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dc.contributor.authorPandey, Radha Raman
dc.contributor.authorDelfino, Elena
dc.contributor.authorHomolka, David
dc.contributor.authorRoithova, Adriana
dc.contributor.authorChen, Kuan-Ming
dc.contributor.authorLi, Lingyun
dc.contributor.authorFranco, Giulia
dc.contributor.authorVågbø, Cathrine Broberg
dc.contributor.authorTaillebourg, Emmanuel
dc.contributor.authorFauvarque, Marie-Odile
dc.contributor.authorPillai, Ramesh S.
dc.date.accessioned2021-05-10T09:53:29Z
dc.date.available2021-05-10T09:53:29Z
dc.date.created2021-01-24T17:22:13Z
dc.date.issued2020
dc.identifier.citationCell reports. 2020, 32 (7), .en_US
dc.identifier.issn2211-1247
dc.identifier.urihttps://hdl.handle.net/11250/2754590
dc.description.abstractThe 5′ end of eukaryotic mRNAs is protected by the m7G-cap structure. The transcription start site nucleotide is ribose methylated (Nm) in many eukaryotes, whereas an adenosine at this position is further methylated at the N6 position (m6A) by the mammalian Phosphorylated C-terminal domain (CTD)-interacting Factor 1 (PCIF1) to generate m6Am. Here, we show that although the loss of cap-specific m6Am in mice does not affect viability or fertility, the Pcif1 mutants display reduced body weight. Transcriptome analyses of mutant mouse tissues support a role for the cap-specific m6Am modification in stabilizing transcripts. In contrast, the Drosophila Pcif1 is catalytically dead, but like its mammalian counterpart, it retains the ability to associate with the Ser5-phosphorylated CTD of RNA polymerase II (RNA Pol II). Finally, we show that the Trypanosoma Pcif1 is an m6Am methylase that contributes to the N6,N6,2′-O-trimethyladenosine (m62Am) in the hypermethylated cap4 structure of trypanosomatids. Thus, PCIF1 has evolved to function in catalytic and non-catalytic roles.en_US
dc.language.isoengen_US
dc.publisherCell Pressen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.titleThe Mammalian Cap-Specific m<sup>6</sup>Am RNA Methyltransferase PCIF1 Regulates Transcript Levels in Mouse Tissuesen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.pagenumber0en_US
dc.source.volume32en_US
dc.source.journalCell reportsen_US
dc.source.issue7en_US
dc.identifier.doi10.1016/j.celrep.2020.108038
dc.identifier.cristin1877919
dc.description.localcodeThis article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed.en_US
dc.source.articlenumber108038en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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