Browsing NTNU Open by Author "Gaardløs, Margrethe"
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Computational modeling of the molecular basis for the calcium-dependence of the mannuronan C-5 epimerase AvAlgE6 from Azotobacter vinelandii
Gaardløs, Margrethe; Lervik, Anders; Samsonov, Sergey A. (Peer reviewed; Journal article, 2023)The mannuronan C-5 epimerases catalyze epimerization of β-d-mannuronic acid to α-l-guluronic acid in alginate polymers. The seven extracellular Azotobacter vinelandii epimerases (AvAlgE1–7) are calcium-dependent, and calcium ... -
Enzyme kinetics of mannuronan C-5 epimerase AlgE4 studied by 13C-NMR and CD spectroscopy
Børve, Marta (Master thesis, 2019)Alginater er en gruppe polysakkarider med et bredt anvendelsesområde. De har ulike fysiske egenskaper, som hovedsakelig bestemmes av sekvensensen til de to monomerne, alfa-D-mannuronat (M) og beta-L-guluronat (G). Sekvensen ... -
Identification of a Pivotal Residue for Determining the Block Structure-Forming Properties of Alginate C-5 Epimerases
Stanisci, Annalucia; Tøndervik, Anne; Gaardløs, Margrethe; Lervik, Anders; Skjåk-Bræk, Gudmund; Sletta, Håvard; Aachmann, Finn Lillelund (Peer reviewed; Journal article, 2020)Alginate is a linear copolymer composed of 1→4 linked β-d-mannuronic acid (M) and its epimer α-l-guluronic acid (G). The polysaccharide is first produced as homopolymeric mannuronan and subsequently, at the polymer level, ... -
Mannuronate C-5 epimerases and their use in alginate modification
Beenfeldt Petersen, Agnes; Tøndervik, Anne; Gaardløs, Margrethe; Ertesvåg, Helga; Sletta, Håvard; Aachmann, Finn Lillelund (Journal article; Peer reviewed, 2023)Alginate is a polysaccharide consisting of β-D-mannuronate (M) and α-L-guluronate (G) produced by brown algae and some bacterial species. Alginate has a wide range of industrial and pharmaceutical applications, owing mainly ... -
Mode of action and catalytic mechanisms of alginatemodifying enzymes Insights from the mannuronan C-5 epimerases and a bifunctional epimerase/lyase from Azotobacter vinelandii
Gaardløs, Margrethe (Doctoral theses at NTNU;2021:200, Doctoral thesis, 2021)Enzymes are biological catalysts that work efficiently and specifically to make life possible. Among their countless tasks in living organisms, they are responsible for the diversity of material properties found in nature. ... -
Mutational study of the bifunctional C5-epimerase and alginate lyase AlgE7 from Azotobacter vinlandii
Kongsrud, Karoline (Master thesis, 2020)Alginater er en familie av lineære polysakkarider som består av 1 → 4 bundet β-D-mannuronsyre (M) og α-L-guluronsyre (G). De to sukkermolekylene er C5-epimerer, noe som betyr at de har ulik stereokjemiske konfigurasjonen ... -
Purification and characterization of design mannuronan C5-epimerases
Maya Hjørnevik (Master thesis, 2019)Alginates are a family of linear polysaccharides composed of 1,4-linked β-D-mannuronic acid (M) and α-L-guluronic acid (G) monomer units. The two sugar molecules can only be distinguished by an opposite stereochemical ...