Blar i NTNU Open på forfatter "Flo, Trude Helen"
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Analysis of host immune parameters during M. avium infection
Frengen, June (Master thesis, 2012)The goal of this study was to establish and optimize methods to analyze effector cytokine levels and other parameters in murine tissue and plasma samples. These methods should then be used to study the role of regulatory ... -
Analysis of mycobacteria-specific T cell functions in HIV-infected individuals and healthy controls
Chonchoro, Habtamu Adnew (Master thesis, 2012)Tuberculosis caused by Mycobacterium tuberculosis (MTb) continues to represent a major public health problem worldwide and it is the main cause of death among people living with HIV/AIDS. Mycobacteria-specific CD4 T cells ... -
Analysis of mycobacteria-specific T cell functions in HIV-infected individuals and healthy controls
Habtamu, Adnew Chonchoro (Master thesis, 2012)Tuberculosis caused by Mycobacterium tuberculosis (MTb) continues to represent a major public health problem worldwide and it is the main cause of death among people living with HIV/AIDS. Mycobacteria-specific CD4 T cells ... -
Benzoic Acid-Inducible Gene Expression in Mycobacteria
Dragset, Marte Singsås; Barczak, Amy K.; Kannan, Nisha; Mærk, Mali; Flo, Trude Helen; Valla, Svein; Rubin, Eric J.; Steigedal, Magnus (Journal article; Peer reviewed, 2015)Conditional expression is a powerful tool to investigate the role of bacterial genes. Here, we adapt the Pseudomonas putida-derived positively regulated XylS/Pm expression system to control inducible gene expression in ... -
Characterization of the C-type lectin receptor Mincle in Mycobacterium avium-infected macrophages
Sørbø, Håvard Gullik (Master thesis, 2016)Non-tuberculous diseases caused by opportunistic pathogens such as Mycobacterium avium are becoming more prevalent and has surpassed tuberculosis in developed countries. Trehalose6,6-dimycolate (TDM) is recognized by the ... -
Counting mycobacteria in infected human cells and mouse tissue: a comparison between qPCR and CFU
Pathak, Sharad; Awuh, Jane Atesoh; Leversen, Nils Anders; Flo, Trude Helen; Åsjø, Birgitta (Journal article; Peer reviewed, 2012)Due to the slow growth rate and pathogenicity of mycobacteria, enumeration by traditional reference methods like colony counting is notoriously time-consuming, inconvenient and biohazardous. Thus, novel methods that rapidly ... -
The Cyclooxygenase 2 inhibitor etoricoxib as adjunctive therapy in tuberculosis impairs macrophage control of mycobacterial growth
Nore, Kristin Grotle; Louet, Claire; Bugge, Marit; Gidon, Alexandre; Jørgensen, Marthe Jøntvedt; Jenum, Synne; Dyrhol-Riise, Anne Ma; Tonby, Kristian; Flo, Trude Helen (Peer reviewed; Journal article, 2023)Background Current tuberculosis treatment regimens could be improved by adjunct host-directed therapies (HDT) targeting host responses. We investigated the antimycobacterial capacity of macrophages from patients with ... -
Development of a Spectral Library for the Discovery of Altered Genomic Events in Mycobacterium avium Associated With Virulence Using Mass Spectrometry-Based Proteogenomic Analysis
Kotimoole, Chinmaya Narayana; Antil, Neelam; Kasaragod, Sandeep; Behera, Santosh Kumar; Aravind, Anjana; Reiling, Norbert; Flo, Trude Helen; Prasad, Thottethodi S K (Peer reviewed; Journal article, 2023)Mycobacterium avium is one of the prominent disease-causing bacteria in humans. It causes lymphadenitis, chronic and extrapulmonary, and disseminated infections in adults, children, and immunocompromised patients. M. avium ... -
Dynamics of immune effector mechanisms during infection with Mycobacterium aviumin C57BL/6 mice
Haug, Markus; Awuh, Jane Atesoh; Steigedal, Magnus; Kojen, June Frengen; Marstad, Anne; Nordrum, Ivar Skjåk; Halaas, Øyvind; Flo, Trude Helen (Journal article; Peer reviewed, 2013)Opportunistic infections with non-tuberculous mycobacteria such as Mycobacterium avium are receiving renewed attention because of increased incidence and difficulties in treatment. As for other mycobacterial infections, a ... -
Epigenetic control of the intestinal epithelium A role for lysine-specific demethylase 1A during homeostasis and disease
Zwiggelaar, R.T.M. (Doctoral theses at NTNU;2021:151, Doctoral thesis, 2021) -
Expression of Toll-like receptor-3 is enhanced in active inflammatory bowel disease and mediates the excessive release of lipocalin 2
Østvik, Ann Elisabet; Granlund, Atle Van Beelen; Torp, Sverre Helge; Flatberg, Arnar; Beisvag, Vidar; Waldum, Helge; Flo, Trude Helen; Espevik, Terje; Damås, Jan Kristian; Sandvik, Arne Kristian (Journal article; Peer reviewed, 2013)Anti-microbial peptides might influence the pathogenesis and course of inflammatory bowel disease (IBD). We sought to clarify the role of the anti-microbial glycoprotein lipocalin 2 (LCN2) in the colon by determining its ... -
