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dc.contributor.authorWang, Damao
dc.contributor.authorAarstad, Olav Andreas
dc.contributor.authorLi, Jing
dc.contributor.authorMcKee, Lauren S.
dc.contributor.authorSætrom, Gerd Inger
dc.contributor.authorVyas, Anisha
dc.contributor.authorSrivastava, Vaibhav
dc.contributor.authorAachmann, Finn Lillelund
dc.contributor.authorBulone, Vincent
dc.contributor.authorHsieh, Yves S. Y.
dc.date.accessioned2022-03-11T07:36:42Z
dc.date.available2022-03-11T07:36:42Z
dc.date.created2018-10-23T20:16:20Z
dc.date.issued2018
dc.identifier.citationJournal of Agricultural and Food Chemistry. 2018, 66 1435-1443.en_US
dc.identifier.issn0021-8561
dc.identifier.urihttps://hdl.handle.net/11250/2984423
dc.description.abstractMarine multicellular algae are considered promising crops for the production of sustainable biofuels and commodity chemicals. However, their commercial exploitation is currently limited by a lack of appropriate and efficient enzymes for converting alginate into metabolizable building blocks, such as 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH). Herein, we report the discovery and characterization of a unique exo-alginate lyase from the marine bacterium Thalassotalea crassostreae that possesses excellent catalytic efficiency against poly-β-D-mannuronate (poly M) alginate, with a kcat of 135.8 s–1, and a 5-fold lower kcat of 25 s–1 against poly-α-L-guluronate (poly G alginate). We propose that this preference for poly M is due to a structural feature of the protein’s active site. The mode of action and specificity of this enzyme has made it possible to design an effective and environmentally friendly process for the production of DEH and low molecular weight guluronate-enriched alginate.en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.titlePreparation of 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) and guluronic acid-rich alginate using a unique exo-alginate lyase from Thalassotalea crassostreaeen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionacceptedVersionen_US
dc.source.pagenumber1435-1443en_US
dc.source.volume66en_US
dc.source.journalJournal of Agricultural and Food Chemistryen_US
dc.identifier.doi10.1021/acs.jafc.7b05751
dc.identifier.cristin1622888
dc.relation.projectNorges forskningsråd: 226244en_US
cristin.unitcode194,66,15,0
cristin.unitnameInstitutt for bioteknologi og matvitenskap
cristin.ispublishedtrue
cristin.fulltextpreprint
cristin.qualitycode2


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