Blar i NTNU Open på forfatter "Jang, Mi"
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Establishment of an Alginate-Based 3D Culture System of C2C12 Cells in Serum Free Medium and Investigation of the Metabolic Profiles
Scheffold, Jana (Master thesis, 2022)In vitro dyrket kjøtt, kjent som syntetisk kjøtt, er et fremvoksende felt innen bioteknologi med bakgrunn i miljøpåvirkning fra tradisjonell kjøttproduksjon og dyrevelferd. Derimot krever storskala produksjon av syntetisk ... -
ETNK1 mutations induce a mutator phenotype that can be reverted with phosphoethanolamine
Fontana, Diletta; Mauri, Mario; Renso, Rossella; Docci, Mattia; Crespiatico, Ilaria; Røst, Lisa Marie; Jang, Mi; Niro, Antonio; D’Aliberti, Deborah; Massimino, Luca; Bertagna, Mayla; Zambrotta, Giovanni; Bossi, Mario; Citterio, Stefania; Crescenzi, Barbara; Fanelli, Francesca; Cassina, Valeria; Corti, Roberta; Salerno, Domenico; Nardo, Luca; Chinello, Clizia; Mantegazza, Francesco; Mecucci, Cristina; Magni, Fulvio; Cavaletti, Guido; Bruheim, Per; Larsen, Steen; Rea, Delphine; Gambacorti-Passerini, Carlo; Piazza, Rocco (Peer reviewed; Journal article, 2020)Recurrent somatic mutations in ETNK1 (Ethanolamine-Kinase-1) were identified in several myeloid malignancies and are responsible for a reduced enzymatic activity. Here, we demonstrate in primary leukemic cells and in cell ... -
Exploring the Effects of Adaptation to Serum-Free Cultivation for Adherent and Suspended HEK293 Cells
Pete, Ellen Sofie (Master thesis, 2021)Cellekultur er en samlebetegnelse på dyrking av celler utenfor organismen de opprinner fra. Dette er et felt med stor vitenskapelig og industriell relevans, blant annet for produksjon av biofarmasøytika og vaksiner, og for ... -
Exploring the metabolic adaptations in CHO-K1 cells cultured in serum free media and the effect of adaptation method utilized
Renjo, Marietta Mellemløkken (Master thesis, 2021)Serum er en flytende forbindelse avledet fra blod. Vanligvis hentes det fra ubedøvede fostre av storfe, og brukes som et supplement til basale vekstmedier. Det er en udefinert blanding med animalsk opprinnelse, noe som ... -
The impact of serum-free culture on HEK293 cells: From the establishment of suspension and adherent serum-free adaptation cultures to the investigation of growth and metabolic profiles
Jang, Mi; Pete, Ellen Sofie; Bruheim, Per (Peer reviewed; Journal article, 2022)Serum-free cultures are preferred for application in clinical cell therapy and facilitate the purification processes of bioproducts, such as vaccines and recombinant proteins. It can replace traditional cell culture - ... -
Isolation and cultivation of primary muscle cells from Lobster (Homarus gammarus)
Jang, Mi; Scheffold, Jana; Bruheim, Per (Peer reviewed; Journal article, 2022) -
Serum-free alginate-C2C12 cells microcapsule culture as an alternative source of animal protein
Scheffold, Jana; Bruheim, Per; Kjesbu, Joachim Sebastian; Jang, Mi (Peer reviewed; Journal article, 2023)Due to the climate change crisis, and environmental impacts of the traditional meat sector, the production of artificial animal protein based on in vitro cell culture technology is proposed as an alternative. Furthermore, ... -
Serum-free cultures of C2C12 cells show different muscle phenotypes which can be estimated by metabolic profiling
Jang, Mi; Scheffold, Jana; Røst, Lisa M.; Cheon, Hyejeong; Bruheim, Per (Peer reviewed; Journal article, 2022)In vitro skeletal muscle cell production is emerging in the field of artificial lab-grown meat as alternative future food. Currently, there is an urgent paradigm shift towards a serum replacement culture system. Surprisingly, ...