Blar i NTNU Open på forfatter "Winge, Per"
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Simultaneous knockout of multiple LHCF genes using single sgRNAs and engineering of a high-fidelity Cas9 for precise genome editing in marine algae
Sharma, Amit Kumar; Nymark, Marianne; Flo, Snorre; Sparstad, Torfinn; Bones, Atle M.; Winge, Per (Peer reviewed; Journal article, 2021)The CRISPR/Cas9 system is an RNA-guided sequence-specific genome editing tool, which has been adopted for single or multiple gene editing in a wide range of organisms. When working with gene families with functional ... -
Studies of light responses and the development of a transformation system for the benthic diatom Seminavis robusta
Kirupamurthy, Darshan (Master thesis, 2014)Diatoms are a divergent group of organisms with a complex evolutionary background, and belong to the Heterokonta group phylogenetically. Diatoms have gone through several endosymbiotic processes with both green algae and ... -
System responses to equal doses of photosynthetically usable radiation of blue, green, and red light in the marine diatom Phaeodactylum tricornutum
Valle, Kristin Collier; Nymark, Marianne; Aamot, Inga; Hancke, Kasper; Winge, Per; Andresen, Kjersti; Johnsen, Geir; Brembu, Tore; Bones, Atle M. (Journal article; Peer reviewed, 2014)Due to the selective attenuation of solar light and the absorption properties of seawater and seawater constituents, free-floating photosynthetic organisms have to cope with rapid and unpredictable changes in both intensity ... -
Targeted mutagenesis of Δ5 and Δ6 fatty acyl desaturases induce dysregulation of lipid metabolism in Atlantic salmon (Salmo salar)
Jin, Yang; Datsomor, Alex Kojo; Olsen, Rolf Erik; Vik, Jon Olav; Torgersen, Jacob Seilø; Edvardsen, Rolf Brudvik; Wargelius, Anna; Winge, Per; Grammes, Fabian (Peer reviewed; Journal article, 2020)Background With declining wild fish populations, farmed salmon has gained popularity as a source for healthy long-chain highly unsaturated fatty acids (LC-HUFA). However, the introduction of plant oil in farmed salmon ... -
Testing the importance of jasmonate signalling in induction of plant defences upon cabbage aphid (Brevicoryne brassicae) attack
Kusnierczyk, Anna; Tran, Thi Diem Hong; Winge, Per; Jørstad, Tommy Stokmo; Reese, John C; Troczynska, Joanna; Bones, Atle M. (Journal article; Peer reviewed, 2011)Background Phloem-feeding aphids deprive plants of assimilates, but mostly manage to avoid causing the mechanical tissue damage inflicted by chewing insects. Nevertheless, jasmonate signalling that is induced by infestation ... -
The evolution of small GTP binding proteins in cellular organisms. Studies of RAS GTPases in arabidopsis thaliana and the Ral GTPase from Drosophila melanogaster
Winge, Per (Doctoral thesis, 2002)Small GTP binding proteins function as molecular switches which cycles between GTP-bound ON and GDP-bound OFF states, and regulate a wide variety of cellular processes as biological timers. The first characterized member ... -
The IDA-LIKE peptides IDL6 and IDL7 are negative modulators of stress responses in Arabidopsis thaliana
Vie, Ane Kjersti; Najafi, Javad; Winge, Per; Cattan, Ester; Wrzaczek, Michael; Kangasjärvi, Jakko; Miller, Gad; Brembu, Tore; Bones, Atle M. (Journal article; Peer reviewed, 2017)Small signalling peptides have emerged as important cell to cell messengers in plant development and stress responses. However, only a few of the predicted peptides have been functionally characterized. Here, we present ... -
The IDA/IDA-LIKE and PIP/PIP-LIKE gene families in Arabidopsis: phylogenetic relationship, expression patterns and transcriptional effect of the PIPL3 peptide
Vie, Ane Kjersti; Najafi, Javad; Liu, Bin; Winge, Per; Butenko, Melinka Alonso; Hornslien, K; Kumpf, Robert; Aalen, Reidunn B.; Bones, Atle M.; Brembu, Tore (Journal article; Peer reviewed, 2015)Peptide ligands play crucial roles in the life cycle of plants by modulating the innate immunity against pathogens and regulating growth and developmental processes. One well-studied example is INFLORESCENCE DEFICIENT IN ... -
The molecular basis of long chain polyunsaturated fatty acid (LC-PUFA) biosynthesis in Atlantic salmon (Salmo salar L): In vivo functions, functional redundancy and transcriptional regulation of LC-PUFA biosynthetic enzymes
Datsomor, Alex Kojo (Doctoral theses at NTNU;2019:383, Doctoral thesis, 2019)Farmed Atlantic salmon (Salmo salar L.) is a rich source of the omega-3 fatty acids (ω-3 FA), eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, which are linked to health benefits in humans including prevention of ... -
The Myb like transcription factor Phosphorus Starvation Response (PtPSR) controls conditional P acquisition and remodeling in marine microalgae
Sharma, Amit Kumar; Mühlroth, Alice; Jouhet, Juliette; Maréchal, Eric; Alipanah, Leila; Kissen, Ralph; Brembu, Tore; Bones, Atle M.; Winge, Per (Journal article; Peer reviewed, 2019)Phosphorus (P) is one of the limiting macronutrients for algal growth in marine environments. Microalgae have developed adaption mechanisms to P limitation that involve remodelling of internal phosphate resources and ... -
Transgene-free genome editing in marine algae by bacterial conjugation - comparison with biolistic CRISPR/Cas9 transformation
Sharma, Amit Kumar; Nymark, Marianne; Sparstad, Torfinn; Bones, Atle M.; Winge, Per (Journal article; Peer reviewed, 2018)The CRISPR/Cas9 technology has opened the possibility for targeted genome editing in various organisms including diatom model organisms. One standard method for delivery of vectors to diatom cells is by biolistic particle ... -
Whole-cell response to nitrogen depletion in the diatom Phaeodactylum tricornutum
Alipanah, Leila; Rohloff, Jens; Winge, Per; Bones, Atle M.; Brembu, Tore (Journal article; Peer reviewed, 2015)Algal growth is strongly affected by nitrogen (N) availability. Diatoms, an ecologically important group of unicellular algae, have evolved several acclimation mechanisms to cope with N deprivation. In this study, we ...