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dc.contributor.authorFromm, Bastian
dc.contributor.authorTarbier, Marcel
dc.contributor.authorSmith, Oliver
dc.contributor.authorMármol-Sánchez, Emilio
dc.contributor.authorDalén, Love
dc.contributor.authorGilbert, Marcus Thomas Pius
dc.contributor.authorFriedländer, Marc R.
dc.identifier.citationRNA: A publication of the RNA Society. 2021, 27 324-334.en_US
dc.description.abstractDNA sequencing is the current key technology for historic or ancient biological samples and has led to many exciting discoveries in the field of paleogenomics. However, functional insights into tissue identity, cellular composition, or gene regulation cannot be gained from DNA. Recent analyses have shown that, under favorable conditions, RNA can also be sequenced from ancient samples, enabling studies at the transcriptomic and regulatory level. Analyzing ancient RNA data from a Pleistocene canid, we find hundreds of intact microRNAs that are taxonomically informative, show tissue specificity and have functionally predictive characteristics. With an extraordinary age of 14,300 yr, these microRNA sequences are by far the oldest ever reported. The authenticity of the sequences is further supported by (i) the presence of canid/Caniformia-specific sequences that never evolved outside of this clade, (ii) tissue-specific expression patterns (cartilage, liver, and muscle) that resemble those of modern dogs, and (iii) RNA damage patterns that are clearly distinct from those of fresh samples. By performing computational microRNA-target enrichment analyses on the ancient sequences, we predict microRNA functions consistent with their tissue pattern of expression. For instance, we find a liver-specific microRNA that regulates carbohydrate metabolism and starvation responses in canids. In summary, we show that straightforward paleotranscriptomic microRNA analyses can give functional glimpses into tissue identity, cellular composition, and gene regulatory activity of ancient samples and biological processes that took place in the Pleistocene, thus holding great promise for deeper insights into gene regulation in extinct animals based on ancient RNA sequencing.en_US
dc.publisherCold Spring Harbor Laboratory Press for the RNA Societyen_US
dc.rightsNavngivelse-Ikkekommersiell 4.0 Internasjonal*
dc.titleAncient microRNA profiles of 14,300-yr-old canid samples confirm taxonomic origin and provide glimpses into tissue-specific gene regulation from the Pleistoceneen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.source.journalRNA: A publication of the RNA Societyen_US

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Navngivelse-Ikkekommersiell 4.0 Internasjonal
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