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dc.contributor.authorBeal, J, et al.
dc.contributor.authorLale, Rahmi
dc.date.accessioned2020-04-29T07:39:01Z
dc.date.available2020-04-29T07:39:01Z
dc.date.created2016-10-05T08:03:57Z
dc.date.issued2016
dc.identifier.citationPLOS ONE. 2016, 11 (3)en_US
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/11250/2652854
dc.description.abstractWe present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices.en_US
dc.language.isoengen_US
dc.publisherPLOS, Public Library of Scienceen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleReproducibility of fluorescent expression from engineered biological constructs in E. coli.en_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.volume11en_US
dc.source.journalPLOS ONEen_US
dc.source.issue3en_US
dc.identifier.doi10.1371/journal.pone.0150182
dc.identifier.cristin1389502
dc.description.localcodeCopyright: © 2016 Beal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal