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dc.contributor.advisorAfset, Jan Egilnb_NO
dc.contributor.authorRasmussen, Tilde Harridsleffnb_NO
dc.date.accessioned2014-12-19T14:20:03Z
dc.date.available2014-12-19T14:20:03Z
dc.date.created2014-04-24nb_NO
dc.date.issued2013nb_NO
dc.identifier713902nb_NO
dc.identifier.urihttp://hdl.handle.net/11250/263939
dc.description.abstractSTEC (Shiga toxin-producing E.coli) are important human pathogens, which can potentially cause hemolytic uremic syndrome (HUS). An important predictor of serious disease is the production of the variant Shiga toxin 2 (Stx2). This thesis is part of a larger project that aims to establish a method forquantitation of stx2 in the form of a real-time PCR assay. We conducted experiments to find optimal culture conditions, performed DNA extraction and validation of PCR results. Furthermore, we have optimized the RT-PCR for both stx2 and the housekeeping gene tufA, and designed standard curves for calculating the efficiency of both of these. In this project we have produced experimental results which in the future can be used in the further development of an RT-PCR assay for stx2.nb_NO
dc.languageengnb_NO
dc.publisherNorges teknisk-naturvitenskapelige universitet, Det medisinske fakultet, Institutt for laboratoriemedisin, barne- og kvinnesykdommernb_NO
dc.titleEstablishing a real-time PCR method for analysis of stx2 expressionnb_NO
dc.typeMaster thesisnb_NO
dc.source.pagenumber32nb_NO
dc.contributor.departmentNorges teknisk-naturvitenskapelige universitet, Det medisinske fakultet, Institutt for laboratoriemedisin, barne- og kvinnesykdommernb_NO


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