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dc.contributor.authorMintz, Paul J
dc.contributor.authorSætrom, Pål
dc.contributor.authorReebye, Vikash
dc.contributor.authorLundbæk, Marie Benner
dc.contributor.authorLao, Kaiqin
dc.contributor.authorRossi, John J
dc.contributor.authorGaensler, Karin ML
dc.contributor.authorKasahara, Noriyuki
dc.contributor.authorNicholls, Joanna P
dc.contributor.authorJensen, Steen
dc.contributor.authorHaoudi, Abdelali
dc.contributor.authorEmara, Mohamed M
dc.contributor.authorGordon, Myrtle YA
dc.contributor.authorHabib, Nagy A
dc.date.accessioned2019-10-16T15:51:51Z
dc.date.available2019-10-16T15:51:51Z
dc.date.created2012-10-24T09:15:28Z
dc.date.issued2012
dc.identifier.citationMolecular Therapy - Nucleic Acids. 2012, 1 (e34), .nb_NO
dc.identifier.issn2162-2531
dc.identifier.urihttp://hdl.handle.net/11250/2622658
dc.description.abstractExploiting the properties of stem cells by microRNA (miRNA) profiling offers an attractive approach to identify new regulators of stem cell fate. Although numerous miRNA have been screened from hematopoietic stem cells (HSC), the targets corresponding to many of these miRNA have not yet been fully elucidated. By miRNA profiling in a subpopulation of CD34+ cells isolated from peripheral blood, we have identified eight clusters of miRNA that were differentially expressed. Further analysis of one of the clusters by bioinformatics revealed that a miRNA, miR-181a*, which is highly expressed in the adherent CD34+ cells, affects the expression levels of Nanog, a stem cell surrogate marker. We show specifically by reporter assay and mutational analysis that miR-181a* targets a seedless 3′ compensatory site in the 3′UTR of Nanog and affects gene expression. We demonstrate that inhibiting miR-181a* upregulates the Nanog expression level, in addition to an increase in alkaline phosphatase activity. Our studies suggest that miR-181a* may be important in controlling the expression level of Nanog in a subpopulation of CD34+ cells.nb_NO
dc.language.isoengnb_NO
dc.publisherElseviernb_NO
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.no*
dc.titleMicroRNA-181a* Targets Nanog in a Subpopulation of CD34+ Cells Isolated From Peripheral Bloodnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber7nb_NO
dc.source.volume1nb_NO
dc.source.journalMolecular Therapy - Nucleic Acidsnb_NO
dc.source.issuee34nb_NO
dc.identifier.doi10.1038/mtna.2012.29
dc.identifier.cristin952349
dc.description.localcodeCopyright © 2012 American Society of Gene & Cell Therapy. Published by Elsevier Inc. Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0)nb_NO
cristin.unitcode194,65,15,0
cristin.unitcode194,63,10,0
cristin.unitnameInstitutt for klinisk og molekylær medisin
cristin.unitnameInstitutt for datateknologi og informatikk
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal