Browsing NTNU Open by Author "Naas, Adrian Ertsås"

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Non-Invasive Analysis of Recombinant mRNA Stability in Escherichia coli by a Combination of Transcriptional Inducer Wash-Out and qRT-PCR

Kucharova, Veronika; Aakvik, Trine; Almaas, Eivind; Naas, Adrian Ertsås; Brautaset, Trygve; Valla, Svein (Journal article; Peer reviewed, 2013)

mRNA stability is one among many parameters that can potentially affect the level of recombinant gene expression in bacteria. Blocking of the entire prokaryotic transcription machinery by addition of rifampicin is commonly ...
Optimzing the 5'-end of Coding Sequences in Recombinant mRNA to achieve high-level Expression in the Bacterium Escherichia coli

Naas, Adrian Ertsås (Master thesis, 2012)

Recombinant protein production in Escherichia coli provides a cheap and efficient way of producing medically and industrially relevant proteins. Sequence features of individual genes and especially their 5 terminal coding ...