Fecal neutrophil gelatinase-associated lipocalin as a biomarker for inflammatory bowel disease
Thorsvik, Silje; Damås, Jan Kristian; Granlund, Atle van Beelen; Flo, Trude Helen; Bergh, Kåre; Østvik, Ann Elisabet; Sandvik, Arne Kristian (Journal article; Peer reviewed, 2017)Background and Aim Accurate, noninvasive biomarkers are needed to diagnose and monitor inflammatory bowel disease (IBD). Neutrophil gelatinase-associated lipocalin (NGAL), also known as lipocalin 2, is expressed in inflamed ... -
Genetic Variation/Evolution and Differential Host Responses Resulting from In-Patient Adaptation of Mycobacterium avium
Kannan, Nisha; Lai, Yi-Pin; Haug, Markus; Lilleness, M.K.; Bakke, Siril Skaret; Marstad, Anne; Hov, Håkon; Naustdal, Thor; Afset, Jan Egil; Ioerger, Thomas R.; Flo, Trude Helen; Steigedal, Magnus (Journal article; Peer reviewed, 2019)Members of the Mycobacterium avium complex (MAC) are characterized as nontuberculosis mycobacteria and are pathogenic mainly in immunocompromised individuals. MAC strains show a wide genetic variability, and there is growing ... -
Genome-wide Phenotypic Profiling Identifies and Categorizes Genes Required for Mycobacterial Low Iron Fitness
Dragset, Marte Singsås; Ioerger, Thomas R.; Zhang, Yanjia; Mærk, Mali; Ginbot, Zekarias; Flo, Trude Helen; Rubin, Eric J; Steigedal, Magnus (Journal article; Peer reviewed, 2019)Iron is vital for nearly all living organisms, but during infection, not readily available to pathogens. Infectious bacteria therefore depend on specialized mechanisms to survive when iron is limited. These mechanisms make ... -
Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence
Dragset, Marte Singsås; Ioerger, Thomas R.; Loevenich, Maja; Haug, Markus; Sivakumar, Niruja; Marstad, Anne; Cardona, Pere-Joan; Klinkenberg, Geir; Rubin, Eric J.; Steigedal, Magnus; Flo, Trude Helen (Journal article; Peer reviewed, 2019)Nontuberculous mycobacterial infections caused by the opportunistic pathogen Mycobacterium avium subsp. hominissuis (MAH) are currently receiving renewed attention due to increased incidence combined with difficult treatment. ... -
Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death
Borges, Jazlyn P.; Sætra, Ragnhild Sofie Ragnhildstveit; Volchuk, Allen; Bugge, Marit; Devant, Pascal; Sporsheim, Bjørnar; Kilburn, Bridget R.; Evavold, Charles L.; Kagan, Jonathan C.; Goldenberg, Neil M.; Flo, Trude Helen; Steinberg, Benjamin Ethan (Peer reviewed; Journal article, 2022)First recognized more than 30 years ago, glycine protects cells against rupture from diverse types of injury. This robust and widely observed effect has been speculated to target a late downstream process common to multiple ... -
How the antimicrobial Protein, Lipocalin 2, affects the Establishment of Microbiota in the Gut of Mice.
Nedberg, Nora Hersoug (Master thesis, 2012)Several studies have suggested that the host genetics may influence the composition of gut microbiota, but few genes involved in host control have been proposed (Sekirov et al., 2010). Lipocalin 2 (Lcn2) prevents growth ... -
In Vivo Microdialysis in Mice Captures Changes in Alzheimer´s Disease Cerebrospinal Fluid Biomarkers Consistent with Developing Pathology
Bjorkli, Christiana; Louet, Claire; Flo, Trude Helen; Hemler, Mary Elizabeth; Sandvig, Axel; Sandvig, Ioanna (Peer reviewed; Journal article, 2021)Background: Preclinical models of Alzheimer’s disease (AD) can provide valuable insights into the onset and progression of the disease, such as changes in concentrations of amyloid-β (Aβ) and tau in cerebrospinal fluid ... -
Lipocalin 2 imparts selective pressure on bacterial growth in the bladder and is elevated in women with urinary tract infection
Steigedal, Magnus; Marstad, Anne; Haug, Markus; Damås, Jan Kristian; Strong, Roland K.; Roberts, Pacita L.; Himpsl, Stephanie D.; Stapleton, Ann; Hooton, Thomas M.; Mobley, Harry L.T.; Hawn, Thomas R.; Flo, Trude Helen (Journal article; Peer reviewed, 2014)Competition for iron is a critical component of successful bacterial infections, but the underlying in vivo mechanisms are poorly understood. We have previously demonstrated that lipocalin 2 (LCN2) is an innate immunity ... -
Molecular basis of mycobacterial survival in macrophages
Awuh, Jane Atesoh; Flo, Trude Helen (Journal article; Peer reviewed, 2017)Macrophages play an essential role in the immune system by ingesting and degrading invading pathogens, initiating an inflammatory response and instructing adaptive immune cells, and resolving inflammation to restore ...