Vis enkel innførsel

dc.contributor.authorStødle, Guro
dc.contributor.authorSilva, Gabriela
dc.contributor.authorTangerås, Line Haugstad
dc.contributor.authorGierman, Lobke
dc.contributor.authorNervik, Ingunn
dc.contributor.authorDahlberg, Unn
dc.contributor.authorSun, Chen
dc.contributor.authorAune, Marie Hjelmseth
dc.contributor.authorThomsen, Liv Cecilie Vestrheim
dc.contributor.authorBjørge, Line
dc.contributor.authorIversen, Ann-Charlotte
dc.date.accessioned2020-03-17T10:55:16Z
dc.date.available2020-03-17T10:55:16Z
dc.date.created2018-08-21T15:50:36Z
dc.date.issued2018
dc.identifier.citationClinical and Experimental Immunology. 2018, 193 (1), 84-94.nb_NO
dc.identifier.issn0009-9104
dc.identifier.urihttp://hdl.handle.net/11250/2647154
dc.description.abstractPre‐eclampsia is associated with increased levels of cholesterol and uric acid and an inflamed placenta expressing danger‐sensing pattern recognition receptors (PRRs). Crystalline cholesterol and uric acid activate the PRR Nod‐like receptor protein (NLRP)3 inflammasome to release interleukin (IL)‐1β and result in vigorous inflammation. We aimed to characterize crystal‐induced NLRP3 activation in placental inflammation and examine its role in pre‐eclampsia. We confirmed that serum total cholesterol and uric acid were elevated in pre‐eclamptic compared to healthy pregnancies and correlated positively to high sensitivity C‐reactive protein (hsCRP) and the pre‐eclampsia marker soluble fms‐like tyrosine kinase‐1 (sFlt‐1). The NLRP3 inflammasome pathway components (NLRP3, caspase‐1, IL‐1β) and priming factors [complement component 5a (C5a) and terminal complement complex (TCC)] were co‐expressed by the syncytiotrophoblast layer which covers the placental surface and interacts with maternal blood. The expression of IL‐1β and TCC was increased significantly and C5a‐positive regions in the syncytiotrophoblast layer appeared more frequent in pre‐eclamptic compared to normal pregnancies. In‐vitro activation of placental explants and trophoblasts confirmed NLRP3 inflammasome pathway functionality by complement‐primed crystal‐induced release of IL‐1β. This study confirms crystal‐induced NLRP3 inflammasome activation located at the syncytiotrophoblast layer as a mechanism of placental inflammation and suggests contribution of enhanced NLRP3 activation to the harmful placental inflammation in pre‐eclampsia.nb_NO
dc.language.isoengnb_NO
dc.publisherWiley for The British Society for Immunologynb_NO
dc.titlePlacental inflammation in pre-eclampsia by Nod-like receptor protein (NLRP)3 inflammasome activation in trophoblastsnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber84-94nb_NO
dc.source.volume193nb_NO
dc.source.journalClinical and Experimental Immunologynb_NO
dc.source.issue1nb_NO
dc.identifier.doi10.1111/cei.13130
dc.identifier.cristin1603608
dc.relation.projectNorges forskningsråd: 223255nb_NO
dc.description.localcodeFree Accessnb_NO
cristin.unitcode1920,13,0,0
cristin.unitcode194,65,15,0
cristin.unitnameKvinneklinikken
cristin.unitnameInstitutt for klinisk og molekylær medisin
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel