Blar i NTNU Open på forfatter "Malmo, Jostein"
-
Advancements in Construction of XylS/Pm Expression Vectors in Gram-positive Bacteria - Evaluated in Bacillus subtilis and Lactobacillus plantarum
Istre, Mette Stausland (Master thesis, 2018)Escherichia coli (E. coli) is the most commonly used host for expression of recombinant proteins. A large fraction of recombinant proteins produced in E. coli are stored in an insoluble form, making ... -
Cell-Type-Specific Transcription of Innate Immune Regulators in response to HMPV Infection
Loevenich, Simon; Malmo, Jostein; Liberg, Ann Magritt; Sherstova, Tatyana; Li, Youxian; Rian, Kristin; Johnsen, Ingvild Bjellmo; Anthonsen, Marit Walbye (Journal article; Peer reviewed, 2019)Human metapneumovirus (HMPV) may cause severe respiratory disease. The early innate immune response to viruses like HMPV is characterized by induction of antiviral interferons (IFNs) and proinflammatory immune mediators ... -
Central carbon metabolite profiling reveals vector-associated differences in the recombinant protein production host Escherichia coli BL21
Garcia Calvo, Laura; Rane, Divyata Vilas; Everson, Nikalet; Humlebrekk, Sigurd Tømmerberg; Mathiassen, Lise Femanger; Mæhlum, Astfrid Helene Morka; Malmo, Jostein; Bruheim, Per (Peer reviewed; Journal article, 2023)The Gram-negative bacterium Escherichia coli is the most widely used host for recombinant protein production, both as an industrial expression platform and as a model system at laboratory scale. The recombinant protein ... -
Chitosan-based nanocarriers for gene- and siRNA-delivery
Malmo, Jostein (Doktoravhandlinger ved NTNU, 1503-8181; 2012:223, Doctoral thesis, 2012)Gene therapy is promising for the treatment of many currently incurable diseases by delivering or silencing specific genes with pDNA or siRNA, respectively. Clinical trials have mostly been conducted with viral or relatively ... -
Cytokine profiles in human metapneumovirus infected children: Identification of genes involved in the antiviral response and pathogenesis
Malmo, Jostein; Moe, Nina; Krokstad, Sidsel; Ryan, Liv; Loevenich, Simon; Johnsen, Ingvild Bjellmo; Espevik, Terje; Nordbø, Svein Arne; Døllner, Henrik; Anthonsen, Marit Walbye (Journal article; Peer reviewed, 2016)Human metapneumovirus (hMPV) causes severe airway infection in children that may be caused by an unfavorable immune response. The nature of the innate immune response to hMPV in naturally occurring infections in children ... -
Effects of Sec-Pathway Modifications on Periplasmatic Translocation of Recombinant Proteins in Escherichia coli
Elvheim, Andrea Iselin (Master thesis, 2018)Recombinant proteins are expressed for use in both research, industry and medicine. The Gram-negative bacteria Escherichia coli (E. coli) is often used as an expression host. It has several properties making it a suitable ... -
Establishing new XylS/Pm based Gram-Negative Protein Production Strains - Evaluating Pseudoalteromonas haloplanktis and Pseudomonas putida
Strypet, Goro Malene Brennmoen (Master thesis, 2018)To date, Escherichia coli is the most used bacterial host for recombinant protein production. Some proteins are, however, hard to express in this species due to a reducing intracellular environment and inclusion body ... -
Improving expression from the XylS / Pm promoter in Corynebacterium glutamicum
Krakeli, Anne Sofie (Master thesis, 2019)Ved hjelp av rekombinant DNA-teknologi kan gener overføres mellom arter og uttrykkes i andre arter enn opphavsarten. Rekombinante proteinr brukes til en rekke formål i ulike industrier, fra mat- og tekstilindustri til ... -
Innate immune responses to human metapneumovirus - Exploring cellular models and molecular mechanisms of interferon regulation and action
Loevenich, Simon (Doctoral theses at NTNU;2020:101, Doctoral thesis, 2020)Luftveisinfeksjoner forårsaket av virus og bakterier er et betydelig folkehelseproblem. Særlig for barn, gamle mennesker og personer med lungesykdom eller svekket immunsystem kan disse infeksjonene være livstruende. For ... -
Nanoparticle Mediated P-Glycoprotein Silencing for Improved Drug Delivery across the Blood-Brain Barrier: A siRNA-Chitosan Approach
Malmo, Jostein; Sandvig, Axel; Vårum, Kjell Morten; Strand, Sabina P. (Journal article; Peer reviewed, 2013)Background: Neonatal intermittent hyperoxia-hypoxia (IHH) is involved in the pathogenesis of retinopathy of prematurity. Whether similar oxygen fluctuations will create pathological changes in the grey and white matter ... -
Translational regulation of periplasmic folding assistants and proteases as a valuable strategy to improve production of translocated recombinant proteins in Escherichia coli
Gawin, Agnieszka Maria; Ertesvåg, Helga; Hartvigsen Hansen, Sine Alise; Malmo, Jostein; Brautaset, Trygve (Peer reviewed; Journal article, 2020)Background Advantages of translocation of recombinant proteins to the periplasm in Escherichia coli include simplified downstream processing, and improved folding and in vivo activity of the target protein. There are, ...