dc.contributor.author | Subbannayya, Yashwanth | |
dc.contributor.author | Haug, Markus | |
dc.contributor.author | Pinto, Sneha | |
dc.contributor.author | Mohanty, Varshasnata | |
dc.contributor.author | Meås, Hany Zakaria | |
dc.contributor.author | Flo, Trude Helen | |
dc.contributor.author | Prasad, T. S. Keshava | |
dc.contributor.author | Kandasamy, Richard Kumaran | |
dc.date.accessioned | 2021-02-12T09:39:59Z | |
dc.date.available | 2021-02-12T09:39:59Z | |
dc.date.created | 2021-01-05T11:55:46Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | https://hdl.handle.net/11250/2727633 | |
dc.description.abstract | CD4+ T cells (T helper cells) are cytokine-producing adaptive immune cells that activate or regulate the responses of various immune cells. The activation and functional status of CD4+ T cells is important for adequate responses to pathogen infections but has also been associated with auto-immune disorders and survival in several cancers. In the current study, we carried out a label-free high-resolution FTMS-based proteomic profiling of resting and T cell receptor-activated (72 h) primary human CD4+ T cells from peripheral blood of healthy donors as well as SUP-T1 cells. We identified 5237 proteins, of which significant alterations in the levels of 1119 proteins were observed between resting and activated CD4+ T cells. In addition to identifying several known T-cell activation-related processes altered expression of several stimulatory/inhibitory immune checkpoint markers between resting and activated CD4+ T cells were observed. Network analysis further revealed several known and novel regulatory hubs of CD4+ T cell activation, including IFNG, IRF1, FOXP3, AURKA, and RIOK2. Comparison of primary CD4+ T cell proteomic profiles with human lymphoblastic cell lines revealed a substantial overlap, while comparison with mouse CD+ T cell data suggested interspecies proteomic differences. The current dataset will serve as a valuable resource to the scientific community to compare and analyze the CD4+ proteome. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | MDPI | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | The Proteomic Landscape of Resting and Activated CD4+ T Cells Reveal Insights into Cell Differentiation and Function | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.subject.nsi | VDP::Medisinske fag: 700 | en_US |
dc.subject.nsi | VDP::Midical sciences: 700 | en_US |
dc.source.volume | 22 | en_US |
dc.source.journal | International Journal of Molecular Sciences | en_US |
dc.source.issue | 1 | en_US |
dc.identifier.doi | https://doi.org/10.3390/ijms22010275 | |
dc.identifier.cristin | 1865446 | |
dc.relation.project | Samarbeidsorganet mellom Helse Midt-Norge og NTNU: 90176000 | en_US |
dc.relation.project | Norges forskningsråd: 223255 | en_US |
dc.description.localcode | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |