dc.contributor.author | Hamilton, Brett R. | |
dc.contributor.author | Marshall, David L. | |
dc.contributor.author | Casewell, Nicholas R. | |
dc.contributor.author | Harrison, Robert A. | |
dc.contributor.author | Blanksby, Stephen J. | |
dc.contributor.author | Undheim, Eivind Andreas Baste | |
dc.date.accessioned | 2020-03-27T09:46:09Z | |
dc.date.available | 2020-03-27T09:46:09Z | |
dc.date.created | 2020-01-14T08:44:03Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Angewandte Chemie International Edition. 2019, 59 . | en_US |
dc.identifier.issn | 1433-7851 | |
dc.identifier.uri | https://hdl.handle.net/11250/2649048 | |
dc.description.abstract | Enzymes are central components of most physiological processes, and are consequently implicated in various pathologies. High‐resolution maps of enzyme activity within tissues therefore represent powerful tools for elucidating enzymatic functions in health and disease. Here, we present a novel mass spectrometry imaging (MSI) method for assaying the spatial distribution of enzymatic activity directly from tissue. MSI analysis of tissue sections exposed to phospholipid substrates produced high‐resolution maps of phospholipase activity and specificity, which could subsequently be compared to histological images of the same section. Functional MSI thus represents a new and generalisable method for imaging biological activity in situ. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Wiley | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Mapping enzyme activity on tissue by functional mass spectrometry imaging | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 4 | en_US |
dc.source.volume | 59 | en_US |
dc.source.journal | Angewandte Chemie International Edition | en_US |
dc.identifier.doi | 10.1002/anie.201911390 | |
dc.identifier.cristin | 1771961 | |
dc.description.localcode | © 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |