Blar i NTNU Open på forfatter "Zotchev, Sergey"
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Alginate Biosynthesis Factories in Pseudomonas fluorescens : Localization and Correlation with Alginate Production Level
Maleki, Susan; Almaas, Eivind; Zotchev, Sergey; Valla, Svein; Ertesvåg, Helga (Journal article; Peer reviewed, 2016)Pseudomonas fluorescens is able to produce the medically and industrially important exopolysaccharide alginate. The proteins involved in alginate biosynthesis and secretion form a multiprotein complex spanning the inner ... -
Characterization of Bacterial Electron Transport to Extracellular Electron Acceptors
Lamb, Jacob Joseph (Doctoral thesis at NTNU;2016:186, Doctoral thesis, 2016)Photosynthesis in the cyanobacteria Synechocystis sp. PCC 6803 requires both manganese and a large quantity of iron to maintain its electron transport chain and oxygen evolution; however, in most natural cyanobacterial ... -
Class IV lasso peptides synergistically induce proliferation of cancer cells and sensitize them to doxorubicin
Madland, Eva; Felipe Guerrero-Garzón, Jaime; Zehl, Martin; Singh, Madhurendra; Rezaei, Shiva; Aachmann, Finn Lillelund; Courtade, Gaston; Urban, Ernst; Rückert, Christian; Busche, Tobias; Kalinowski, Jörn; Cao, Yan-Ru; Jiang, Yi; Jiang, Cheng-lin; Selivanova, Galina; Zotchev, Sergey (Peer reviewed; Journal article, 2020)Heterologous expression of a biosynthesis gene cluster from Amycolatopsis sp. resulted in the discovery of two unique class IV lasso peptides, felipeptins A1 and A2. A mixture of felipeptins stimulated proliferation of ... -
Genome Mining of actinomycete bacteria for new natural products
Schneider, Olha (Doctoral theses at NTNU;2018:348, Doctoral thesis, 2018)Here, the genome data of seven different strains of Streptomyces and a strain from the Actinoalloteichus genus were analysed for the presence of biosynthetic gene clusters (BGC) encoding for potential new natural products ... -
Genomics of Sponge-Associated Streptomyces spp. Closely Related to Streptomyces albus J1074: Insights into Marine Adaptation and Secondary Metabolite Biosynthesis Potential
Ian, Elena; Malko, Dmitry B.; Sekurova, Olga; Bredholt, Harald; Rückert, Christian; Borisova, Marina E.; Albersmeier, Andreas; Kalinowski, Jörn; Gelfand, Mikhail S.; Zotchev, Sergey (Journal article; Peer reviewed, 2014)A total of 74 actinomycete isolates were cultivated from two marine sponges, Geodia barretti and Phakellia ventilabrum collected at the same spot at the bottom of the Trondheim fjord (Norway). Phylogenetic analyses of ... -
Identification of a New Phosphatase Enzyme Potentially Involved in the Sugar Phosphate Stress Response in Pseudomonas fluorescens
Maleki, Susan; Hrudikova, Radka; Zotchev, Sergey; Ertesvåg, Helga (Journal article; Peer reviewed, 2017)The alginate-producing bacterium Pseudomonas fluorescens utilizes the Entner-Doudoroff (ED) and pentose phosphate (PP) pathways to metabolize fructose, since the upper part of its Embden-Meyerhof-Parnas pathway is defective. ... -
Mining, Analysis and Targeted Activation of Secondary Metabolite Gene Clusters in Streptomyces bambergiensis
Joelsson, Jon Petur (Master thesis, 2015)The discovery of new antibiotics is one of the most urgent tasks facing scientists today. The escalating rate of resistant microbes creates a constant need for new or improved drugs. With the recent progress in genome ... -
New natural products identified by combined genomics-metabolomics profiling of marine Streptomyces sp. MP131-18
Paulus, Constanze; Rebets, Yuriy; Tokovenko, Bogdan; Nadmid, Suvd; Terekhova, Larisa P.; Myronovskyi, Maksym; Zotchev, Sergey; Rückert, Christian; Braig, Simone; Zahler, Stefan; Kalinowski, Jörn; Luzhetskyy, Andriy (Journal article; Peer reviewed, 2017)Marine actinobacteria are drawing more and more attention as a promising source of new natural products. Here we report isolation, genome sequencing and metabolic profiling of new strain Streptomyces sp. MP131-18 isolated ... -
Re-wiring of antibiotic biosynthesis regulation in Streptomyces venezuelae for inducible heterologous production of secondary metabolites
Pawlikowska, Joanna (Master thesis, 2014)Streptomyces venezuelae ATCC10712 which is an object of research investigation during this master thesis belongs to Actinomycetales which are widely recognized as a producer of antibiotics. Secondary metabolites synthesis ... -
Studies Of Secondary Metabolites In Two Marin Bacteria Cultivated Under Different Conditions
Valde, Marte (Master thesis, 2011)This work has focused on the identification of secondary metabolites possessing antibacterial activity in two isolates collected from the Trondheim fjord. The isolate MP 113-05 was collected from a sponge at 60 meters ... -
Synthetic biology for drug discovery: construction of biosensors for detection of endogenously produced bacterial secondary metabolites
Sandbakken, Ingrid Schafroth (Master thesis, 2013)Streptomycete bacteria are a great source of natural bioactive secondary metabolites, some of which are being used as antibiotics and anticancer drugs. Many streptomycetes have the genomic potential for producing 20-30 ... -
Synthetic Biology of Antibiotic Production: Assembly and Re-factoring of Secondary Metabolite Biosynthesis Gene Clusters for Heterologous Expression in Genetically Engineered Bacterial Host
Zhang, Jianhai (Master thesis, 2014)The issues in new antibiotic discovery are pressing, because the frequent re-discovery of antibiotic scaffolds leads to few novel antibiotics discovered, besides, with the widespread use of antibacterial agents, multi-resistant ...