Browsing NTNU Open by Author "Gawin, Agnieszka"
Now showing items 1-5 of 5
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Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production
Gawin, Agnieszka; Peebo, Karl; Sebastian, Hans; Ertesvåg, Helga; Irla, Marta Katarzyna; Neubauer, Peter; Brautaset, Trygve (Journal article; Peer reviewed, 2019)Background Bacteria are widely used as hosts for recombinant protein production due to their rapid growth, simple media requirement and ability to produce high yields of correctly folded proteins. Overproduction of ... -
Effects of Sec-Pathway Modifications on Periplasmatic Translocation of Recombinant Proteins in Escherichia coli
Elvheim, Andrea Iselin (Master thesis, 2018)Recombinant proteins are expressed for use in both research, industry and medicine. The Gram-negative bacteria Escherichia coli (E. coli) is often used as an expression host. It has several properties making it a suitable ... -
Engineering the genome of Escherichia coli BL21 for improved secretory production of recombinant proteins
Hansen, Sine Alise Hartvigsen (Master thesis, 2019)Et økt fokus på rekombinante legemidler er sett i den farmasøytiske industrien. De fleste rekombinante legemidler stammer fra eukaryoter, og krever posttranslasjons-modifikasjoner slik som dannelse av disulfid-bindinger. ... -
Study and Engineering of Gram- Negative Bacteria as Hosts for Production of Recombinant Proteins and Biopolymers
Gawin, Agnieszka (Doctoral theses at NTNU;2020:173, Doctoral thesis, 2020)Gram-negative bacteria are a large group of prokaryotic organisms that thrive in diverse environments and differ in terms of structure and metabolism. They are capable of producing a variety of natural compounds and ... -
The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering.
Gawin, Agnieszka; Valla, Svein; Brautaset, Trygve (Journal article; Peer reviewed, 2017)The XylS/Pm regulator/promoter system originating from the Pseudomonas putida TOL plasmid pWW0 is widely used for regulated low- and high-level recombinant expression of genes and gene clusters in Escherichia coli and other ...