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dc.contributor.authorHakvåg, Sigrid
dc.contributor.authorNærdal, Ingemar
dc.contributor.authorHeggeset, Tonje Marita Bjerkan
dc.contributor.authorKristiansen, Kåre Andre
dc.contributor.authorAasen, Inga Marie
dc.contributor.authorBrautaset, Trygve
dc.date.accessioned2020-05-15T08:08:46Z
dc.date.available2020-05-15T08:08:46Z
dc.date.created2020-05-14T09:47:15Z
dc.date.issued2020
dc.identifier.citationFrontiers in Microbiology. 2020, .en_US
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/11250/2654570
dc.description.abstractThe facultative methylotroph Bacillus methanolicus MGA3 has previously been genetically engineered to overproduce the amino acids L-lysine and L-glutamate and their derivatives cadaverine and γ-aminobutyric acid (GABA) from methanol at 50°C. We here explored the potential of utilizing the sugar alcohol mannitol and seaweed extract (SWE) containing mannitol, as alternative feedstocks for production of chemicals by fermentation using B. methanolicus. Extracts of the brown algae Saccharina latissima harvested in the Trondheim Fjord in Norway were prepared and found to contain 12–13 g/l of mannitol, with conductivities corresponding to a salt content of ∼2% NaCl. Initially, 12 B. methanolicus wild type strains were tested for tolerance to various SWE concentrations, and some strains including MGA3 could grow on 50% SWE medium. Non-methylotrophic and methylotrophic growth of B. methanolicus rely on differences in regulation of metabolic pathways, and we compared production titers of GABA and cadaverine under such growth conditions. Shake flask experiments showed that recombinant MGA3 strains could produce similar and higher titers of cadaverine during growth on 50% SWE and mannitol, compared to on methanol. GABA production levels under these conditions were however low compared to growth on methanol. We present the first fed-batch mannitol fermentation of B. methanolicus and production of 6.3 g/l cadaverine. Finally, we constructed a recombinant MGA3 strain synthesizing the C30 terpenoids 4,4′-diaponeurosporene and 4,4′-diapolycopene, experimentally confirming that B. methanolicus has a functional methylerythritol phosphate (MEP) pathway. Together, our results contribute to extending the range of both the feedstocks for growth and products that can be synthesized by B. methanolicus.en_US
dc.language.isoengen_US
dc.publisherFrontiersen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleProduction of Value-Added Chemicals by Bacillus methanolicus Strains Cultivated on Mannitol and Extracts of Seaweed Saccharina latissima at 50Cen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.pagenumber13en_US
dc.source.journalFrontiers in Microbiologyen_US
dc.identifier.doi10.3389/fmicb.2020.00680
dc.identifier.cristin1810925
dc.description.localcodeCopyright © 2020 Hakvåg, Nærdal, Heggeset, Kristiansen, Aasen and Brautaset. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2


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