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dc.contributor.advisorMenno P. Witter
dc.contributor.advisorMaximiliano José Nigro
dc.contributor.authorKasper Kjelsberg
dc.date.accessioned2019-10-09T14:00:10Z
dc.date.issued2019
dc.identifierno.ntnu:inspera:44804406:3491993
dc.identifier.urihttp://hdl.handle.net/11250/2621235
dc.descriptionFull text available on 2022-06-01
dc.description.abstract
dc.description.abstractAbstract The perirhinal cortex (PER) is an association area belonging to the medial temporal lobe and is situated along the rhinal sulcus on the lateral surface of rodent brain. Perirhinal cortex can be divided into area 35 and area 36, with area 35 comprising the ventral portion of PER and area 36 the dorsal part. Object recognition memory is the most associated function of PER. Lesions in the PER results in detrimental performance when making choices based on previously learned objects, as if the familiar objects have become novel. Supporting the PERs role in object recognition is the extensive connections with other brain areas. All sensory modalities project unisensory information directly to the PER, but also multisensory information reaches the PER through other association areas. Information from PER is then passed on to the lateral entorhinal cortex (LEC), as well as the CA1-field and the subiculum of the hippocampal formation directly, channelling information regarding objects into the hippocampus. A great deal of research has been made regarding PER and the role of visual processing on object recognition. Little has been made on the role of the somatosensory system however, which serves as an important modality for object recognition in rodents. The aim of this study is to characterize the projections from the somatosensory system, namely primary somatosensory cortex (S1), the barrel cortex of S1, and supplementary somatosensory cortex (S2) to the PER. This was achieved by injecting the retrograde tracer Cholera toxin subunit b in different parts of the PER along the rostro-caudal axis and count the labelled cells in somatosensory areas. Additional experiments included injecting the anterograde tracer AAV1-CAG-tdTomato into the barrel cortex. Coronal sections were stained with NeuN and analysed using fluorescence microscopy. Results showed labelled cells in all somatosensory areas, with S2 containing the largest proportion, followed by barrel cortex and S1. Labelled cells were found mostly in layer II/III and layer Va, however, the relative proportion of labelled cells between these two layers depended on depth of the injection. No significant difference in amount of labelled cells in somatosensory areas were found depending on rostro-caudal level of injection site. Anterograde experiments showed labelled fibres terminating in area 35 in the rostral half of PER. These results are comparable to similar findings in rats.
dc.languageeng
dc.publisherNTNU
dc.titleProjections from somatosensory areas to perirhinal cortex in the mouse
dc.typeMaster thesis


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