (IBD). We sought to clarify the role of the anti-microbial glycoprotein lipocalin 2 (LCN2) in the colon by determining its ... -
Fecal neutrophil gelatinase-associated lipocalin as a biomarker for inflammatory bowel disease
Thorsvik, Silje; Damås, Jan Kristian; Granlund, Atle van Beelen; Flo, Trude Helen; Bergh, Kåre; Østvik, Ann Elisabet; Sandvik, Arne Kristian (Journal article; Peer reviewed, 2017)Background and Aim Accurate, noninvasive biomarkers are needed to diagnose and monitor inflammatory bowel disease (IBD). Neutrophil gelatinase-associated lipocalin (NGAL), also known as lipocalin 2, is expressed in inflamed ... -
Genetic Variation/Evolution and Differential Host Responses Resulting from In-Patient Adaptation of Mycobacterium avium
Kannan, Nisha; Lai, Yi-Pin; Haug, Markus; Lilleness, M.K.; Bakke, Siril Skaret; Marstad, Anne; Hov, Håkon; Naustdal, Thor; Afset, Jan Egil; Ioerger, Thomas R.; Flo, Trude Helen; Steigedal, Magnus (Journal article; Peer reviewed, 2019)Members of the Mycobacterium avium complex (MAC) are characterized as nontuberculosis mycobacteria and are pathogenic mainly in immunocompromised individuals. MAC strains show a wide genetic variability, and there is growing ... -
Genome-wide Phenotypic Profiling Identifies and Categorizes Genes Required for Mycobacterial Low Iron Fitness
Dragset, Marte Singsås; Ioerger, Thomas R.; Zhang, Yanjia; Mærk, Mali; Ginbot, Zekarias; Flo, Trude Helen; Rubin, Eric J; Steigedal, Magnus (Journal article; Peer reviewed, 2019)Iron is vital for nearly all living organisms, but during infection, not readily available to pathogens. Infectious bacteria therefore depend on specialized mechanisms to survive when iron is limited. These mechanisms make ... -
Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence
Dragset, Marte Singsås; Ioerger, Thomas R.; Loevenich, Maja; Haug, Markus; Sivakumar, Niruja; Marstad, Anne; Cardona, Pere-Joan; Klinkenberg, Geir; Rubin, Eric J.; Steigedal, Magnus; Flo, Trude Helen (Journal article; Peer reviewed, 2019)Nontuberculous mycobacterial infections caused by the opportunistic pathogen Mycobacterium avium subsp. hominissuis (MAH) are currently receiving renewed attention due to increased incidence combined with difficult treatment. ... -
How the antimicrobial Protein, Lipocalin 2, affects the Establishment of Microbiota in the Gut of Mice.
Nedberg, Nora Hersoug (Master thesis, 2012)Several studies have suggested that the host genetics may influence the composition of gut microbiota, but few genes involved in host control have been proposed (Sekirov et al., 2010). Lipocalin 2 (Lcn2) prevents growth ... -
Lipocalin 2 imparts selective pressure on bacterial growth in the bladder and is elevated in women with urinary tract infection
Steigedal, Magnus; Marstad, Anne; Haug, Markus; Damås, Jan Kristian; Strong, Roland K.; Roberts, Pacita L.; Himpsl, Stephanie D.; Stapleton, Ann; Hooton, Thomas M.; Mobley, Harry L.T.; Hawn, Thomas R.; Flo, Trude Helen (Journal article; Peer reviewed, 2014)Competition for iron is a critical component of successful bacterial infections, but the underlying in vivo mechanisms are poorly understood. We have previously demonstrated that lipocalin 2 (LCN2) is an innate immunity ... -
Molecular basis of mycobacterial survival in macrophages
Awuh, Jane Atesoh; Flo, Trude Helen (Journal article; Peer reviewed, 2017)Macrophages play an essential role in the immune system by ingesting and degrading invading pathogens, initiating an inflammatory response and instructing adaptive immune cells, and resolving inflammation to restore ... -
Molecular tools and model systems for studying inflammasome activation
Kim, Haelin (Master thesis, 2019)Upon cellular stress or invasion by pathogens such as Mycobacterium tuberculosis (Mtb), inflammasomes are activated as an innate immune response, an important host-defense mechanism to resist pathogens. Activation of NLRP3 ... -
Mycobacterium smegmatis Vaccine Vector Elicits CD4+ Th17 and CD8+ Tc17 T Cells With Therapeutic Potential to Infections With Mycobacterium avium
Kannan, Nisha; Haug, Markus; Steigedal, Magnus; Flo, Trude Helen (Peer reviewed; Journal article, 2020)Mycobacterium avium (Mav) complex is increasingly reported to cause non-tuberculous infections in individuals with a compromised immune system. Treatment is complicated and no vaccines are available. Previous studies have ... -
N-3 PUFAs induce inflammatory tolerance by formation of KEAP1-containing SQSTM1/p62-bodies and activation of NFE2L2
Mildenberger, Jennifer; Johansson, Ida; Sergin, Ismail; Kjøbli, Eli; Damås, Jan Kristian; Razani, Babak; Flo, Trude Helen; Bjørkøy, Geir (Journal article; Peer reviewed, 2017)Inflammation is crucial in the defense against infections but must be tightly controlled to limit detrimental hyperactivation. Our diet influences inflammatory processes and omega-3 polyunsaturated fatty acids (n-3 PUFAs) ... -
Persistent mycobacteria evade an antibacterial program mediated by phagolysosomal TLR7/8/MyD88 in human primary macrophages
Gidon, Alexandre; Åsberg, Signe; Louet, Claire; Ryan, Liv; Haug, Markus; Flo, Trude Helen (Journal article; Peer reviewed, 2017)Pathogenic mycobacteria reside in macrophages where they avoid lysosomal targeting and degradation through poorly understood mechanisms proposed to involve arrest of phagosomal maturation at an early endosomal stage. A ... -
Photochemical internalization of peptide antigens provides a novel strategy to realize therapeutic cancer vaccination
Haug, Markus; Brede, Gaute; Håkerud, Monika; Nedberg, Anne Grete; Gederaas, Odrun Arna; Flo, Trude Helen; Edwards, Victoria Tudor; Selbo, Pål Kristian; Høgset, Anders; Halaas, Øyvind (Journal article; Peer reviewed, 2018)Effective priming and activation of tumor-specific CD8+ cytotoxic T lymphocytes (CTLs) is crucial for realizing the potential of therapeutic cancer vaccination. This requires cytosolic antigens that feed into the MHC class ...