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dc.contributor.authorArum, Carl-Jørgen
dc.contributor.authorGederaas, Odrun Arna
dc.contributor.authorLarsen, Eivind La Puebla
dc.contributor.authorRandeberg, Lise Lyngsnes
dc.contributor.authorHjelde, Astrid
dc.contributor.authorKrokan, Hans Einar
dc.contributor.authorSvaasand, Lars O.
dc.contributor.authorChen, Duan
dc.contributor.authorZhao, Chun-Mei
dc.date.accessioned2019-10-10T11:30:36Z
dc.date.available2019-10-10T11:30:36Z
dc.date.created2011-08-31T10:30:40Z
dc.date.issued2011
dc.identifier.citationJournal of Biomedical Optics. 2011, 16 (2), .nb_NO
dc.identifier.issn1083-3668
dc.identifier.urihttp://hdl.handle.net/11250/2621390
dc.description.abstractOrthotopic bladder cancer model in rats mimics human bladder cancer with respect to urothelial tumorigenesis and progression. Utilizing this model at pT1 (superficial stage), we analyze the tissue responses to hexyl 5-aminolevulinate-induced photodynamic therapy (HAL-PDT). In comparison to untreated rats, HAL-PDT causes little change in tumor-free rat bladder but induces inflammatory changes with increased lymphocytes and mononuclear cell infiltration in rat bladders with tumor. Immunohistochemistry reveals that HAL-PDT is without effect on proliferating cell nuclear antigen expression within the tumor and increases caspase-3 expression in both normal urothelium and the tumor. Transmission electron microscopy reveals severe mitochondrial damage, formations of apoptotic bodies, vacuoles, and lipofuscin bodies, but no microvillus-formed niches in HAL-PDT-treated bladder cancer rats. Bioinformatics analysis of the gene expression profile indicates an activation of T-cell receptor signaling pathway in bladder cancer rats without PDT. HAL-PDT increases the expression of CD3 and CD45RA in the tumor (determined by immunohistochemistry). We suggest that pathways of action of HAL-PDT may include, at least, activations of mitochondrial apoptosis and autophagy, breakdown of cancer stem cell niches, and importantly, enhancement of T-cell activation.nb_NO
dc.language.isoengnb_NO
dc.publisherSociety of Photo-optical Instrumentation Engineers (SPIE)nb_NO
dc.titleTissue responses to hexyl 5-aminolevulinate-induced photodynamic treatment in syngeneic orthotopic rat bladder cancer model: possible pathways of actionnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber6nb_NO
dc.source.volume16nb_NO
dc.source.journalJournal of Biomedical Opticsnb_NO
dc.source.issue2nb_NO
dc.identifier.doi10.1117/1.3536536
dc.identifier.cristin835813
dc.description.localcodeOpen Accessnb_NO
cristin.unitcode194,65,15,0
cristin.unitcode1920,2,0,0
cristin.unitcode194,63,35,0
cristin.unitcode194,65,25,0
cristin.unitnameInstitutt for klinisk og molekylær medisin
cristin.unitnameKirurgisk klinikk
cristin.unitnameInstitutt for elektroniske systemer
cristin.unitnameInstitutt for sirkulasjon og bildediagnostikk
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2


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